Method for separating beta-lactoglobulin from desalted whey powder

A technology for desalting whey powder and lactoglobulin, which is applied in the field of separation and purification of beta-lactoglobulin, can solve the problem of high cost of egg white, and achieve the effects of easy popularization, simplified process and wide application range.

Inactive Publication Date: 2014-07-16
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of egg white is relatively expensive, so it is particularly important to find a low-cost, high-quality substitute for egg white

Method used

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  • Method for separating beta-lactoglobulin from desalted whey powder
  • Method for separating beta-lactoglobulin from desalted whey powder
  • Method for separating beta-lactoglobulin from desalted whey powder

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Embodiment

[0040] 1. Fractional salting out of ammonium sulfate

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Abstract

The invention discloses a method for separating beta-lactoglobulin from desalted whey powder. According to the method, desalted whey powder is used as a treatment object which is subjected to ammonium sulfate salting out, primary ultrafiltration desalting, primary ion-exchange column chromatography, secondary ultrafiltration desalting, secondary ion-exchange column chromatography, gel chromatography and final separation so as to obtain beta-lactoglobulin with relatively high purity. Compared with the prior art, the method has the advantages that firstly, the operation is convenient, the process is simplified, related requirements are lowly required, and the operation can be carried out in a small laboratory; secondly, operators are lowly required, and the popularization and the application are convenient; thirdly, beta-lactoglobulin is relatively high in separation purity, the strip is clear and the separation is thorough; fourthly, the application range is wide, and different varieties of protein can be separated.

Description

technical field [0001] The invention relates to a method for separating and purifying β-lactoglobulin, in particular to a method for separating β-lactoglobulin from desalted whey powder. Background technique [0002] Milk whey was once considered to be the waste produced in the process of milk processing. Due to the high cost of processing whey, it is generally only processed into whey powder or whey protein isolate (whey protein isolate, WPI) and other low-priced commodities. However, with the birth of new technology and processing methods, people have a new understanding of the biological activity of whey protein. Studies have found that whey contains a variety of proteins with nutrition, biological activity and food functionality. Among them, the main component of whey protein, β-lactoglobulin (β-lactoglobulin, β-LG) and its peptides have broad application prospects in food processing of dairy products, meat products and bread production, as well as in the development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/36C07K1/30C07K1/18C07K1/16
CPCC07K14/4717
Inventor 冯郑珂杨章昀潘家荣张永勇
Owner CHINA JILIANG UNIV
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