53 results about "Gene induction" patented technology

Methods and compositions relating to TIC10 are described according to aspects of the present invention. The compositions and methods have utility in treating disease, particularly cancer in a subject in need thereof, including a human subject as well as subjects of other species. The compositions have utility in treating brain cancer in a subject in need thereof.

A biocompatible material, wherein at least a part of a surface of the biocompatible material is characterized by a micro or nano-meter scale topographical structure comprising a plurality of features where the structure is selected to promote the growth of undifferentiated pluripotent stem cells or serve to promote the uniform differentiated growth of stem cells. Furthermore, a biocompatible material is provided having a surface structure and composition that affects a cellular function, in particular cellular functions related to gene induction, cell differentiation and the formation of bone tissue in vivo and ex-vivo.

Silicatein is an enzyme of silicate-forming organisms used for the synthesis of their silicate scaffold. The present invention relates to the use of highly-expressed and highly active recombinant silicatein, silicatein isolated from natural sources after gene induction as well as silicatein-fusion proteins for the synthesis of amorphous silicon dioxide (silicic acids and silicates), siloxanes as well as modification of these compounds and their technical use.

The invention discloses a gene editing technique taking Argonaute nuclease as a core. In the presence of guide DNA (Deoxyribose Nucleic Acid), Argonaute endonuclease can cut any locus of a targeted DNA sequence, including a genome of a mammal to cause breakage of DNA double chains, thereby fulfilling an editing aim. Editing means include incision, deletion, mutagenesis induction, exogenous sequence insertion, fragment substitution and the like. Editing effects include gene inactivation, gene mutation, exogenous gene induction and the like. The protein can serve as an important tool for genome editing. A gene editing tool taking the protein as the core has the characteristics of easiness in operation, high efficiency, low target missing rate and high fidelity. Moreover, almost any genome locus can be effectively targeted and cut by the Argonaute nuclease. By using the technique, high-specificity and high-efficiency genome targeted editing is helpfully realized.

The invention in particular relates to a method for the quantification of in vivo RNA from a biological sample comprising the steps of: collecting said biological sample in a tube comprising a compound inhibiting RNA degradation and / or gene induction; forming a precipate comprising nucleic acids; separating said precipate from the supernatant; dissolving said precipitate using a buffer, forming a suspension; isolating nucleic acids from said suspension using an automated device; dispersing / distributing a reagent mix for RT-PCR using an automated device; dispersing / distributing the isolated nucleic acids within the dispersed reagent mix using an automated device, and determining the in vivo levels of transcripts using the nucleic acid / RT-PCR reagent mix in an automated setup. The present invention also relates to the quantitatification of DNA from a biological sample. The present invention further elucidates a kit for isolating quantifiable nucleic acids from a biological sample. Applications of the method according to present invention are aldo disclosed.

The invention discloses a method for breeding a corn haploid induction line. An identification method for the colored embryo haploid of the induction line is developed so that haploid identification of the corn haploid induction line can be realized, thus doubling is conducted, an inducible line DH line with high inductivity, high identification efficiency and excellent agronomic traits can be screened out from the expression of four aspects of molecular marker-assisted selection inducible genes, inductivity, haploid identification efficiency and the agronomic traits, and the excellent haploidinducible line can be obtained within 1-2 years in the whole process. According to the method capable of rapidly breeding the corn haploid inducible line, the breeding process of the haploid inducible line is accelerated, a feasible scheme for solving the problems of long breeding period and large workload of the corn haploid induction line is provided, the breeding period of the haploid induction line can be greatly shortened, the workload of test of each generation is decreased, and the haploid breeding efficiency is improved, thus development of a corn haploid breeding technology is promoted, and the method has important significance.

