51 results about "Gene induction" patented technology

A biocompatible material, wherein at least a part of a surface of the biocompatible material is characterized by a micro or nano-meter scale topographical structure comprising a plurality of features where the structure is selected to promote the growth of undifferentiated pluripotent stem cells or serve to promote the uniform differentiated growth of stem cells. Furthermore, a biocompatible material is provided having a surface structure and composition that affects a cellular function, in particular cellular functions related to gene induction, cell differentiation and the formation of bone tissue in vivo and ex-vivo.

Methods and compositions relating to TIC10 are described according to aspects of the present invention. The compositions and methods have utility in treating disease, particularly cancer in a subject in need thereof, including a human subject as well as subjects of other species. The compositions have utility in treating brain cancer in a subject in need thereof.

The invention discloses a method for breeding a corn haploid induction line. An identification method for the colored embryo haploid of the induction line is developed so that haploid identification of the corn haploid induction line can be realized, thus doubling is conducted, an inducible line DH line with high inductivity, high identification efficiency and excellent agronomic traits can be screened out from the expression of four aspects of molecular marker-assisted selection inducible genes, inductivity, haploid identification efficiency and the agronomic traits, and the excellent haploidinducible line can be obtained within 1-2 years in the whole process. According to the method capable of rapidly breeding the corn haploid inducible line, the breeding process of the haploid inducible line is accelerated, a feasible scheme for solving the problems of long breeding period and large workload of the corn haploid induction line is provided, the breeding period of the haploid induction line can be greatly shortened, the workload of test of each generation is decreased, and the haploid breeding efficiency is improved, thus development of a corn haploid breeding technology is promoted, and the method has important significance.

The present invention provides modified promoters from Candida troplicalis CYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

The invention relates to a mouse anti-human CIAPIN1 monoclonal antibody for confirming multi-drug resistant gastric cancer, and a preparation method thereof. The invention is characterized in that the monoclonal antibody is obtained by adopting the hybrid tumor technology; the heavy chain is 1gG1; the light chain is k; the molecular weight is 39KD; and the affinity is 3.6x10. The preparation method comprises the following steps of: (1) synthesizing primers; (2) synthesizing single-chain DNA by carrying out reverse transcription on SGC-7901 / VCR general RNA of a human gastric-cancer multi-drug resistant cell; (3) expanding human CIAPIN1 gene cDNA by using the primers F and R and by adopting a PCR method; (4) constructing CIAPIN1 gene induction expression plasmids pET28-CIAPIN1 and pGEX-CIAPIN1; (5) constructing engineering bacteria B21-CIAPIN1 and DE3-CIAPIN1; (6) using the engineering bacteria B21-CIAPIN1 and DE3-CIAPIN1 to carry out prokaryotic expression and purification on CIAPIN1 protein; (7) using CIAPIN1 protein to immunize a 4-week-old Balb / C mouse so that mouse anti-human CIAPIN1 antibody is generated; and (8) using the serum of the immunized mouse to prepare the mouse anti-human CIAPIN1 monoclonal antibody. The invention can better identify multi-drug resistant gastric cancer and non-multi-drug resistant gastric cancer of clinical gastric cancer patients, and predict clinical prognosis of the gastric cancer patients.

The invention discloses an efficient genetic transformation method for agrobacterium-mediated sugarcane calluses, and belongs to the field of tissue culture. According to the invention, by enhancing the Agrobacterium tumefaciens Vir gene induction activation level and the bacterial liquid dip dyeing strength of sugarcane calluses, the occurrence frequency of transgenic events is greatly improved; an antioxidant is added into subsequent co-culture, screening and differential culture media to inhibit callus browning caused by agrobacterium-enhanced dip dyeing, so that the survival rate and the differentiation capacity of the dip-dyed calluses are obviously improved; a double-marker screening system with a green fluorescent protein marker EGFP gene and a herbicide glufosinate-ammonium resistance marker Bar gene at the same time is adopted, positive transgenic calluses are visually and nondestructively screened by using an EGFP marker, positive transgenic differentiated seedlings are screened by using Bar marker resistance, the accuracy of transgenic event screening is remarkably improved, and the screening efficiency is improved. And finally, the efficient genetic transformation of the agrobacterium tumefaciens mediated sugarcane callus is realized.

