Method for detecting characteristics of gene region based on inter-alu polymerase chain reaction

A chain reaction and polymerase technology, applied in the biological field, can solve problems such as the lack of in-depth research on the genome
CN101792808AInactive Publication Date: 2010-08-04GUANGZHOU HKUST FOK YING TUNG RES INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU HKUST FOK YING TUNG RES INST
Publication Date
2010-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for centralized expanding and detecting the gene region of a genome DNA, which aims to verify the characteristics of the gene area in the genome. The characteristics comprise single nucleotide polymorphism (SNP), point mutuation, sequence insertion / deletion and the levels of dideoxy nucleotide (DNA) methylated CpG sites. With an Alu family, particularly an AluY subfamily common sequence as the main oligonucleotide primer, the method expands the DNA and copies all gene regions which are mostly concentrated in the genome with the oligonucleotide primer inter-alu PCR expanded genome DNA. The method is characterized in that the inter-Alu PCR can filter some non-gene sequences, so that a novel generation of sequencing technology detects the SNP, the point mutuation, the sequence insertion / deletion and the levels of DNA methylated CpG sites of the gene region in a centralized way, so as to save the consumption of the genome DNA required by the sequencing.
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Description

technical field

[0001] The invention belongs to the field of biotechnology. Specifically, it is a method involving concentrated detection of single nucleotide polymorphism (SNP), point mutation, sequence insertion / deletion and dideoxynucleotide molecule (DNA) methylation CpG site. In this method, the common sequence of the Alu family, especially the AluY subfamily, is used as the main oligonucleotide primer, and the genomic DNA is amplified by inter-Alu PCR with multiple primers. Mostly concentrated in each gene region in the genome. The method is characterized in that part of the non-gene region sequence can be filtered out by inter-Alu PCR, so that the next-generation sequencing technology can concentrate on detecting SNP, point mutation, sequence insertion / deletion and DNA methylation CpG site in the gene region of the genome, And it can save the amount of genomic DNA required for sequencing. Background technique

[0002] A high-throughput sequencer designed on the bas...

Claims

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