Method for rapidly identifying switchgrass hybrids by SSR (simple sequence repeat) molecular marker technology
A molecular marker, hybrid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as high cost and time-consuming, and achieve high accuracy, easy operation, and good versatility.
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[0022] The present invention will be described in detail below in conjunction with specific embodiments.
[0023] 1.2.1 Test materials: hybrid offspring of switchgrass to be identified and their parent plants or seeds.
[0024] 1.2.2 Genomic DNA extraction
[0025] Using the young leaves or seeds of switchgrass plants to be identified as materials, DNA was extracted using a plant genomic DNA extraction kit (Tiangen Company). The purity of the extracted DNA was tested by 0.8% agarose gel electrophoresis. DNA samples were stored in a -20°C refrigerator. When the experiment was started, a part of each DNA sample was taken out and diluted to 20 ng / μL, and stored in a refrigerator at 4°C for use.
[0026] 1.2.3 SSR Analysis
[0027] Using the screened switchgrass EST-SSR marker primers:
[0028] primer1-F: 5'-AGTTTGAGCTTTGTGTTTTTG-3',
[0029] primer1-R: 5'-CAACAATTTGGTTTCAACAAG-3';
[0030] Perform hybrid identification. The sequence has the characteristics of clear and st...
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