50 results about "Sequence Insertions" patented technology

A method for providing secure transactions generates a Secure Card Number (“SCN”) for a first entity that is transferred with a first entity identifier to a second entity and then to a money source that verifies that the transaction is valid by use of the first entity identifier and the SCN. The SCN includes a 0Transaction Information Block (“TIB”), a Counter Block, and an encrypted Personal Identification Number (“PIN”) Block. The SCN is transferred to the money source in an account number or a non-account data field. The money source can use the TIB to determine whether the SCN should be used once or multiple times or to identify one of several physical devices, all of which are issued to the first entity, used to generate the SCN. The money source validates the SCN by duplicating the encryption process used to create an encrypted PIN Block and comparing the result to the encrypted PIN Block received with the transaction. A Triple Data Encryption Standard algorithm encrypts a PIN Block generated from a PIN, a Sequence Insertion Number (“SIN”) and a known starting value. The SIN can be a combination of three seed values and a random value generated by a Pseudo Random Number Generator (“PRNG”) initialized with the seed values. A Counter value is associated with the Counter Block and the seed values.

The invention relates to a conditional gene knockout method constructed by using a CRISPR/Cas9 system. LoxP-antibiotic-LoxP and FRT-antibiotic-FRT-LoxP sequence insertion sites are selected from leftand right sides of a to-be-knocked-out area respectively; left and right homologous arms are selected from upstream and downstream positions of to-be-inserted sites, amplification, purification, enzyme digestion, linking and conversion are performed, and left and right targeting plasmids are obtained; Cas9 plasmid, the left or right targeting plasmid of the knockout area and corresponding gRNA expression plasmids are sequentially or simultaneously introduced into cells, screening is performed with antibiotics after transfection, and target cells with left and right targeting sequences insertedare screened out simultaneously; 4-OHT and DOX are used for inducing Cre-LoxP and FLP-FRT medicated homologous recombination, so that loxp sites are introduced in left and right sides of the to-be-knocked-out area; 4-OHT is used for inducing Cre-LoxP medicated homologous recombination, and cloning of target fragment knockout is obtained.

An A/D converter stage including an A/D sub-converter connected to a D/A sub-converter (12) is calibrated by a method that inserts a calibration test sequence into the D/A sub-converter. This is accomplished by forcing (SW) the comparators (COMP1-COMP7) of the A/D sub-converter to generate and insert the sequence into the D/A sub-converter.

The invention discloses a gene editing technique taking Argonaute nuclease as a core. In the presence of guide DNA (Deoxyribose Nucleic Acid), Argonaute endonuclease can cut any locus of a targeted DNA sequence, including a genome of a mammal to cause breakage of DNA double chains, thereby fulfilling an editing aim. Editing means include incision, deletion, mutagenesis induction, exogenous sequence insertion, fragment substitution and the like. Editing effects include gene inactivation, gene mutation, exogenous gene induction and the like. The protein can serve as an important tool for genome editing. A gene editing tool taking the protein as the core has the characteristics of easiness in operation, high efficiency, low target missing rate and high fidelity. Moreover, almost any genome locus can be effectively targeted and cut by the Argonaute nuclease. By using the technique, high-specificity and high-efficiency genome targeted editing is helpfully realized.

The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.

The invention discloses an encoding and decoding method for improving decoding efficiency by pilot frequency information and a device thereof. The method comprises the following steps that: pilot frequency sequences are evenly inserted into an information sequence before encoding, and the information sequence is subjected to error correction encoding by an error correcting code; the encoded information sequence is generated into an OFDMA symbol after IFFT conversion, and is transmitted to a receiving end; the receiving end carries out FFT conversion on the received information sequence to obtain soft information corresponding to the information sequence which is encoded by a transmitting end; the positions of the pilot frequency sequences inserted into the information sequence are set into corresponding soft information values according to values of the pilot frequency sequences; and the information is subjected to error correcting decoding to obtain transmitting information of the transmitting end. The method and the device can improve the velocity of convergence and decoding performance of an error correcting decoder, reduce the times of decoding iteration, reduce decoding time delay, and simplify the process of decoding.