Methods of activating a signaling cascade comprising, introducing leptin and / or a cytokine to a receptor complex comprising gp 130, optionally in combination with a compound acting on adenylate cyclase or acting on one or more downstream targets of adenylate cyclase, thereby inducing genes in neuro-endocrine cells or cells of neuro-endocrine origin. Two distinct gene-sets are induced, immediate early response genes (STAT-3, SOCS-3, Metallothionein-II, the serine / threonine kinase Fnk and the rat homologue of MRF-1), and late induced target genes (Pancreatitis Associated Protein I, Squalene Epoxidase, Uridinediphosphate Glucuronyl Transferase and Annexin VIII). Strong co-stimulation with the adenylate cyclase activator forskolin was shown with respect to late induced target genes. Transcripts encoding Leptin Induced Protein I (LIP-I) and Leptin Induced Protein II (LIP-II) were identified; however, no forskolin co-stimulatory effect was observed. It is also demonstrated that leptin modulates in vivo expression of MT-II, Fnk and Pancreatitis Associated Protein I genes.

The present invention provides modified promoters from Candida troplicalis CYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

The invention aims to provide an inducible promoter, a gene induced expression control system based on the inducible promoter, and an improved microbial genetic operation tool developed based on the expression control system. L-Arabinose is used as an inducing agent. Arabinose is a carbon source common in the nature and free of inhibitory action on cell growth. The induced expression system enables gene expression level to be up-regulated up to 800 times, is better in preciseness and can be used in controlled expression of target genes in Fusiformis or other microbial cells and to optimize existing genetic modification technology and develop new tools.

The invention relates to a mouse anti-human CIAPIN1 monoclonal antibody for confirming multi-drug resistant gastric cancer, and a preparation method thereof. The invention is characterized in that the monoclonal antibody is obtained by adopting the hybrid tumor technology; the heavy chain is 1gG1; the light chain is k; the molecular weight is 39KD; and the affinity is 3.6x10. The preparation method comprises the following steps of: (1) synthesizing primers; (2) synthesizing single-chain DNA by carrying out reverse transcription on SGC-7901 / VCR general RNA of a human gastric-cancer multi-drug resistant cell; (3) expanding human CIAPIN1 gene cDNA by using the primers F and R and by adopting a PCR method; (4) constructing CIAPIN1 gene induction expression plasmids pET28-CIAPIN1 and pGEX-CIAPIN1; (5) constructing engineering bacteria B21-CIAPIN1 and DE3-CIAPIN1; (6) using the engineering bacteria B21-CIAPIN1 and DE3-CIAPIN1 to carry out prokaryotic expression and purification on CIAPIN1 protein; (7) using CIAPIN1 protein to immunize a 4-week-old Balb / C mouse so that mouse anti-human CIAPIN1 antibody is generated; and (8) using the serum of the immunized mouse to prepare the mouse anti-human CIAPIN1 monoclonal antibody. The invention can better identify multi-drug resistant gastric cancer and non-multi-drug resistant gastric cancer of clinical gastric cancer patients, and predict clinical prognosis of the gastric cancer patients.

The invention discloses a process for preparing phytase by efficiently fermenting recombinant pichiapastoris and a special batch fermentation culture medium thereof. The process comprises a thalli growth stage and an exogenous gene induction expression stage and relates to promoters such as an alcohol oxidase promoter, a methanol promoter and the like. The culture medium in a fed-batch fermentation stage adopts a low-salt formula which comprises the following components in percentage by weight: 3 to 6 percent of carbon source, 1.5 to 3 percent of nitrogen source, 0.8 to 1.0 percent of magnesium sulfate, 0.5 to 1.0 percent of potassium sulfate and 0.3 to 0.4 percent of monopotassium phosphate, wherein fermentation temperature is between 27 and 30 DEG C; and fermentation pH is between 4.5 and 5.5. Methanol induction in the exogenous gene induction expression stage is performed in five stages. The preparation process of the invention has the advantages of lower cost and higher phytase fermentation activity.

The invention relates to a genetic modification method for regulating and controlling animal endogenous gene expression. Tetracycline response elements are inserted into proper positions of endogenous genes of animals to be regulated and controlled according to fixed points through the gene recombination technology; and regulation and control genes are transferred into animal genomes by a transgene method, transgene animals are obtained, and the reversible expression regulation and control on the endogenous genes is realized under the effect of inductors of tetracycline or other analogues. A mouse endogenous gene induction expression regulation and control model built according to the method can be widely applied to the fields of gene function study, disease animal model building and the like.

