22 results about "Multi analyte" patented technology

Provided herein are methods, assays and kits for detecting methylated genomic DNA and one or more protein biomarkers in one or more biological samples. In some aspects, the disclosure provides methods, assays and kits for detecting or prognosing a proliferative disease, such as colorectal cancer, in a subject based on the presence of methylated genomic DNA and one or more protein biomarkers in one or more biological samples.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

The invention provides a multi-analyte sensor. The invention also provides a preparation method of the multi-analyte sensor. According to the multi-analyte sensor and the preparation method provided by the invention, at least one working electrode and at least one auxiliary electrode are arranged on each of the two opposite sides of the substrate layer, and the two working electrodes, one reference electrode and one counter electrode required by multi-analyte detection are integrated at one detection end, so that the volume is smaller, and the patient compliance is better. According to the multi-analyte sensor provided by the invention, starting from a double-three-electrode system, the shared auxiliary electrode (reference motor / counter electrode) can meet the system requirement of the first working electrode and can balance the system requirement of the second working electrode, and a steady state is achieved under the condition that current signals are consistent; compared with an existing system which is separated independently and does not share an auxiliary electrode, the system has more advantages.

The invention provides a terahertz microstructure dual-core dual-channel optical fiber ultra-sensitive microfluid sensing device, and relates to the technical field of Zeonex material microstructure optical fibers, and the terahertz microstructure dual-core dual-channel optical fiber ultra-sensitive microfluid sensing device comprises a fiber core subjected to side polishing ring opening treatment, a molybdenum disulfide film, a cladding and five layers of periodically arranged air holes; the cladding comprises an outer thin shell and a middle D-shaped polishing platform; the outer-layer thin shell is connected with the D-shaped polishing platform through a horizontal supporting arm; the horizontal support arm divides the sensing device into two independent sensing channels; the five layers of periodically arranged air holes are uniformly distributed in the D-shaped polishing platform; the molybdenum disulfide film is attached to the open loop and is in direct contact with an external analyte to be detected. According to the design of the sensing device provided by the invention, the multi-analyte detection capability is improved, and a powerful platform is established for real-time biomedical diagnosis and environmental monitoring.

Some embodiments of the disclosure provide a multi-analyte monitoring sensor, which includes a substrate, a working electrode disposed on the substrate, an enzyme sensing layer disposed on the working electrode, and a polymer membrane layer disposed on the enzyme sensing layer. In some examples, the working electrode includes a first working electrode and a second working electrode. The enzyme sensing layer includes a first analyte enzyme layer and a second analyte enzyme layer, the first analyte enzyme layer is disposed on the first working electrode and the second analyte enzyme layer is disposed on the second working electrode, the first analyte is glucose, and the second analyte is ketone. The polymer membrane layer is permeable to both the first analyte and the second analyte and covers the first analyte enzyme layer and the second analyte enzyme layer.

The present disclosure relates in some aspects to methods and compositions for in situ analysis involving preparation and treatment (e.g., de-crosslinking) of biological samples. Preparation of biological samples comprising nucleic acid analytes and protein analytes for detection are provided. Methods, in which the sample with bound probes is contacted with a buffer at a temperature above 80°C, improve the detection of optical signals associated with analytes in a fixed biological sample.

The multifunctional antimonene-gold nano composite material (AM-Au NCs) is rapidly, simply and conveniently prepared and is used for multi-analyte fluorescence sensing (such as metal ions, CD63 and tumor-derived exosomes), molecular information coding and protection. Sodium dodecyl benzene sulfonate, AM, Au < 3 + > and NaBH4 are mixed by using different formulas at room temperature to prepare AM-Au NCs with an adjustable gold nanostructure and characteristics. And interestingness is that AM-Au NCs doped with the gold nanoparticles show wide fluorescence quenching capability on the nanosheets, and the fluorescence quenching capability is remarkably different in different dyes and DNA (deoxyribonucleic acid) consisting of different basic groups. A fluorescent sensing platform constructed by AM-Au NCs is combined with a dye or an aptamer and is used for detecting metal ions, CD63 and tumor-derived exosomes. In real samples, through binary or digital coding of selective response and diversified DNA sequences of sensing systems, a series of molecular encryption and hiding technologies are provided and applied to realize molecular-level protection of special text information.