The invention provides a method for detecting expansion of a short tandem repeat. The method comprises the following steps: firstly, carrying out sequence alignment; secondly, detecting short tandem repeat of third-generation sequencing data by RepeatHMM; thirdly, detecting sequence insertion of a short tandem repeat area by inScan; fourthly, calculating an intersection of RepeatHMM detection results and sequence insertion detection results of the short tandem repeat area. According to the method provided by the invention, the results of the sequence insertion and RepeatHMM short tandem repeatdetection are combined, so that the specificity of detecting the expansion of the short tandem repeat is improved.

The invention discloses a preparation method and an application for a dual-fluorescence reporting system for detecting a micro-RNA (ribonucleic acid) function, wherein the preparation method comprises the following preparation steps of: A, obtaining a mCherry gene sequence via a PCR (polymerase chain reaction) amplification, inserting a rho EGFP-C1 carrier to replace the EGFP (enhanced green fluorescent protein) sequence of the mCherry gene sequence, and constructing a carrier rho mCherry-C1; B, using a RNAi-Ready rho SIREN-RetroQ plasmid as a template, obtaining a human U6 promoter gene via a PCR amplification, and inserting the carrier rho mCherry-C1 to obtain a plasmid rho hU6-mCherry-C1; and C, obtaining a gene sequence carried with a CMV (cytomegalovirus) promoter and an EGFP expression dialog sequence in an interval of 358 to 1944 on a plasmid rho Adtrack-CMV, and inserting the sequence in the plasmid rho hU6-mCherry-C1 to obtain a plasmid rho MGhU6. The plasmid rho MGhU6 is a dual-fluorescence reporting system simultaneously containing a mCherry reporting gene, an internal reference EGFP, a micro-RNA and the target sequence insertion site thereof. The reporting system can be used for detecting the inhibition function of the micro-RNA to the target sequence expression. The detection method is easy, as well as simple and convenient in operation; and a function detection for the micro-RNA can be realized by transfecting one plasmid only. The system also can be used for visually detecting the inhibition function of a micro-RNA in a single living cell to the target thereof by the aid of fluorescence microscopic imaging.

The invention belongs to the technical field of genome sequencing, and discloses an insertion mutation detection method based on new-generation sequencing data. The method comprises the steps: when amutation generation site is determined, a region where insertion mutation occurs certainly generates a split reading section, aiming at the characteristics that insertion variation types such as new sequence insertion, sequence series multiplication and sequence dispersion multiplication are different in distribution of missing variation and inverted mutation split reading sections, constructing avirtual reference sequence by utilizing partial matching, complete matching and unmatched read segment information after determining the insertion mutation generation type and site, and comparing thevirtual reference sequence with the original reference sequence to obtain related information of the insertion sequence; and obtaining a mutant genotype by utilizing the copy number state information. According to the invention, the problem of inaccurate insertion mutation point judgment can be solved; the problem of omission caused by insertion mutation detection of an SR method can be solved; the problem that in the prior art, repeated sequences may cause detection errors can be solved.

The invention discloses a polypeptide, a lipoprotein-like nano particle (LNP) and application thereof. The LNP contains at least one phospholipid and at least one polypeptide. A polypeptide series helix-ring-helix structure polypeptide contains at least two amphiphilic alpha helixes, and the two amphiphilic alpha helixes are connected through at least one connecting peptide containing a cyclic structure. The helix part of the polypeptide is imbedded into a lipoprotein-like nano particle surface layer, stable formation of lipid nano particles with uniform size and relatively small particle diameters can be promoted, meanwhile cyclic sequences stretch out of the surfaces of the lipoprotein-like nano particles, and functional sequence insertion is further facilitated, so that multi-functionalization of the LNP is realized. Moreover, the application of the LNP can be further expanded by using an LNP packaged lyophobically functional substance, and for example, the LNP can be used as a drug delivery system; the drug delivery system has the characteristics of being low in toxicity and high in stability, facilitating functionalization and the like, and a simple, convenient and effective means is provided for target killing of cancer cells and cell and tissue imaging.

