50 results about "Sequence Insertions" patented technology

A method for providing secure transactions generates a Secure Card Number (“SCN”) for a first entity that is transferred with a first entity identifier to a second entity and then to a money source that verifies that the transaction is valid by use of the first entity identifier and the SCN. The SCN includes a Transaction Information Block (“TIB”), a Counter Block, and an encrypted Personal Identification Number (“PIN”) Block. The SCN is transferred to the money source in an account number or a non-account data field. The money source can use the TIB to determine whether the SCN should be used once or multiple times or to identify one of several physical devices, all of which are issued to the first entity, used to generate the SCN. The money source validates the SCN by duplicating the encryption process used to create an encrypted PIN Block and comparing the result to the encrypted PIN Block received with the transaction. A Triple Data Encryption Standard algorithm encrypts a PIN Block generated from a PIN, a Sequence Insertion Number (“SIN”) and a known starting value. The SIN can be a combination of three seed values and a random value generated by a Pseudo Random Number Generator (“PRNG”) initialized with the seed values. A Counter value is associated with the Counter Block and the seed values.

The construction of a chimeric Pestivirus by the identification of selected regions in the 3′NTR of the viral RNA genome is described where additional RNA sequences can be stably inserted. These sequence insertions in the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.

The invention discloses a 9B / 10B encoding method, which is used for encoding a 9-bit source sequence set into a 10-bit target sequence set. The 9-bit source sequence set comprises a first type of 9-bit source sequence set, a second type of 9-bit source sequence set and a third type of 9-bit source sequence set. The first type of 9-bit source sequence set only contains 9-bit source sequences with difference values of 1 and-1, and after 1-bit data 0 or 1 is inserted into the same position of each sequence in the first type of 9-bit source sequence set, the sequence is encoded into a 10-bit target balance sequence; the second type of 9-bit source sequence set selectively only contains 9-bit source sequences with the difference value of 3 or only contains 9-bit source sequences with the difference value of-3, 1-bit data 0 or 1 is inserted into each sequence in the second type of 9-bit source sequence set, and the sequence is encoded into a pair of 10-bit target unbalanced sequences with the difference value of + / -2; and each sequence in the third type of 9-bit source sequence set is encoded into a pair of 10-bit target unbalanced sequences which are opposite of each other.

The invention discloses a 9B / 10B coding method suitable for low-pass and band-pass channels, which is used for coding a 9-bit source sequence set into a 10-bit target sequence set, and the 10-bit target sequence set does not contain balance sequences' 1010101010 'and' 0101010101 '. The 9-bit source sequence set comprises a first type of 9-bit source sequence set, a second type of 9-bit source sequence set and a third type of 9-bit source sequence set. The first type of 9-bit source sequence set only contains 9-bit source sequences with difference values of 1 and-1, and after one-bit data '0' or '1' is inserted into the same position of each sequence in the first type of 9-bit source sequence set, the sequence is coded into a 10-bit target balance sequence; the second type of 9-bit source sequence set selectively only contains 9-bit source sequences with the difference value of 3 or only contains 9-bit source sequences with the difference value of-3, one bit of data '0' or '1' is inserted into each sequence in the second type of 9-bit source sequence set, and a pair of 10-bit target unbalanced sequences with the difference value of + / -2 is coded.

The present invention discloses a method for determining the sequence, the insertion position and the boundary sequence of an exogenous DNA fragment in a transgenic organism. The method comprises: sequencing the whole genome of a transgenic organism, comparing a sequence A to the whole genome sequence of the transgenic organism, and determining the sequence, the insertion position and the boundarysequence of the exogenous DNA fragment in the transgenic organism, wherein an acceptor organism is treated by a vector containing the exogenous DNA fragment to obtain the transgenic organism, and thesequence A is the following a1), a2) or a3): a1) the full-length sequence of the vector, a2) the sequence of any one DNA fragment derived from the vector and containing the exogenous DNA fragment, and a3) the sequence of the exogenous DNA fragment or any one fragment thereof. According to the present invention, the method can break through the limitation in species, is not limited by the target gene for constructing the transgenic organism, and can rapidly and precisely detect the insertion position, the boundary sequence and even the copy number of the transgenic gene.