The present invention relates to detection reagents and electrode arrangements for multi-analyte diagnostic test elements and methods of use thereof. Detection reagents, multi-analyte test elements, test systems, and multi-analyte measurement methods are provided. In particular, a multi-analyte test element has (1) a first working electrode and a first counter electrode pair covered with a first analyte-specific reagent comprising an enzyme, a coenzyme and a first medium, and has (2) a second working electrode covered with a second analyte-specific reagent comprising an enzyme, a coenzyme and a second medium, where the second medium is different from the first medium. A single counter electrode may be used as a counter electrode for both first and second analyte measurements at their respective working electrodes. Further, the medium concentration, the measurement range, and the applied potential difference are different for each analyte-specific measurement.

A physiological state monitoring system includes an implantable multi-analyte sensor probe including a first working electrode configured to generate a first electrical signal indicating concentration of a first analyte and a second working electrode configured to generate a second electrical signal indicating a concentration of a second analyte. Monitor system control circuitry is configured to generate a feature vector that includes one or more of a baseline-subtracted analyte value, a first-order temporal derivative, a second-order temporal derivative, or an inter-analyte ratio based on the digital sensor data, access parameters of a machine-learning classification model trained on historical multi-analyte sensor logs and associated ground-truth physiological states, and use the machine-learning classification model to generate, based on the feature vector, a real-time classification label and an associated confidence value identifying a physiological state.

The present application provides an analyte sensor based on monitoring multiple analytes. The analyte sensor includes a channel structure, and the analyte sensor can be implanted into a human body for simultaneously monitoring multiple analytes, and has the characteristics of good flexibility, small size, coexisting multiple analysis methods, low manufacturing cost, simple process flow, and long-term monitoring. The analyte sensor is configured to simultaneously monitor analytes / indicators, and can achieve the characteristics of two functional areas in one plane at the same time. The introduction of the channel structure of the present application makes the thickness of the analyte sensor more suitable and the wearing experience better. The introduction of the channel structure can also avoid problems such as unstable signal transmission caused by small electrode area or narrow electrode cables, resulting in poor sensor stability.

In a multi-analyte multi-component analysis, multiple kinds of numerical information are obtained for each sample as measurement and data-analysis results for multiple compounds. A threshold-based flag condition is set for each of two or more kinds of numerical information according to user operations. Multiple categories, each having a determination condition including the combination of the presence / absence of the flags corresponding to the two or more kinds of numerical information, are set according to user operations. For each sample-compound combination, each kind of numerical information is tested as to whether it satisfies the flag condition, and is flagged if it satisfies the condition. Each sample-compound combination is tested as to the presence / absence of the flags according to the determination condition of each category, to identify combinations belonging to any category. The identified combinations are displayed, with each combination related to the corresponding category or each category visually distinguished from the other.

A multi-analyte sensor system is disclosed. The system includes a sensor probe that has a first set of electrodes that transduce glucose into electrical signals, a second set of electrodes that transduce lactate into electrical signals and a third set of electrodes that provide working and counter electrode functionality for the first and second set of electrodes. The system has an electronics module that electrically interfaces with the sensor probe, and includes a transceiver configured to transmit sensor data. The system also includes control circuitry communicatively coupled to the electronics module that determines a glucose state based on signals from the first set of electrodes and also determine a lactate state based on signals from the second set of electrodes. The control circuitry also generates an insulin infusion pump control signal based on signals from the first and second set of electrodes.

Disclosed are systems and methods for measuring data from an environment. The system may be an environmental sensor that includes a substrate and four layers. The first layer may include source and drain electrodes. The second layer may include at least three electrodes including a working electrode that is a gate material comprising a blend of carbon ink and an indicator and a Ag / AgCl reference electrode. The third layer may include a semiconducting channel formed of PEDOT:PSS. The fourth layer may include a dielectric ink. The environment sensor may be used to determine an electrical conductivity, calculate a temperature, and measure a pH of the environment.