The invention relates to a method for centralized expanding and detecting the gene region of a genome DNA, which aims to verify the characteristics of the gene area in the genome. The characteristics comprise single nucleotide polymorphism (SNP), point mutuation, sequence insertion/deletion and the levels of dideoxy nucleotide (DNA) methylated CpG sites. With an Alu family, particularly an AluY subfamily common sequence as the main oligonucleotide primer, the method expands the DNA and copies all gene regions which are mostly concentrated in the genome with the oligonucleotide primer inter-alu PCR expanded genome DNA. The method is characterized in that the inter-Alu PCR can filter some non-gene sequences, so that a novel generation of sequencing technology detects the SNP, the point mutuation, the sequence insertion/deletion and the levels of DNA methylated CpG sites of the gene region in a centralized way, so as to save the consumption of the genome DNA required by the sequencing.

The invention provides a method for reducing the peak-to-average ratio in an orthogonal frequency division multiplexing system and includes the following steps: the input data of the orthogonal frequency division multiplexing system is converted into parallel data by a series-parallel conversion; scrambling codes are inserted, and the signal sequences with the lengths of K of the orthogonal frequency division multiplexing system are evenly divided into M blocks and the K/M is ensured to be an integer and a scrambling code is inserted into the end of each block and the total bit M of the scrambling codes are inserted; then the inverse fourier transform and series-parallel conversion are carried out rapidly; the peak-to-average ratios of signal sequences, which meet the setting requirements are calculated and output; if not meeting the requirements, scrambling codes are carried out. The method adopts a scrambling code insertion technology and can be simply realized at both sending terminal and the receiving terminal of the system without transmitting any auxiliary information. Compared with the scheme of the existing sequence insertion, the method can better reduce the peak-to-average ratio of the OFDM system on the premise of not increasing the complexity of the system.

The invention provides an adaptive cardinal number tree dynamic indexing method based on GPU parallelism. Firstly, an adaptive cardinal number tree data structure is constructed; the first two layerscreate tree nodes of the Node256 type; a cardinal number sequencing method based on high-order priority is created for the third layer and the fourth layer; tree nodes capable of accommodating corresponding sizes are created according to the number of the branches; the creation of a dynamic data structure is realized; it can be ensured that the latest update in the original batch of data is stillbehind the old update after sorting; and de-duplication is performed, redundant old updates are removed, latest updates are reserved, each section of sequence without duplicate data is inserted into anode corresponding to the section after duplicate removal to finish the establishment of the whole self-adaptive cardinal number tree, and secondly, data insertion, query and deletion operations canbe performed in parallel based on GPU parallel computing capability.

The invention provides a gene editing vector for corynebacterium glutamicum. The gene editing vector has higher gene editing efficiency. The gene editing vector comprises a Cas9 expression element, an sgRNA expression element, a replicon element and a homologous repairing sequence insertion site, wherein the Cas9 expression element comprises a resistance gene, an operon, a promoter, a SD sequence, a cas9 sequence and a terminator; the cas9 sequence is shown as SEQ ID No.5; the SD sequence is shown as SEQ ID No.6; the sgRNA expression element comprises a promoter, an sgRNA scaffold sequence and a terminator; the sgRNA scaffold sequence comprises trancrRNA and crRNA insertion sites; the sgRNA scaffold sequence is shown as SEQ ID No.7; AND the replicon element comprises a corynebacterium glutamicum replicon repA.

The invention discloses a multimedia broadcasting wireless signal anti-noise transmission method; in the multimedia broadcasting wireless signal anti-noise transmission method, the multimedia broadcasting wireless signal is at the multimedia broadcasting wireless transmitter terminal, a multimedia data stream is processed by media data processing, bit stream LDPC coding, code element modulation, constellation rotating, frame body formation, time domain training sequence insertion framing, pulse formation and up-conversion to carrier wave to form a radio-frequency signal which is transmitted to an air wireless information channel, and the signal transmitted by the multimedia broadcasting wireless transmitter terminal is received and processed by a receiver terminal; the multimedia broadcasting wireless signal anti-noise transmission method has the advantages of short receiving synchronization time, high transmission efficiency and controllable multi-service and the like.

