Molecular marker closely linked to wheat grain filling rate QTL QGfr.sicau-6D and application of molecular marker
A wheat grain and molecular marker technology, which is applied in the fields of molecular biology and genetic breeding to achieve the effects of convenient and stable amplification, accurate and efficient detection, and high accuracy
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0053] Example 1 Acquisition of wheat grain filling rate QTL QGfr.sicau-6D and molecular marker K603
[0054] In the present invention, the wheat grain filling rate QTL QGfr.sicau-6D and molecular marker K603 are obtained by the following methods:
[0055] (1) Using the rapid grain-filling wheat H461 as the female parent and crossing with the wheat Chuannong 16 as the male parent, the hybrid F1 was obtained. The F1 generation single plant selfed to obtain F2, and the single seed transmission method was used to obtain the F8 generation containing 249 lines. In the RIL population, 188 lines were randomly selected to form a genetic mapping population.
[0056] (2) Extract the DNA of each strain of the genetic mapping population by the CTAB method, use Illumina 90K SNP chip technology, and use the DNA of parent H461 and Chuannong 16 as templates to perform genotyping to obtain the RIL population Genotype data. The band pattern of parent H461 is denoted as A, and the band pattern of par...
Embodiment 2
[0071] Example 2 Application of molecular marker K603 closely linked to wheat grain filling QTL QGfr.sicau-6D
[0072] 1. DNA extraction
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com