Millet waxy gene cosegregation molecular marker and detection method thereof

A molecular marker and waxy technology, applied in the field of molecular genetics, can solve problems such as poor agronomic traits, achieve accurate identification and distinction, and facilitate identification.

Active Publication Date: 2012-10-24
GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Shilixiang is a high-quality glutinous mille

Method used

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  • Millet waxy gene cosegregation molecular marker and detection method thereof
  • Millet waxy gene cosegregation molecular marker and detection method thereof
  • Millet waxy gene cosegregation molecular marker and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0051] Example 1. Obtaining a molecular marker for identifying waxy millet containing type IV waxy gene

[0052] 1. Test materials

[0053] Using millet high-quality waxy resources Shilixiang and stem quality variety Yugu No. 1 and its hybrid F 1 , F 2 group.

[0054] 2. Identification of Waxy Characters

[0055] Randomly take 15 mature grains from each material, place them in a small mortar and mash them with a pestle, add iodine-potassium iodide solution (2 g KI in 1 mL of water, add 1 g I after dissolution) 2 , Shake to completely dissolve, add distilled water to 300 mL, store in a brown glass bottle, dilute it 10 times when used) 30 mL, mix the sample and solution with a pestle, and observe the change of starch color in the endosperm.

[0056] 3. Waxy genetic analysis population

[0057] In 2006, the glutinous millet resource Shilixiang was used to prepare a cross-reverse hybrid F with the stem quality Yugu No. 1 1 , Planted F in 2007 1 And harvest F 2 Seed, planted Orthogonal F in 2...

Example Embodiment

[0099] Example 2: Marker genotype analysis of millet waxy materials using markers

[0100] From millet germplasm resources, we collected 100 millet resources recorded as waxy. The origins include Hebei, Henan, Beijing, Tianjin, Southeastern Shanxi, Hubei, Gansu, Shanxi and other regions, which are preserved by our research team.

[0101] Take 5 seeds from each material for dyeing with iodine solution. If both blue and brownish red appear, then 5 seeds are randomly selected for dyeing observation. Among them, the seed endosperm of 59 materials all turned dark blue when exposed to iodine, indicating that these materials were all stems; the seed endosperm of 28 materials all turned brown-red when exposed to iodine, indicating that these 28 materials were waxy materials; For millet material, the endosperm of the seed turns blue or brownish red in contact with iodine, indicating that the material is impure.

[0102] We used two pairs of InDel tags to analyze 100 millet materials with wax...

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Abstract

The present invention relates to a millet waxy gene cosegregation molecular marker and a detection method thereof, which are used for utilization of millet waxy resource and breeding of a novel waxy variety. The molecular marker is an InDel molecular marker, and an amplification primer is a waxy-TSI2 / int1 primer combination and a waxy-int1-1F / 4R primer combination.

Description

technical field [0001] The invention provides a molecular marker identification method of type IV waxy gene of millet, belongs to the field of molecular genetics, and is specially used for the molecular identification of type IV waxy genotype of millet. Background technique [0002] Millet is called millet after shelling. The main substance of millet is starch, with a content of about 75%. The amylose content of general varieties is more than 12.8%, and the amylose content of waxy varieties is between 1.24% and 7%. Glutinous millet grains are small, light yellow or dark yellow in color, and hard in texture. The molecular weight of glutinous millet starch is more than 20 times smaller than that of ordinary millet, and its edible digestibility is more than 20% higher than that of ordinary millet. It also has high viscosity and good The palatability of the finished product has a sweet flavor. Waxy millet starch treated with heating has high swelling power and transparency, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 白辉李志勇朱彦彬全建章曹伟月董立马继芳刘磊郑直董志平
Owner GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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