Preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles

A nanoparticle and gene technology, applied in the field of preparation of new composite nano-DNA materials, can solve the problems of large detection error, large dosage, limited controllable information, etc., and achieve the effect of good DNA stability and high measurement accuracy

A nanoparticle and gene technology, applied in the field of preparation of new composite nano-DNA materials, can solve the problems of large detection error, large dosage, limited controllable information, etc., and achieve the effect of good DNA stability and high measurement accuracy

CN107699609AInactive Publication Date: 2018-02-16张毅
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  • Preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles
  • Preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles
  • Preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Add 2g Fe 3 o 4 Nanoparticles were dispersed into 500mL of mixed solvent with alcohol-water ratio of 5:1;

[0042] 2) Add 10 mL of 50% TMAPS (in methanol) under stirring condition, and wash with ethanol for 3 times after reacting for 8 hours;

[0043] 3) redispersing the obtained nanoparticles into water, adding DNA of a specific sequence, the DNA is a DNA gene fragment longer than 20 base pairs, stirring and reacting for 8 hours, separating and washing to obtain surface DNA-modified nanoparticles;

[0044] 4) Disperse the nanoparticles loaded with DNA in 95% ethanol;

[0045] 5) Add TMAPS and TOES to generate SiO on the surface of nanoparticles 2 layer that protects DNA. SiO 2 The thickness of the layer can be tuned by changing the amount of TMAPS and TEOS.

[0046] The resulting gene tracer and anti-counterfeiting nanoparticles such as figure 1 shown.

Embodiment 2

[0048] 1) Add 2g of Cr 2 o 3 Nanoparticles were dispersed into 500mL of mixed solvent with alcohol-water ratio of 5:1;

[0049] 2) Add 10 mL of 50% TMAPS (in methanol) under stirring condition, and wash with ethanol for 3 times after reacting for 8 hours;

[0050] 3) redispersing the obtained nanoparticles into water, adding DNA of a specific sequence, the DNA is a DNA gene fragment longer than 20 base pairs, stirring and reacting for 8 hours, separating and washing to obtain surface DNA-modified nanoparticles;

[0051] 4) Disperse the nanoparticles loaded with DNA in 95% ethanol;

[0052] 5) Add TMAPS and TOES to generate SiO on the surface of nanoparticles 2 layer that protects DNA. SiO 2 The thickness of the layer can be tuned by changing the amount of TMAPS and TEOS.

[0053] The resulting gene tracer and anti-counterfeiting nanoparticles such as figure 2 shown.

Embodiment 3

[0055] 1) Add 2g of CaCO 3 Nanoparticles were dispersed into 500mL of mixed solvent with alcohol-water ratio of 5:1;

[0056] 2) Add 10 mL of 50% TMAPS (in methanol) under stirring condition, and wash with ethanol for 3 times after reacting for 8 hours;

[0057] 3) redispersing the obtained nanoparticles into water, adding DNA of a specific sequence, the DNA is a DNA gene fragment longer than 20 base pairs, stirring and reacting for 8 hours, separating and washing to obtain surface DNA-modified nanoparticles;

[0058] 4) Disperse the nanoparticles loaded with DNA in 95% ethanol;

[0059] 5) Add TMAPS and TOES to generate SiO on the surface of nanoparticles 2 layer that protects DNA. SiO 2 The thickness of the layer can be tuned by changing the amount of TMAPS and TEOS.

[0060] The resulting genetic tracer and anti-counterfeiting nanoparticles such as image 3 shown.

[0061] Of course, you can also use C, Fe 2 o 3 、TiO 2 , ZnO, CdS, ZnS, MoS 2 、WS 2 Nanoparticles ...

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Abstract

The invention discloses a preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles. The method includes the steps of: a) dispersing a nano-material in a solvent, adding TMAPS and carrying out reaction for 1-24h to obtain surface positively charged nanoparticles; b) adding DNA and carrying out reaction for 24h to obtain DNA modified nanoparticles, whereinthe DNA is a DNA gene fragment longer than 20 base pairs; c) adding TMAPS and TEOS and carrying out reaction for 1-21d to form an SiO2 shell layer on the surfaces of the DNA modified nanoparticles, thus obtaining SiO2 coated nanoparticles; and d) riveting APTES to the surfaces of nanoparticles carrying genetic information so as to obtain gene tracing and anti-counterfeit nanoparticles. The methodprovided by the invention has low operation temperature, the product can exist in a temperature ranging from room temperature to 175DEG C, also can be dispersed in an aqueous phase or oil phase, hasthe advantages of high gene detection sensitivity, no pollution, good stability, high detection precision, multi-point continuous monitoring in a wide range and the like, and can be widely applied inanti-counterfeiting and tracing and other fields.

Description

technical field [0001] The invention relates to a preparation method of a novel composite nano-DNA material, in particular to a preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles. Background technique [0002] DNA is a key biopolymer material that carries a large amount of information. Natural DNA determines the diversity of species, while synthetic DNA has unlimited possibilities in terms of species. As a key biomolecule, DNA has been widely used in different fields and plays an extremely important role in modern science and technology. Among them, the new materials designed based on the large amount of information carried by DNA have incomparable exclusivity or uniqueness. For example: [0003] (1) In the field of commodity anti-counterfeiting protection, the principle that a specific DNA fragment has a specific coding structure is used to make the anti-counterfeiting label contain a certain DNA and can be preserved for a...

Claims

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Application Information

Patent Timeline
16 Feb 2018
Publication
CN107699609A
IPC
C12Q1/6851; C12Q1/6869; B22F1/00; B82Y5/00
CPC
C12Q1/6851; C12Q1/6869; B82Y5/00; B22F1/054; C12Q2563/155; C12Q2531/113; C12Q2561/113
Inventors
张毅