Molecular markers of wheat scab infection resistance gene Fhb4 and application of the molecular markers

A technology of molecular markers and head blight, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of large genetic distance, speed up gene cloning research, etc., and achieve stable amplification and convenient identification , Improve the efficiency of selection and identification

Inactive Publication Date: 2012-07-25
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

In addition, although Fhb4 has been finely mapped, the genetic distance in the interval is still very large. It is necessary to develop more closely linked molecular markers to speed up the cloning research of this gene

Method used

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  • Molecular markers of wheat scab infection resistance gene Fhb4 and application of the molecular markers
  • Molecular markers of wheat scab infection resistance gene Fhb4 and application of the molecular markers
  • Molecular markers of wheat scab infection resistance gene Fhb4 and application of the molecular markers

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Experimental program
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Effect test

Embodiment 1

[0043] The molecular marker of wheat head blight resistance gene Fhb4 was obtained by the following methods:

[0044] (1) Wangshuibai Fhb4 near-isogenic line NMAS007 and its recurrent parent Mianyang 99-323F 2∶3 Creation of populations and screening of Fhb4 segment recombinants:

[0045] (1) NMAS007(♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 4303 individuals 2 group;

[0046] (2) Use the boundary markers Xhbg226 and Xgwm149 of Fhb4 (Torada et al.2006; et al.1998) in F 2 In the population, 187 heterozygous individuals with recombination in this segment were screened, and the same marker was used in its F 3 Generation screening yielded 459 homozygous individuals recombined in this segment.

[0047] (2) Identification of recombinant disease-resistant phenotypes

[0048] Sow susceptible wheat grains in the field about ten days before the recombinants flower to inoculate these homozygous recombinants, and repeat once a week later....

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Abstract

The invention belongs to the field of crop breeding, and discloses molecular markers of a wheat scab infection resistance gene Fhb4 and application of the molecular markers. The molecular markers are selected from any one of MAG5184, MAG7237 and MAG2521, and the genetic distances to Fhb4 gene are 0.72cM, 0.34cM and 0.92cM, respectively. According to the invention, the molecular markers having the closest linkage with Fhb4 are obtained for the first time in the world. These three molecular markers are codominant and have the advantages of convenient detection, and stable, simple and convenient amplification. The molecular marker MAG7237 is used to detect Fhb4 gene to determine the existence and status of Fhb4 and predict the scab resistance of wheat, and then to screen the plants with Fhb4 gene, which are used for breeding disease-resistant varieties.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab infection resistance gene Fhb4 and its application. Background technique [0002] Wheat is one of the most important food crops in the world, providing about 20% of human energy. Many biotic and abiotic stresses directly affect the yield and quality of wheat in wheat production. Scab is one of the most important biological adversities, which seriously affects the quality and yield of wheat. The breeding and application of disease-resistant varieties is the most economical and effective method to control scab. The discovery of disease-resistant genes is the premise and foundation of disease-resistant breeding, and the development of molecular markers closely linked to disease-resistant genes is the most important way to apply disease-resistant genes. key. [0003] There are five main types of wheat scab resistance (Mesterhazy1995), among which resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 马正强薛树林李国强
Owner NANJING AGRICULTURAL UNIVERSITY
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