Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

Abstract

Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation.; Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 61,250,809, filed October 12, 2009, and U.S. Provisional Application No. 61 / 278,936, filed October 13, 2009, which are hereby incorporated by reference in their entirety. technical field [0003] The present disclosure relates to methods of providing comparison of tissue sections for pathological determination. Background technique [0004] Microscopic clinical examination of routine histologically stained tissue sections can be used to assess morphological patterns and tissue architecture of diagnostic significance. A skilled physician can observe such histologically stained tissue sections for diagnosis as well as for planning and evaluating treatment. The contrast of structures provided by such images using typical stains is familiar and allows the physician to devote herself to interpreting the features and abnormalities of the anatomical and ...

AI Technical Summary

Problems solved by technology

Comparing consecutive slices not only increases the expense and time necessary for the evaluation, it may be difficult or impossible to correlate features found in one slice with features found in another

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