This invention relates to the fields of analytical detection and online
solid-phase extraction technology, specifically to a
rapid detection method for trace
plasma samples used in pharmacokinetic studies. The method first measures the empty
baseline pressure; after injection, it calculates the net sample pressure and
total resistance integral in real time, and identifies the
transition point from injection to cleaning to lock the fluid dynamics boundary parameters; during the cleaning phase, it calculates the measured
pressure decay rate and extrapolates the theoretical
pressure decay rate under ideal emptying conditions, uses the
total resistance integral to compensate for
viscosity at the measured rate, and then obtains the flow
hysteresis ratio characterizing the sample matrix retention effect based on both; finally, it uses the flow
hysteresis ratio to adaptively correct a standard threshold to obtain a target threshold, and performs flow
path switching when the measured rate reaches the target. This invention effectively decouples
sample viscosity from adsorption characteristics, enabling adaptive adjustment of the cleaning
time based on real-time sample kinetic feedback, completely eliminating matrix residue
contamination while ensuring the
recovery rate of polar drugs.