Visual self-sucking method for inducing external gene into insect eggs is carried out by: under telescope, coating solution with external genes on insect eggs, pricking eggs to make external gene into eggs, making external genes combined with insect's gene to form transgenic insects. It makes technicians to do researches on transgenic insects with cheap instrument.

The invention relates to a construction method of an AD model rat hippocampus difference gene expression chart by combining Abeta and D-gal. The construction method comprises the following steps: firstly, treating a rat with the Abeta and the D-gal to establish an AD model rat; secondly, measuring memory damage of the AD model rat by using a Morris water maze so as to determine successful construction of the AD model rat; thirdly, obtaining hippocampus samples of an AD model group and a normal control group, and respectively obtaining gene chip data of all tested hippocampus gene expressions;fourthly, uploading all the gene chip data to gene chip data analysis software, obtaining differential expression genes and screening a gene spectrum of the differential expressions of the AD model rat; fifthly, carrying out functional annotation classification and a biological pathway analysis on the differently-expressed genes to reveal molecular basis associated with the AD. According to the construction method disclosed by the invention, the Abeta and the D-gal are used as a whole AD model to study the gene expression spectrum of the AD model rat hippocampus genes, so that other errors changing the expressions and induced by exogenous genes are effectively avoided.

The invention discloses a plant fertility associated protein and a coding gene and use thereof. The protein provided by the invention is named MYB21CDS and is derived from Arbidopsis thaliana. The protein may be a protein 1) and a protein 2), wherein the protein 1) is formed by an amino acid sequence represented by the sequence No.2 in a sequence table; and the protein 2) is formed by substituting and / or losing and / or increasing one or several amino acid residues in the amino acid sequence represented by the sequence No.2. Experiments conducted in the invention prove that the gene MYB21CDS provided by the invention leads to male sterility of plants after overexpression. In production, the gene can be expressed under induction to produce a sterile line which can serve as a female parent tobe crossed with a male parent to produce hybrid seeds.

A polytherapy of orally available compounds is disclosed that synergistically modulates and induces the expression of the cathelicidin gene (CAMP), which encodes the host defense peptide LL-37. By providing a number of different CAMP-inducing compounds together at the same time, stronger gene induction is achieved than with just one or two compounds, because the mechanism of induction broadens. Induction also may vary in different parts of the body depending on which compounds are used, and at what levels. We show for the first time that the polytherapy can induce cathelicidin expression in the brain, which may help to treat or prevent Alzheimer's Disease. Systemic cathelicidin gene induction may help treat numerous other conditions including Type 2 Diabetes / Metabolic Syndrome, or chronic bacterial, viral, or fungal infections associated with increased cancer risk or neurodegeneration. By increasing cellular autophagy and macroautophagy and supporting mitochondrial biogenesis and homeostasis, CAMP gene upregulation may reduce the effects of cellular aging and increase longevity.

The invention relates to a phage-related gene induced expression system, and particularly discloses a gene induced expression system which comprises host bacteria and a phage, wherein the host bacteria comprise a target gene, and the bacteriophage comprises a regulatory gene; the regulatory gene is a gene capable of expressing a regulatory factor after bacteriophage infects host bacteria, and the regulatory factor obtained by expression of the regulatory gene can specifically regulate a promoter of a target gene, or can specifically regulate expression of the target gene or modification after expression. According to the expression system, bacteriophage infection serves as an induction factor for the first time, expression of downstream target genes is started, and then expression of target genes or signal genes in specific host bacteria is achieved. The method provided by the invention not only realizes the expression of the target protein or the signal protein, but also realizes the expression of the target protein or the signal protein in the specific host bacteria, so that the host bacteria in the same culture system are distinguished based on whether the host bacteria are infected by the bacteriophage or not.

The invention discloses a peanut senescence gene for regulating programmed cell death, a coding sequence and application. RNA (ribonucleic acid) is extracted from root tip of peanut, and is cloned to a peanut senescence gene AhSAG, and the ORF (open read frame) of the peanut senescence gene is a cDNA (complementary deoxyribonucleic acid) sequence with coding sequence of 474bp and codes of 157aa; the AhSAG comes from a peanut root tip meristematic tissue of aluminum-induced peanut root tip generating programmed cell death, and is a senescence induction enhanced gene to induce, activate and promote progeria of plants. The peanut senescence disclosed by the invention can be expressed in tobacco, and can be used for regulating the adaptability of plant cells to environment changes and maintaining environmental stability and synthesis capacity in the cell.

