Molecule marking method for identifying variety purity of insect resistance hybrid cotton Suza No.3

A technology of cultivar purity and labeling method, applied in biochemical equipment and methods, analytical materials, microbial determination/inspection, etc. The effect of speeding up the quality inspection process, efficient and accurate quality control, and high accuracy

Inactive Publication Date: 2008-01-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the process of seed production, if the female parent is not completely detasselled or missed, the selfing bolls formed will seriously affe

Method used

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  • Molecule marking method for identifying variety purity of insect resistance hybrid cotton Suza No.3

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Embodiment Construction

[0029] In the following, the present invention will be further illustrated through the SSR marker analysis examples based on PCR technology.

[0030] The experimental materials in this example are commercial seeds of insect-resistant hybrid cotton "Suza No. 3" and their parent material seeds. Cultivate the seedlings indoors on a sand bed at 25-30°C for 5-7 days, and take the fresh cotyledons.

[0031] Methods: Extract the DNA of cotton cotyledons, use SSR molecular markers to carry out PCR amplification, subject the amplified products to gel electrophoresis, count the results and screen the characteristic primers.

[0032] 1. Extraction of cotton genomic DNA

[0033] (1) Put 1 piece of fresh cotton cotyledons the size of a thumb lid into a 1.5ml centrifuge tube, add 0.6ml extraction buffer [0.35M Glucose, 0.1M Tris-HCl (pH8.0), 5mM Na-EDTA (pH8) .0), 2% PVP, 1% (V / V) β-Me], electro-rotation grinding. Centrifuge at 10,000 rpm for 10 min, discard the supernatant;

[0034] (2) Add 0....

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Abstract

The invention relates to a SSR labeling method to appraisal the authenticity and /or variety purity insect-resistant hybrid cotton suza3, which belongs to biological technology field. The invention takes a commodity of insect-resistant hybrid cotton <SUZA3> and parental DNA as template to screen with SSR molecular marker, which can differentiate two SSR markers of male parent, female parent and hybrid at the same time. A band size of female parent specific marker JZSSSR259205 produced by SSR probe JZSSSR259 is 205bp; female parent specific marker JZSSSR259245 band size is 245bp; a band size of female parent specific marker JZSSSR413125 produced by SSR probe JZSSSR413 is 125bp; male parent specific marker JZSSSR413135 band size is 135bp. The two SSR marker can not only identify the hybrid cotton variety purity and authenticity, but also can do mutual identification which is good for controlling quality accurately and efficiently of the hybrid cotton breed and quicken the quality inspection process to identify quickly to mass sample in short time with a reliable result.

Description

Technical field [0001] The SSR marking method for identifying the authenticity and / or variety purity of the insect-resistant hybrid cotton "Suza 3" of the present invention is effective for cotton F 1 The authenticity identification and / or variety purity identification of the seeds of the hybrid variety "Suza 3" belong to the field of biotechnology. Background technique [0002] Good varieties are the basis for high crop yields, and mixed varieties and reduced purity will significantly reduce yields. Rapid and accurate identification of varieties and purity analysis play an important role in seed quality standardization, variety verification, fake seed identification, and disputes over property rights. At present, my country’s seed production and management are not well regulated. Unqualified seeds are frequently mixed into the market and used for production, resulting in production reductions and economic losses. Therefore, variety purity identification is particularly important...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/447
Inventor 殷剑美陈旭升狄佳春肖松华许乃银刘剑光吴巧娟
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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