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Molecular marker closely linked to wheat grain filling rate qtl QGfr.sicau-7D.1 and its application

A wheat grain and molecular marker technology, applied in the fields of molecular biology and genetic breeding, achieves the effects of high accuracy, high utilization value, and improved selection and identification efficiency

Active Publication Date: 2022-07-08
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the grain filling rate of wheat variety Chuannong 16 was significantly lower than that of H461

Method used

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  • Molecular marker closely linked to wheat grain filling rate qtl QGfr.sicau-7D.1 and its application
  • Molecular marker closely linked to wheat grain filling rate qtl QGfr.sicau-7D.1 and its application
  • Molecular marker closely linked to wheat grain filling rate qtl QGfr.sicau-7D.1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Acquisition of QTL QGfr.sicau-7D.1 for grain filling rate of wheat and molecular marker KASP705

[0054] In the present invention, the wheat grain filling rate QTL QGfr.sicau-7D.1 and molecular marker KASP705 are obtained by the following methods:

[0055] (1) Using the fast grain filling wheat H461 as the female parent, and the wheat Chuannong 16 as the male parent, the hybrid F1 was obtained. The F1 generation was selfed to obtain F2, and the single-seed propagation method was used to obtain the F8 generation containing 249 lines. In the RIL population, 188 lines were randomly selected to constitute the genetic mapping population.

[0056] (2) Extract the DNA of each line of the genetic mapping population by CTAB method, use Illumina 90K SNP chip technology, and use the DNA of parents H461 and Chuannong 16 as templates to perform genotyping to obtain the RIL population genotype data. The band type of the parent H461 was denoted as A, and the band type of t...

Embodiment 2

[0071] Example 2 Application of molecular marker KASP705 closely linked to wheat grain filling QTL QGfr.sicau-7D.1

[0072] 1. DNA extraction

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PUM

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Abstract

The invention provides a molecular marker closely linked to the wheat grain filling rate QTL QGfr.sicau-7D.1, the molecular marker is KASP705, and its nucleotide sequence is shown in SEQ ID NO. 1; the molecular marker KASP705 is associated with the wheat grain The filling rate QTLs are tightly linked. Detection and analysis showed that the molecular marker could accurately track the QTL of the grain filling rate of wheat, predict the grain filling rate characteristics of wheat, and then facilitate molecular design breeding. The detection molecular marker KASP705 can enhance the accuracy of wheat grain filling rate prediction, so as to quickly screen wheat varieties or lines with QTL increasing grain filling rate for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties.

Description

technical field [0001] The invention relates to the field of molecular biology and genetic breeding, in particular to a molecular marker KASP705 closely linked with the wheat grain filling rate QTL QGfr.sicau-7D.1 and its application. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in the world, with more than 40% of the population taking wheat as the staple food. In my country, wheat occupies an important position in both agricultural production and food processing, and is one of the most important food crops. The grain filling rate and grain filling duration of wheat grains directly affect the final grain size, quality and grain weight, and ultimately determine the yield of wheat. Grain filling characteristics not only affect grain weight, but are also closely related to final grain size, fullness index, and commerciality. Grain filling rate refers to the grain dry matter accumulation per unit time, which shows a "slow-f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 刘亚西林宇蒋孝军李彩霞陶阳杨希兰王智强武方琨
Owner SICHUAN AGRI UNIV
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