The invention discloses an efficient genetic transformation method for agrobacterium-mediated sugarcane calluses, and belongs to the field of tissue culture. According to the invention, by enhancing the Agrobacterium tumefaciens Vir gene induction activation level and the bacterial liquid dip dyeing strength of sugarcane calluses, the occurrence frequency of transgenic events is greatly improved; an antioxidant is added into subsequent co-culture, screening and differential culture media to inhibit callus browning caused by agrobacterium-enhanced dip dyeing, so that the survival rate and the differentiation capacity of the dip-dyed calluses are obviously improved; a double-marker screening system with a green fluorescent protein marker EGFP gene and a herbicide glufosinate-ammonium resistance marker Bar gene at the same time is adopted, positive transgenic calluses are visually and nondestructively screened by using an EGFP marker, positive transgenic differentiated seedlings are screened by using Bar marker resistance, the accuracy of transgenic event screening is remarkably improved, and the screening efficiency is improved. And finally, the efficient genetic transformation of the agrobacterium tumefaciens mediated sugarcane callus is realized.

The invention relates to the field of animal medicine biological engineering, in particular to an infectious bursal disease virus DNA vaccine and a construction method thereof. The DNA vaccine comprises a three gene co-expression vector of IBDV VP2 gene, chicken Akirin2 gene and chicken GM-CSF gene, and independent and equal co-expression of the IBDV VP2 gene, the chicken Akirin2 gene and the chicken GM-CSF gene can be realized by one carrier. When the DNA vaccine is applied, the situations that the produced IBD antibody level and the provided protection rate are low when the VP2 gene performs immunization alone can be improved, chicken Akirin2 and chicken GM-CSF can form a synergistic effect on the biological function, and the immune response ability induced by the IBDV gene and resisting IBDV infection can be promoted jointly.

The invention belongs to the technical field of gene induction, and discloses a method for inducing a silent gene expression of marine actinomycetes to generate new substances. The method comprises the following steps: flushing a anthocidaris crassispina with sterile aged seawater, washing the anthocidaris crassispina with sterile water for 3 times, then placing the intestinal tissue thereof in the sterile water, diluting, then sucking 200 [mu]L and coating on a Gorodkowa medium plate containing 50 g / mL of potassium dichromate and 20 g / mL of kanamycin to obtain actinomycete strains; performinga small-scale fermentation on the strains, screening the ethyl acetate crude extract of fermentation liquids of 2 strains by adopting a plate filter paper method, and performing an activity screening; and detecting a new secondary metabolite generated by the induced strains. According to the method, a physical and chemical method is firstly adopted to induce the silent gene expression of marine actinomycetes to generate new substances, and a method for inducing and identifying the new substances generated by the marine actinomycetes is established through the combination of a spectral chemistry testing and an activity evaluation.

The present invention relates to fundamental discoveries for understanding the mechanism of non-gene targeting effects of steroids and its relation to gene targeting effects. It has been found that (i) non-gene targeting and gene targeting effects of steroids can be mediated through the same steroid receptors; (ii) both effects are ligand-induced; (iii) non-gene targeting effects occur is due to the ligand-inducing activity of the ligand-binding domain, which may be rapid and loose binding; (iv) gene targeting occurs due to the ligand of the DNA-binding domain of the steroid receptor induced activity, which is usually the result of a slower, stronger interaction; and (v) non-gene targeting of ligand interactions can be derived from gene targeting of ligand interactions separation so that selective reactions can be carried out. The present invention also discloses for the first time that certain steroids can induce non-gene targeting effects by binding to unrelated steroid receptors.

