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ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa

A technique for genetic diversity of Hedyotis diffusa, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc. The effect of scientific value and application value, stable belt shape and low cost

Active Publication Date: 2017-11-17
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

At present, no scholars have used ISSR molecular markers to study the genetic diversity of Hedyotis diffusa

Method used

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  • ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa
  • ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa
  • ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa

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Embodiment Construction

[0029] In order to express the present invention more clearly, the present invention will be further described below through specific examples. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0030] Twenty-three samples of Hedyotis diffusa were collected from seven provinces including Guangxi Zhuang Autonomous Region, Guangdong Province, Hunan Province, Zhejiang Province, Fujian Province, Jiangxi Province and Anhui Province. The sources of the samples are shown in Table 1 below.

[0031] Table 1

[0032]

[0033]

[0034] 1. Extraction of DNA

[0035] The samples of Hedyotis diffusa obtained from 23 different regions shown in Table 1 were extracted by kit method respectively, and the purity, quality and integrity of the total DNA of each variety of sweet potato were detected by 1% ag...

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Abstract

The invention provides an ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa, and belongs to the technical field of molecular biology. The method comprises the following steps of selecting four primers with clear background, good amplification effect and high polymorphism, amplifying genomic DNAs of the hedyotis diffusa by using ISSR-PCR reaction, analyzing stripes according to an ISSR-PCR result, establishing a DNA fingerprint of the hedyotis diffusa, and constructing a genetic resource database of the hedyotis diffusa. According to the method of the invention, a new method for identification of variety of the hedyotis diffusa and analysis of genetic diversity is created, so as to effectively solve the problem of poor representativeness of a database in the construction of the genetic marker database in the past, and also solve the problem that the existing marking technology has a defect in the hedyotis diffusa genetic resources; the method has the advantages of high polymorphism, good repeatability, low cost, and simple testing procedure, and is not affected by an environment.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular biology, in particular to an ISSR molecular marker method for analyzing the genetic diversity of Hedyotis diffusa. 【Background technique】 [0002] Hedyotis diffusa Willd. is a commonly used Chinese herbal medicine in the Zhuang nationality. It is an annual herb with opposite leaves and sessile; the flowers are solitary or twin in the leaf axils, and the pedicel is slightly thick and four in number; the capsule is membranous and oblate; it is widely distributed in In the area south of the Yangtze River in my country, it is more common in humid open lands such as paddy fields. Hedyotis diffusa has the effects of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, diuresis and exuding dampness, reducing swelling and relieving pain. Oral administration is mostly used to treat tumors, snake bites, and malnutrition in children; external application is mainly used to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 田慧封毅周敏高慧新
Owner GUANGXI UNIV OF CHINESE MEDICINE
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