Production of artificial hybrid lingzhi and uses thereof

A kind of Ganoderma lucidum, artificial technology, applied in the production and application field of artificial hybrid Ganoderma lucidum, can solve the problems of low seed setting rate, inability to develop nutritional mutant commercial products, hybridization failure and the like

Inactive Publication Date: 2006-03-08
赵绍惠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may reflect: disadvantages of laboratory strains, e.g., inability to develop commercial nutritional mutants, and / or low quality artificial hybrids, such as sterility, poor growth and / or low seed set, or hybrid failure

Method used

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  • Production of artificial hybrid lingzhi and uses thereof
  • Production of artificial hybrid lingzhi and uses thereof
  • Production of artificial hybrid lingzhi and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Artificial hybridization of Ganoderma lucidum and Ganoderma lucidum by protoplast fusion.

[0030] (a) The two parents are vegetative mycelia grown in a complete medium (CM) broth consisting of: MgSO 4 ·7H 2 O, 0.5; KH 2 PO 4 , 0.46; K 2 HPO 4 , 1; peptone, 2; D-glucose, 20 (g / L), cultured in the dark, at a temperature of 28° C. and a rotational speed of 120 rpm, and recorded the time. Actively growing mycelia were harvested using aseptic technique, filtered through a 1 mm diameter nickel mesh, and rinsed with infiltration buffer (0.6 M in sucrose solution). Then, the mycelia were cultured for 1 to 3 hours in permeabilization buffer containing 10 mg / ml of lysozyme (Guangdong Institute of Microbiology, China) to dissolve the fungal cell wall with the enzyme. The number of generated protoplasts was counted with a hemocytometer by phase contrast light microscopy.

[0031] After enough protoplasts were released, the undigested mycelia were filtered out with a syringe...

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Abstract

An artificial hybrid lingzhi was created by protoplast fusion between a cultivar DEG C, and was characterized by unique DNA fingerprints by random amplified polymorphic DNA markers using arbitrary primers: PP4L, PS3L, PS1L, POM and UK, and sequences of nuclear ribosomal DNA gene and mitochondrial ribosomal DNA gene different from those of the parental cultivar.

Description

Technical field: [0001] The present invention relates to the production and application of an artificial hybrid Ganoderma lucidum. The artificial hybrid Ganoderma lucidum is formed by protoplast fusion of cultivated species of Ganoderma lucidum genus and wild provenance isolates of Ganoderma lucidum. The hybrid strain of this biotechnology breeding is called " Peninsula Ganoderma - Creative No. 1". Background of the invention: [0002] Ganoderma lucidum is a group of medicinal fungi that includes Ganoderma lucidum, Ganoderma lucidum, and Ganoderma lucidum. The species cannot hybridize with each other, and the ribosomal DNA sequence and the ribosomal DNA gene sequence of the mitochondria are also different. Ganoderma lucidum grows in a wider temperature range than Pinus fir ganoderma. It has been found that these medicinal fungi can display different medicinal effects, and their biochemical composition includes polysaccharides and terpenes. Natural Ganoderma grows in diffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G1/04
Inventor 赵绍惠
Owner 赵绍惠
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