8 results about "Sequence Insertions" patented technology

Provided is an efficient and precise plant gene knockout method. First, a sequence containing a stop codon cluster is inserted into a genome by means of dual pegRNA to obtain, by means of screening, a stop codon cluster sequence capable of efficiently and precisely knocking out a target gene. A prime editing system and multiple pegRNA programming can be used in combination with the method to enable various genomic modifications such as efficient and precise knockout of one or more target genes, base substitutions, and small fragment insertions, deletions, and substitutions with just one prime editing protein.

The application discloses a method for identifying large fragment sequence insertion of a target genomic region and application thereof, and belongs to the technical field of bioinformatics. In view of the problem that large fragment insertion variation is difficult to be accurately recognized in clinical metagenomic sequencing due to short sequencing read length, insufficient coverage and other factors, the application proposes to construct a reference sequence which can represent the insertion variation by means of manual construction, and to realize efficient identification of the insertion event of the target genomic region by combining a short read-based fast alignment process. The method overcomes the dependence of existing structural variation detection tools on high sequencing depth and long read length, has the advantages of fast identification speed, high sensitivity and high accuracy, and is suitable for rapid screening of large fragment insertion related to drug resistance mechanism in clinical samples. Meanwhile, the method can be popularized for insertion variation analysis of other pathogen drug resistance related genes or genomic regions, and has a good clinical application prospect.

The present invention provides, among other things, methods of engineering a re-editable target locus in a cell, comprising inserting an exogenous re-editable template sequence to a target locus and methods of engineering a re-editable cell comprising a re-editable template sequence flanked by two homology arms such that the re-editable template sequence is inserted into a target locus by homologous recombination, and methods of using the same. In some aspects, provided is a re-editable template sequence, wherein the template sequence has no substantial sequence similarity to any region in the genome, and wherein the template sequence is recognizable by a genome editing system.

The invention discloses a method for identifying large-fragment sequence insertion in a target genome region and application of the method, and belongs to the technical field of bioinformatics. In order to solve the problem that large-fragment insertion variation is difficult to accurately identify due to factors such as short sequencing reading length and insufficient coverage in clinical metagenome sequencing, the invention proposes that a reference sequence capable of representing the insertion variation is artificially constructed and is combined with a rapid comparison process based on short reads to realize efficient identification of an insertion event in a target genome region. The method overcomes the dependence of an existing structure variation detection tool on high sequencing depth and long reading length, has the advantages of high identification speed, high sensitivity, high accuracy and the like, and is suitable for rapid screening of large fragment insertion related to a drug resistance mechanism in a clinical sample; meanwhile, the method can be popularized and applied to insertion variation analysis of other pathogen drug resistance related genes or genome areas, and has a good clinical application prospect.

Embodiments of the present disclosure relate to a method, apparatus, device, storage medium and program product for data query. The method comprises: determining, based on relevance to query information, a relevant data set from candidate data sets, the relevant data set comprising a plurality of relevant data, the relevance of the plurality of relevant data to the query information exceeding a threshold; adjusting, based on a preset sequence insertion operation, an arrangement order of the plurality of relevant data in the relevant data set; determining, based on the adjusted arrangement order of the plurality of relevant data, a prompt word having a preset format, the prompt word having the preset format being at least associated with an insertion mode of the preset sequence insertion operation; and obtaining a query result for the query information by inputting the prompt word and the relevant data associated with the prompt word into a preset query model. Thus, the accuracy of the search result can be improved.

The present invention provides, in particular, a method for recombining a re-editable target locus within a cell, comprising inserting an exogenous re-editable template sequence into the target locus; a method for recombining a re-editable cell, comprising a re-editable template sequence having two adjacent homology arms, wherein the re-editable template sequence is inserted into the target locus by homologous recombination; and methods for using the same. In some embodiments, a re-editable template sequence is provided, which has virtually no sequence similarity to any region in the genome, and which is recognizable by a genome editing system.

The present disclosure provides compositions and methods useful for inserting an exogenous donor DNA fragment into a target genomic sequence in a cell. The method comprises introducing two extension arms on a target genomic sequence and presenting to the genomic sequence a donor DNA having two complementary fragments flanking the donor fragment. The pairing of the extension arms allows donor DNA to be integrated into the genomic sequence. Depending on the configuration of the extension arm and a complementary fragment on the donor DNA, a portion of the genomic sequence may be repeated or deleted during this process.

The invention belongs to the field of gene engineering, and discloses an efficient and accurate plant gene knockout method. The method comprises the following steps: firstly, inserting a sequence containing a termination codon cluster into a genome through double pegRNA, so as to screen a termination codon cluster sequence capable of efficiently and accurately knocking out a target gene; in combination with the method, a guide editing system and a plurality of pegRNA programs are utilized, and various genome modifications such as efficient and accurate knockout, base replacement, small fragment insertion, deletion and replacement of one or more target genes can be realized by only one guide editing protein.