The invention provides a building method of a mice spontaneous glioma immunotherapy animal model. The method is characterized by building a rapid and spontaneous glioma model by using an SB vector local transgenic technology and inducing C57 / BL6 mice to generate spontaneous glioma by using an AID gene. SB is a system comprising two parts; the two parts comprise transposons DNA and transposase. AID is an enzyme capable of inducing DNA mutation in human genome under the physiological status and is capable of deaminizing cytosine on DNA and transforming cytosine into thymine. The spontaneous glioma model is capable of monitoring the growth of the tumor by using the bioluminescence technique; the speed of inducing the generation of the tumor of the spontaneous glioma model is equal to or superior to the virus-induced animal model; meanwhile, the mice spontaneous glioma immunotherapy animal model is free of disadvantages of long virus vector preparation time, high price and low tumorigenesis rate.

The invention discloses a method for breeding maize haploid inducer lines. By developing a method for identifying colored embryo haploids of the inducer lines, the haploid identification of the maize haploid inducer lines can be realized, thereby doubling, and The induction line DH line with high induction rate, high identification efficiency and excellent agronomic traits can be screened from four aspects of molecular marker-assisted selection: induction gene, induction rate, haploid identification efficiency and agronomic traits. The overall process takes 1 ‑2 years to obtain an excellent haploid induction line. The method created by the present invention, which can be used for rapid selection of haploid induction lines in maize, accelerates the breeding process of haploid induction lines, and provides a solution to the problems of long breeding cycle and heavy workload of haploid induction lines in maize. A feasible plan can greatly shorten the breeding period of haploid induction lines, reduce the testing workload of each generation, improve the efficiency of haploid breeding, and thus promote the development of maize haploid breeding technology, which is of great significance.

The invention relates to an accelerator and a preparation method thereof, belonging to the technical field of animal husbandry biological products; by weight percentage, it comprises: 82% corn steep liquor, 6% urea, 5% mannitol, 4% DL-methionine, and L-arginine 3%; a kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4 %; B. mixed and stirred in a dry powder mixer for 60 minutes to obtain the finished product; a kind of accelerator of 5-aminolevulinic acid comprises by weight percentage: lactose 12%, triammonium citrate 3.5%, L-asparagine 14%, hemoglobin 1.5%, L-serine 69%; the accelerator provided by the invention can increase the content of 5-aminolevulinic acid in the photosynthetic bacteria fermentation broth, and has the advantages of simple production process, easy operation, no transgene and gene induction It has the advantages of low energy consumption, easy access to raw materials, high yield, low cost, convenient use, and more obvious effects.

The invention provides an expression method of recombinant human insulin, a special expression vector, engineering bacteria and application. This expression method can induce the expression of recombinant human insulin gene in food-grade lactic acid bacteria and display recombinant human insulin on the surface of lactic acid bacteria. The engineering bacteria, expression vector and inducer used in this expression method all meet the requirements of food grade, avoiding the The presence of resistance genes on vectors and the potential harm caused by non-food-grade inducers, etc. The lactic acid bacteria engineering bacteria containing cell wall display insulin obtained by the present invention can stimulate NOD mice to produce recombinant human insulin-specific antibodies, significantly increase the level of cytokine IL-4 related to immune tolerance, and induce immune tolerance. The induced lactic acid bacteria engineered bacteria can be used as an oral vaccine for type 1 diabetes after being made into bacterial preparations, without the need for tedious post-processing such as purification, and has broad application prospects.

Visual self-sucking method for inducing external gene into insect eggs is carried out by: under telescope, coating solution with external genes on insect eggs, pricking eggs to make external gene into eggs, making external genes combined with insect's gene to form transgenic insects. It makes technicians to do researches on transgenic insects with cheap instrument.

The present invention relates to the protective effect and application of FSTL1 in the homeostasis regulation of tissues such as liver against fibrosis, in particular to the application of FSTL1 protein or FSTL1 regulating compound in the preparation of medicine for treating tissue fibrosis. The invention also relates to a conditional FSTL1 gene knockout non-human animal model closely related to the degree of tissue fibrosis and its preparation method, and its use in screening anti-tissue fibrosis drugs. Among them, by combining different doses of tamoxifen and different stages of animal growth, a non-human animal model of systemic or vascular endothelial cell FSTL1 gene-induced knockout is obtained to simulate different tissue fibrosis lesions.