The invention belongs to the technical field of microbiological detection, and particularly relates to a closed
nucleic acid extraction, amplification and detection fully-integrated device. Comprising an
injector type
nucleic acid extraction, enrichment and amplification
assembly, a detection card box, a three-way valve and a sealing cover, wherein the
nucleic acid extraction, enrichment and amplification
assembly comprises a
chitosan modified
paper sheet used for nucleic acid enrichment and an
injector, the
chitosan modified
paper sheet is fixed in a needle cylinder of the
injector through a rubber plug, and a
piston of the injector is isolated from the
chitosan modified
paper sheet through isolation liquid; the detection card box is internally provided with at least one test strip, a box body is provided with a vent hole, the vent hole is covered with a film, and the film is breathable and waterproof; when an amplification product is loaded, one end of the three-way valve is connected with the detection card box, the other end of the three-way valve is connected with the nucleic acid extraction, enrichment and amplification
assembly, and the third end of the three-way valve serves as a liquid inlet; during detection, the sealing cover is connected with the third end of the three-way valve.