10 results about "Gene induction" patented technology

The application provides a nano-catalytic material system capable of targeting tumors for gene editing, which is obtained by loading a CRISPR / Cas9 plasmid of a gene editing system capable of editing a core protein gene GPX4 related to cell ferroptosis into the pores of an iron single-atom nano-catalyst, and then modifying the cell membrane of a homologous tumor. The nano-catalytic material system provided by the application has excellent Fenton catalytic performance, can accurately edit target genes, induces tumor cells to undergo ferroptosis, and has the advantages of simple synthesis process, low cost, easy batch production, high enrichment degree at a lesion site, good treatment effect, and good clinical application prospect.

The present invention provides methods for controlling gene expression of gene 5 engineered organisms using inducer compounds. The invention also relates to genetically modifying microorganisms by using natural and / or non-natural inducer compounds, such as carbon compounds and / or nitrogen-containing compounds, such as sugars, saccharide analogs, nucleotides, and the like, to develop efficient gene regulatory mechanisms. The present invention also describes gene expression regulation and 10 modulation by the use of a unique natural compound guanidine, alone or in combination with other inducer compounds, thereby achieving efficient gene expression control.

This invention discloses a recombinant vector containing the LjARF25 gene from honeysuckle and its applications, belonging to the field of plant genetic engineering technology. The recombinant vector is based on the pCAMBIA series of binary plant expression vectors, containing an endogenous auxin-responsive promoter 2000 bp upstream of the LjARF25 gene as shown in SEQ ID NO:2 and the coding region sequence of the LjARF25 gene as shown in SEQ ID NO:1, constructing an LjARF25pro::LjARF25 inducible expression cassette. The LjARF25 gene responds to exogenous auxin stimulation, and its expression level shows significant time- and concentration-dependent changes after exogenous auxin treatment. This invention, by constructing an LjARF25 gene inducible expression vector suitable for honeysuckle, provides a technical foundation for honeysuckle gene function research, metabolic regulation of medicinal components, and molecular breeding.

The invention discloses engineering saccharomycetes for selectively recovering waste valuable metals as well as a construction method and application of the engineering saccharomycetes. The tested microorganism is saccharomyces cerevisiae and comprises a gene for coding an exogenous metal ion transport protein, an inducible promoter and a terminator, and the promoter is galactose GAL1; and the terminator is CYC1 (Cytochrome CYC1). According to the invention, metal ion transport protein, metabolic pathway regulation, biomineralization and other synthetic biological tools are utilized, so that the recovery selectivity of valuable metals is improved; a biological system is mild in culture, can adapt to a relatively complex wastewater environment, and is beneficial to large-scale industrial operation; and a green and low-cost preparation mode is provided for preparation of high-added-value products. In a coexistence environment, the engineering yeast selectively recovers valuable metal Cd and synthesizes CdSe quantum dots of 92.20 mg / g dry yeast, and the CdSe quantum dots are 1.29 times that of wild saccharomyces cerevisiae. The system has the advantages of high selectivity, low cost, environmental friendliness and the like, and is suitable for metal resource utilization of electronic wastes, industrial wastewater and other scenes.

The invention discloses a TALENs technology-assisted plant gene site-directed integration vector construction method, and belongs to the technical field of plant gene engineering. The method comprises the following specific steps: S1, cloning an environment response type promoter, and connecting the environment response type promoter to a vector skeleton; s2, designing a TALEN recognition module containing 19-25 RVD units, carrying out cloning and yeast homologous recombination double-platform assembly, enabling the GC content of a targeting sequence to be 40-60%, and adopting a dimer reverse cleavage mode; s3, integrating the homologous recombination template and the dual-fluorescence label; and S4, inserting a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) mediated off-target repair system and a TALEN-HAT fusion expression box. According to the invention, TALEN expression is driven by light / temperature sensitive and other environmental response type promoters, so that space-time precise regulation and control of gene editing are realized, continuous expression toxicity of constitutive promoters is avoided, and the promoter is suitable for stress-resistant gene induced expression.

Disclosed in the present invention is an optimized cell transplant. The optimized cell transplant is formed by performing gene induction and modification on a mesenchymal stem cell in the form of a small molecule and protein composition. The expression levels of CD200 gene, Galectin-9 gene and VISTA gene can be increased synchronously after cell culture. Vector virus infection and plasmid transfection are not required in the cell preparation process, so that high biological safety and great clinical application value of cells are achieved. The optimized cell transplant is suitable for the technical field of mesenchymal stem cells applied to cell transplantation therapy, and the therapeutic effect of the optimized cell transplant is more excellent than that of the non-modified mesenchymal stem cell.

The invention discloses a taxodium mexicana WRKY8 salt-tolerant gene and application thereof in regulating high-salt tolerance of plants and / or cultivating high-salt-tolerant plant varieties, also discloses a method for evaluating the salt tolerance of taxodium zhongshanha on the basis of WRKY8 gene induced expression amplitude, and belongs to the technical field of gene engineering. 1, and the WRKY8 gene codes an amino acid sequence as shown in SEQ ID NO. 2. By overexpressing the WRKY8 gene, the resistance of the plant to high-salt stress can be improved, the WRKY8 gene can be applied to plant genetic engineering breeding, and a new method is provided for creation or improvement of new high-salt-resistant germplasm of the plant.

Biomarkers for post-traumatic stress disorder (PTSD) and biomarkers for fibromyalgia (FM), which is associated with a high rate of PTSD, have been identified by narrowing down biomarker genes from PTSD-related gene database on the basis of biological events related to disease conditions. In addition, Chinese herbal medicines that are potential therapeutic agents for PTSD have been identified through the examination and comparison of gene induction by Chinese herbal medicines and antipsychotics in human keratinocytes. The present method can provide: biomarkers applicable as indicators for the diagnosis and the development of therapeutic agents for PTSD and FM; effective Chinese herbal medicines for PTSD and FM; and a screening method for therapeutic agents for PTSD and FM.

The present invention addresses the problem of providing a novel means for treating cancer by activating antitumor immunity in cancer cells. The present invention provides an antitumor immunity induction method comprising introducing a virus-derived gene or a gene whose expression is induced by the virus-derived gene into a cancer cell.

The application belongs to the field of microorganisms, and discloses an acidophilic thiobacillus and a gene scarless knockout method thereof, which comprises the following steps: recombining an I-SceI gene, an IPTG inducible promoter lacI-PtacO gene and a suicide plasmid pSDUDI to construct a pSDUDI-LacI-I-SceI gene knockout plasmid, and knocking out the I-SceI gene by IPTG induction and expression of I-SceI enzyme; the method improves the probability of the gene knockout plasmid part “separating” from the acidophilic thiobacillus chromosome, simplifies the operation genetic operation step by one-step method, is less time-consuming, and shortens the gene knockout time.