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SNP Molecular Marker Linked to Wheat Effective Tiller Number QTL and Its Application

A molecular marker and tiller number technology, applied in the fields of molecular biology and crop genetics and breeding, can solve the problem of insufficient molecular markers, achieve high accuracy, improve breeding work efficiency, and improve selection and identification efficiency

Active Publication Date: 2020-12-25
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Previously, some scholars conducted QTL mapping on the effective tiller number and found that the related QTL widely existed in wheat. However, there are not many closely linked molecular markers related to the trait of effective tiller number in wheat that can be used for practical molecular breeding.

Method used

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  • SNP Molecular Marker Linked to Wheat Effective Tiller Number QTL and Its Application
  • SNP Molecular Marker Linked to Wheat Effective Tiller Number QTL and Its Application
  • SNP Molecular Marker Linked to Wheat Effective Tiller Number QTL and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Acquisition of wheat effective tiller number QTL QPTN.sau-4B and its molecular marker KASP-1

[0043] (1) Using the wheat line '20828' as the female parent and the wheat variety 'Chuannong 16' as the male parent, the hybrid F 1 , F 1 F 2 , at F 2 Using the single-ear transmission method, all the way to F 8 Generations, recombinant inbred lines containing 199 lines were obtained to form a genetic mapping population.

[0044] (2) Phenotypic identification of the effective tiller number of recombinant inbred lines: analyze and identify the effective tiller numbers of recombinant inbred lines at the maturity stage of wheat, remove the individual plants at both ends of each row, collect five individual plants with the same growth, and calculate the effective Tiller number, and get the average value, which represents the effective tiller number of the strain.

[0045] (3) 55K SNP chip analysis

[0046] a) DNA extraction: The DNA of the parent '20828', 'Chuanno...

Embodiment 2

[0055] Example 2 Application of Molecular Marker KASP-1 in Selection and Control of Effective Tiller Number QTLQPTN.sau-4B

[0056] (1) Using the common wheat line 'S849-8' with a large number of effective tillers as the female parent and the common wheat line 'Chuannong 16' with a small number of effective tillers as the male parent to construct a recombinant inbred line, and randomly select the offspring lines 54 strains.

[0057] (2) Carry out KASP-1 marker detection on the obtained 54 strains, the specific method is: extract the DNA of 54 strains; use it as a template, and carry out PCR with the specific primer pair of molecular marker KASP-1 as primers To amplify and perform a fluorescent readout, the primers are:

[0058] Primers on the FAM tag: (the underlined part is the FAM tag sequence)

[0059] 5' -GAAGGTGACCAAGTTCATGCTTACAGTCCTTTGAGTTGTTCC -3' (SEQ ID No.1)

[0060] Primers on the HEX tag: (the wavy part is the HEX tag sequence)

[0061]

[0062] Universal...

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Abstract

The invention relates to the field of wheat molecular breeding, and specifically discloses an SNP molecular marker linked to an effective tiller number QTL QPTN. Sau-4B of wheat and application thereof. The molecular marker is KASP-1, and can be obtained by amplification of primers of which nucleotide sequences are shown in SEQ ID NO. 1-3. Detection analysis shows that the molecular marker can beused for accurately tracking the effective tiller number QTL QPTN. Sau-4B of the wheat, predicting the effective tiller number characteristics of the wheat, and further facilitating molecular design breeding. The invention also discloses a molecular marker method for identifying the effective tiller number QTL QPTN. Sau-4B of the wheat, and by adopting the method, the accuracy of prediction of theeffective tiller number can be improved so as to quickly screen out wheat varieties or lines with increased effective tiller number for breeding, and the breeding process of high-yield varieties of the wheat can be greatly speeded up.

Description

technical field [0001] The invention relates to the fields of molecular biology and crop genetic breeding, in particular to a SNP molecular marker linked to wheat effective tiller number QTLQPTN.sau-4B and the application of the molecular marker. Background technique [0002] Common wheat (Triticum aestivum L.) is one of the most important crops in the world, and its sown area accounts for 20%-30% of the country's arable land. It is the staple food of 35% of the population. According to reports, wheat production should increase by 70% to meet future food demand. [0003] At present, the area of ​​arable land in the world is decreasing and the population is increasing. It is urgently expected that the annual wheat output will increase rapidly. Wheat yield components include the number of spikes, the number of grains per spike, and the thousand-grain weight. The number of panicles directly depends on the number of tillers, so the number of tillers has a great impact on the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马建兰秀锦刘家君郑有良魏育明刘亚西江千涛陈国跃陈光登
Owner SICHUAN AGRI UNIV
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