Method for identifying cherry variety

Abstract

The invention discloses a method for identifying a cherry variety, and belongs to the field of molecule identification of cherry varieties. The invention firstly discloses 11 pairs of SSR (Simple Sequence Repeats) primers for identifying the varieties of cherry, wherein the nucleotide sequences of the primers are as shown in SEQ ID No.1 to SEQ ID No.22. By adopting the method, core primer combinations are further screened from the 11 pairs of the SSR primers, 87 cherry germplasms can be distinguished by using 5 core primer combinations, and the obtained primer combinations have the advantages of being high in polymorphism, high in amplification stability, good in repeatability, clear in electrophoretic band and the like. The invention further discloses a cherry variety identification method and a kit based on S genotype information and SSR primer combinations. By adopting the primers, identification upon different cherry varieties can be rapidly and accurately completed. The SSR primer pairs, the cherry variety identification method and the kit disclosed by the invention can be applied to cherry germplasm evaluation, cherry germplasm innovation, parent choice for breeding, and the like.

Description

Technical field [0001] The invention relates to a cherry variety identification method, in particular to a cherry variety identification method based on the combination of S genotype information and SSR core primers, and belongs to the field of molecular identification of cherry varieties. Background technique [0002] In the past 20 years, China's sweet cherry industry has rapidly expanded from traditionally planted Shandong and Liaoning to Henan, Shanxi, Shaanxi, Gansu, Jiangsu and other provinces along the Longhai Railway, as well as cold highlands such as Yunnan, Guizhou and Sichuan. As of the spring of 2016, China’s sweet cherry cultivation area has reached 153,300 ha 2 . However, at present, most of the widely cultivated varieties in various cultivation areas in China are introduced abroad. With the gradual increase in the number of sweet cherry varieties and the increasing frequency of germplasm exchanges, and the established pattern of multiple breeding and multiple manag...

AI Technical Summary

Problems solved by technology

However, at present, most of the varieties widely cultivated in various cultivation areas in China are imported from abroad. With the gradual increase in the number of sweet cherry varieties and the frequent exchange of germplasm, as well as the established pattern of multi-plant breeding and multi-management, different species with the same name or different The name phenomenon is very common, which not only brings many difficulties and confusion to sweet cherry variety breeding and property rights protection, but also causes serious losses to the cherry industry.

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