The present invention relates to compositions for detecting a lung cancer disease, comprising peptide affinity ligands for detecting different combinations of proteins of different groups of biomarkers which include CYFRA 21-1, Prolactin, Squamous Cell Carcinoma Antigen (SCC), Her2 / Neu, Cancer antigen 125 (CA125), carcinoembryonic antigen (CEA), C- reactive protein (CRP), Neuron Specific Enolase (NSE), C4 complement (C4), Interleukin 6 (IL-6), and Metallopeptidase Inhibitor 1 (TIMP-1). The invention further relates to methods of detecting or subtyping lung diseases, as well as to computer-implemented methods based on machine learning methods using expression data of the biomarkers.

Various examples are directed to systems and methods for using analyte sensor systems such as, for example, analyte sensor and analyte sensor configured to sense a first analyte when operated in a first mode and two cents a second analyte when operated in a second mode. In some examples, a concentration of the first analyte is determined based on a temperature value generated by a temperature sensor. In some examples, an error of an analyte sensor is determined. In some examples, a baseline sensor characteristic is determined.

A method of evaluating the analytical performance of a biochemical measured using a multi-analyte assay is described. The method includes measuring a value for the level of a first biochemical in an analytical verification sample, wherein the first biochemical has been previously analytically verified against three or more analytical verification conditions; measuring the level of a second biochemical in a sample, wherein the second biochemical is structurally or biochemically related to the first biochemical; and comparing a verification parameter for the first biochemical and a verification parameter for the second biochemical to determine whether the performance of the second biochemical is acceptable based on the comparison.

Detection reagents, multi-analyte test elements, test systems, and multi-analyte measuring methods are provided. In particular, multi-analyte test elements have (1) a first working electrode and first counter electrode pair covered with a first analyte-specific reagent that includes an enzyme, a coenzyme and a first mediator and have (2) a second working electrode covered with a second analyte-specific reagent that includes an enzyme, a coenzyme and a second mediator, where the second mediator is different than the first mediator. The single counter electrode can be used as the counter electrode for both the first and second analyte measurements at their respective working electrodes. Moreover, the mediator concentrations, measurement ranges, and applied potential differences are not the same for each analyte-specific measurement.

Provided herein are digital and graphical user interfaces for multi-analyte monitoring systems. For example, disclosed herein are various embodiments of methods, systems, and interfaces related to alarm interfaces, alarm setting interfaces, alarm disabling interfaces and functions, and sensor result interfaces. Also disclosed herein are various embodiments of interfaces related to glucose and ketone body sensing.

The technology relates in part to multi-analyte rapid tests. In certain aspects, the technology relates in part to interchangeable multi-analyte rapid tests. In certain aspects, the technology relates in part to enzyme-based multi-analyte rapid tests. In certain aspects, the technology relates to enzyme-immobilized-based multi-analyte rapid tests. In certain aspects, the technology relates to interchangeable multi-analyte rapid tests. In certain aspects, the technology relates to enzyme-based interchangeable multi-analyte rapid tests. In certain aspects, the technology relates to enzyme-based multi-analyte lateral flow rapid tests. In certain aspects, the technology relates to enzyme-immobilized-based interchangeable multi-analyte lateral flow rapid tests.

Provided herein are systems, devices and methods for performing multianalyte detection in a biological sample, such as a human blood sample. Multiwell plates useful for performing multianalyte detection are also provided. The systems, devices and methods provided herein relate to the field of direct-to-consumer diagnostics (DTC diagnostics) and are useful, e.g., for facilitating consumer access to consumer healthcare and consumer wellness information. Other uses of the systems, devices and methods provided herein relate to the fields of medical research and drug discovery.

Single-use diagnostic consumables for use in performing multiple analyses on a fluid sample are provided. The diagnostic consumables include a first sensing region configured for analysis of at least one analyte in a fluid sample that has been received by the diagnostic consumable. The diagnostic consumable further includes a fluid transport material configured to flow a portion of the fluid sample into a second sensing region fluidically connected to the fluid transport material and configured for performing a second analysis of the fluid sample. Methods for performing multiple analyses of a fluid sample on a single-use diagnostic consumable are also provided.