The invention provides a building method of a mice spontaneous glioma immunotherapy animal model. The method is characterized by building a rapid and spontaneous glioma model by using an SB vector local transgenic technology and inducing C57 / BL6 mice to generate spontaneous glioma by using an AID gene. SB is a system comprising two parts; the two parts comprise transposons DNA and transposase. AID is an enzyme capable of inducing DNA mutation in human genome under the physiological status and is capable of deaminizing cytosine on DNA and transforming cytosine into thymine. The spontaneous glioma model is capable of monitoring the growth of the tumor by using the bioluminescence technique; the speed of inducing the generation of the tumor of the spontaneous glioma model is equal to or superior to the virus-induced animal model; meanwhile, the mice spontaneous glioma immunotherapy animal model is free of disadvantages of long virus vector preparation time, high price and low tumorigenesis rate.

The invention discloses a method for breeding maize haploid inducer lines. By developing a method for identifying colored embryo haploids of the inducer lines, the haploid identification of the maize haploid inducer lines can be realized, thereby doubling, and The induction line DH line with high induction rate, high identification efficiency and excellent agronomic traits can be screened from four aspects of molecular marker-assisted selection: induction gene, induction rate, haploid identification efficiency and agronomic traits. The overall process takes 1 ‑2 years to obtain an excellent haploid induction line. The method created by the present invention, which can be used for rapid selection of haploid induction lines in maize, accelerates the breeding process of haploid induction lines, and provides a solution to the problems of long breeding cycle and heavy workload of haploid induction lines in maize. A feasible plan can greatly shorten the breeding period of haploid induction lines, reduce the testing workload of each generation, improve the efficiency of haploid breeding, and thus promote the development of maize haploid breeding technology, which is of great significance.

The present invention provides modified promoters from Candida troplicalis CYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

The invention relates to an accelerator and a preparation method thereof, belonging to the technical field of animal husbandry biological products; by weight percentage, it comprises: 82% corn steep liquor, 6% urea, 5% mannitol, 4% DL-methionine, and L-arginine 3%; a kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4 %; B. mixed and stirred in a dry powder mixer for 60 minutes to obtain the finished product; a kind of accelerator of 5-aminolevulinic acid comprises by weight percentage: lactose 12%, triammonium citrate 3.5%, L-asparagine 14%, hemoglobin 1.5%, L-serine 69%; the accelerator provided by the invention can increase the content of 5-aminolevulinic acid in the photosynthetic bacteria fermentation broth, and has the advantages of simple production process, easy operation, no transgene and gene induction It has the advantages of low energy consumption, easy access to raw materials, high yield, low cost, convenient use, and more obvious effects.

The invention provides an expression method of recombinant human insulin, a special expression vector, engineering bacteria and application. This expression method can induce the expression of recombinant human insulin gene in food-grade lactic acid bacteria and display recombinant human insulin on the surface of lactic acid bacteria. The engineering bacteria, expression vector and inducer used in this expression method all meet the requirements of food grade, avoiding the The presence of resistance genes on vectors and the potential harm caused by non-food-grade inducers, etc. The lactic acid bacteria engineering bacteria containing cell wall display insulin obtained by the present invention can stimulate NOD mice to produce recombinant human insulin-specific antibodies, significantly increase the level of cytokine IL-4 related to immune tolerance, and induce immune tolerance. The induced lactic acid bacteria engineered bacteria can be used as an oral vaccine for type 1 diabetes after being made into bacterial preparations, without the need for tedious post-processing such as purification, and has broad application prospects.

Visual self-sucking method for inducing external gene into insect eggs is carried out by: under telescope, coating solution with external genes on insect eggs, pricking eggs to make external gene into eggs, making external genes combined with insect's gene to form transgenic insects. It makes technicians to do researches on transgenic insects with cheap instrument.

The present invention relates to the protective effect and application of FSTL1 in the homeostasis regulation of tissues such as liver against fibrosis, in particular to the application of FSTL1 protein or FSTL1 regulating compound in the preparation of medicine for treating tissue fibrosis. The invention also relates to a conditional FSTL1 gene knockout non-human animal model closely related to the degree of tissue fibrosis and its preparation method, and its use in screening anti-tissue fibrosis drugs. Among them, by combining different doses of tamoxifen and different stages of animal growth, a non-human animal model of systemic or vascular endothelial cell FSTL1 gene-induced knockout is obtained to simulate different tissue fibrosis lesions.