Molecular marker for increasing barley harvest index QTL site under low phosphor condition and application thereof
A molecular marker, harvest index technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of high utilization value, high success rate and high accuracy
Active Publication Date: 2018-03-02
SICHUAN AGRI UNIV
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Problems solved by technology
However, the current research is limited to the mining potential of crop yield under general conditions, and there are few reports on the gene mining related to crop yield potential under different conditions, especially low phosphorus conditions.
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Embodiment 2
[0059] Example 2 Application of Molecular Marker Hvc316 Tightly Linked to Barley Harvest Index QTL Site Qhi.sau-3H
[0060] 1. Extraction of DNA
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Abstract
The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.
Description
technical field [0001] The present invention relates to the fields of plant nutrition and barley molecular breeding, in particular, relates to a SSR molecular marker and its application for improving barley harvest index QTL locus Qhi.sau-3H closely linked under the condition of low phosphorus in soil. Background technique [0002] Barley (Hordeum vulgare L.) is one of the important cereal crops. It is planted all over the world and ranks fifth in the world's crop production. ten thousand mu. [0003] Phosphorus is an important guarantee for high-yield and high-quality crops, and its status in agricultural production is increasingly prominent. The low content of available phosphorus in the soil is one of the important factors that limit the increase of crop yield. According to reports, 30% to 40% of the cultivated land in the world is severely inhibited in crop yield due to soil phosphorus deficiency. In terms of agricultural production, in order to solve the problem of in...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈光登侯瑶刘亚西李廷轩王婧月胡德益高尚卿余海英马建
Owner SICHUAN AGRI UNIV
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