The invention discloses a 9B/10B encoding method, which is used for encoding a 9-bit source sequence set into a 10-bit target sequence set. The 9-bit source sequence set comprises a first type of 9-bit source sequence set, a second type of 9-bit source sequence set and a third type of 9-bit source sequence set. The first type of 9-bit source sequence set only contains 9-bit source sequences with difference values of 1 and-1, and after 1-bit data 0 or 1 is inserted into the same position of each sequence in the first type of 9-bit source sequence set, the sequence is encoded into a 10-bit target balance sequence; the second type of 9-bit source sequence set selectively only contains 9-bit source sequences with the difference value of 3 or only contains 9-bit source sequences with the difference value of-3, 1-bit data 0 or 1 is inserted into each sequence in the second type of 9-bit source sequence set, and the sequence is encoded into a pair of 10-bit target unbalanced sequences with the difference value of +/-2; and each sequence in the third type of 9-bit source sequence set is encoded into a pair of 10-bit target unbalanced sequences which are opposite of each other.

The invention provides a gene editing method based on the Vcre-Vloxp recombinase system and application of the gene editing method. The gene editing method includes the following steps that (1) a Vloxp sequence is connected to the 5' tail end or 3' tail end of a double-stranded DNA, or the Vloxp sequence is inserted between the functional regions of the double-stranded DNA; and (2) the double-stranded DNA, a Vcre protein and a gene editing reaction solution are mixed, and then transferred into a target cell to complete the gene editing. When the double-stranded DNA is repeatedly connected during an integration process, the double-stranded DNA is cut under the action of the Vcre protein, finally only one double-stranded DNA is left, no matter how many double-stranded DNAs are in tandem connection, the Vcre protein can cut all repetitive fragments from the first Vloxp sequence to the last Vloxp sequence, and therefore, the gene editing method can significantly reduce the tandem duplication phenomenon of recombinant DNA fragments in the integration process.

The invention discloses a high-throughput identification method for a genome insulator. The high-throughput identification method for the genome insulator includes the steps of (1) building a reportervector, specifically, the reporter vector sequentially comprises a promoter, an ORF region, an intron, an insulator candidate sequence insertion region and an enhancer, the enhancer is used for enhancing expression of an upstream sequence, and an insulator candidate sequence is inserted in the insulator candidate sequence insertion region; (2) expressing and transcribing the reporter vector; and(3) detecting the transcribing condition of the edited transcribing sequence, and determining whether the insulator candidate sequence is the insulator or not. A reporter vector library built by the method can cover 90-99% of genome noncoding regions, protein position information is not needed, and overall analysis is achieved; the built library is transferred into a cell, whether nearly the wholegenomic sequence has insulator activity or not can be detected at the same time, large-scale parallel analysis can be carried out, and single and small-quantity analysis is avoided; and since the built library covers nearly all sites, zones of ignorance can be also detected and analyzed.

The invention discloses a 9B/10B coding method suitable for low-pass and band-pass channels, which is used for coding a 9-bit source sequence set into a 10-bit target sequence set, and the 10-bit target sequence set does not contain balance sequences' 1010101010 'and' 0101010101 '. The 9-bit source sequence set comprises a first type of 9-bit source sequence set, a second type of 9-bit source sequence set and a third type of 9-bit source sequence set. The first type of 9-bit source sequence set only contains 9-bit source sequences with difference values of 1 and-1, and after one-bit data '0' or '1' is inserted into the same position of each sequence in the first type of 9-bit source sequence set, the sequence is coded into a 10-bit target balance sequence; the second type of 9-bit source sequence set selectively only contains 9-bit source sequences with the difference value of 3 or only contains 9-bit source sequences with the difference value of-3, one bit of data '0' or '1' is inserted into each sequence in the second type of 9-bit source sequence set, and a pair of 10-bit target unbalanced sequences with the difference value of +/-2 is coded.