Molecular marker closely linked to wheat grain filling rate QTL QGfr.sicau-6D and application of molecular marker

A wheat grain and molecular marker technology, which is applied in the fields of molecular biology and genetic breeding to achieve the effects of convenient and stable amplification, accurate and efficient detection, and high accuracy

Active Publication Date: 2020-09-11
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the grain filling rate of wheat variety Chuannong 16 was significantly lower than that of H461

Method used

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  • Molecular marker closely linked to wheat grain filling rate QTL QGfr.sicau-6D and application of molecular marker
  • Molecular marker closely linked to wheat grain filling rate QTL QGfr.sicau-6D and application of molecular marker
  • Molecular marker closely linked to wheat grain filling rate QTL QGfr.sicau-6D and application of molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Example 1 Acquisition of wheat grain filling rate QTL QGfr.sicau-6D and molecular marker K603

[0054] In the present invention, the wheat grain filling rate QTL QGfr.sicau-6D and molecular marker K603 are obtained by the following methods:

[0055] (1) Using the rapid grain-filling wheat H461 as the female parent and crossing with the wheat Chuannong 16 as the male parent, the hybrid F1 was obtained. The F1 generation single plant selfed to obtain F2, and the single seed transmission method was used to obtain the F8 generation containing 249 lines. In the RIL population, 188 lines were randomly selected to form a genetic mapping population.

[0056] (2) Extract the DNA of each strain of the genetic mapping population by the CTAB method, use Illumina 90K SNP chip technology, and use the DNA of parent H461 and Chuannong 16 as templates to perform genotyping to obtain the RIL population Genotype data. The band pattern of parent H461 is denoted as A, and the band pattern of par...

Embodiment 2

[0071] Example 2 Application of molecular marker K603 closely linked to wheat grain filling QTL QGfr.sicau-6D

[0072] 1. DNA extraction

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Abstract

The invention provides a molecular marker closely linked to the wheat grain filling rate QTL QGfr.sicau-6D. The molecular marker is K603, and the nucleotide sequence of the molecular marker is as shown by SEQ ID NO.1. The molecular marker K603 is closely linked to the wheat grain filling rate QTL. Detection analysis shows that the molecular marker can be used for accurately tracking the wheat grain filling rate QTL and predicting the wheat grain filling rate characteristics to further provide convenience for molecular design breeding. Through detection, the molecular marker K603 can enhance the accuracy of the wheat grain filling rate prediction, so that the wheat varieties or strains with the grain filling rate increasing QTL can be fast screened for breeding, and the breeding progress ofthe wheat high-yield varieties can be greatly accelerated.

Description

Technical field [0001] The present invention relates to the field of molecular biology and genetic breeding, in particular, to a molecular marker K603 closely linked with wheat grain filling rate QTL QGfr.sicau-6D and its application. Background technique [0002] Wheat (Triticum aestivum L.) is one of the important food crops in the world, and more than 40% of the population uses wheat as a staple food material. In our country, wheat occupies an extremely important position in both agricultural production and food processing and is one of the most important food crops. The filling rate and duration of wheat grains directly affect the final size, quality and weight of the grains, and ultimately determine the yield of wheat. Filling characteristics not only affect the grain weight, but also are closely related to the final grain size, fullness index and commercial properties. Filling rate refers to the accumulation of grain dry matter per unit time, which shows a "slow-fast-slow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2563/107C12Q2531/113
Inventor 刘亚西林宇蒋孝军李彩霞陶阳杨希兰王智强武方琨
Owner SICHUAN AGRI UNIV
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