Microsatellite primers for identifying crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas and hybrid thereof and identification method

Abstract

The invention discloses microsatellite primers for identifying crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas and a hybrid thereof and an identification method.The microsatellite primers are F:5'-CGACTGGTGGGAGTTTCTGAC-3' and R:5'-GCCGCTTCTATCTCCTTTGC-3'.Due to the fact that the external morphology of oysters changes greatly along with different living environments, identification is usually difficult only according to morphological characteristics; the oysters in the larval stage are more difficult to distinguish according to the morphological characteristics.By means of the microsatellite primers and the identification method, crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals can be identified rapidly and accurately, and identification of the hybrid individual of the crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals has the potential application value.Meanwhile, the microsatellite primers for identifying the crassostrea hongkongensis, the crassostrea ariakensis and the crassostrea gigas and the hybrid thereof and the identification method have the advantages of being simple in method, visual, accurate and effective in result, free of influences of environment and growth stage, low in cost and the like.

Description

Technical field: [0001] The invention belongs to the field of shellfish molecular markers and species identification in aquatic biology technology, and specifically relates to microsatellite primers and identification methods for identifying Hong Kong giant oyster, Ariake oyster, Pacific oyster and hybrids thereof. Background technique: [0002] Microsatellite DNA, also known as short tandem repeats (STRs), simple sequence repeats (SSRs), and simple sequence length polymorphism (SSLP), refers to the core sequence composed of 1 to 6 nucleotides in the genome. nucleotide sequence. In recent years, microsatellite markers have been widely used in species identification and hybrid analysis due to their simplicity and efficiency. [0003] Oysters belong to the phylum Molluscs, Bivalves, Pearl Oysters, and Oysteridae, and are globally distributed groups. Oysters are delicious and rich in various essential amino acids. They are an important marine biological resource and the most i...

AI Technical Summary

Problems solved by technology

In recent years, molecular biology based on differences in mitochondrial genes such as COI and 16SrDNA and ITS sequences has been used to identify the three, but the differences between these gene sequences are relatively small, and it is difficult to distinguish them by simple methods such as electrophoresis; although the HRM method is simple, it can Requires the support of related large-scale instruments; while the sequencing test cycle is longer and the cost is higher

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