Silicatein-mediated synthesis of amorphous silicates and siloxanes and use thereof

a technology of silicatein and amorphous silicates, which is applied in the field of silicatein-mediated synthesis of amorphous silicates and siloxanes, can solve the problems of requiring drastic conditions in the chemical synthesis process of silicates, unable to meet the requirements of high-efficiency silicate synthesis, and can only be produced in a cost-intensive manner, so as to achieve fast and effective affinity chromatography purification of enzymes

Inactive Publication Date: 2007-01-30
NANOTECMARIN
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0040]By way of production of a specific polyclonal antibody and subsequent coupling to a solid

Problems solved by technology

Some of the above-mentioned silicon compounds can only be produced in a cost-intensive manner or are present only in small amounts as mineral resources, respectively, and can therefore only be isolated with considerable effort.
The process of the chemical synthesis of sili

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silicatein-mediated synthesis of amorphous silicates and siloxanes and use thereof
  • Silicatein-mediated synthesis of amorphous silicates and siloxanes and use thereof
  • Silicatein-mediated synthesis of amorphous silicates and siloxanes and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047]The silicatein protein derived from sponges contains characteristic sequence portions, several of which shall be mentioned: region around the serine-rich amino acid-cluster (in case of S. domuncula: amino acids 267–277); region around the cysteine, the first amino acid of the catalytic triade (in case of S. domuncula: amino acid 138), which is absent in silicatein and present in the related enzymes cathepsines; region around the transition between propeptide and the processed peptide (in case of S. domuncula: amino acids 112 / 113).

[0048]The gene for silicatein can be identified from cDNA-libraries by using the technique of the polymerase-chain reaction, e.g. in ZapExpress and in Escherichia coli XL1-Blue MRF′, using suitable degenerated primers (for example: reverse primer 5′-GAA / GCAG / CCGIGAIGAA / GTCA / GTAG / CAC-3′ together with the 5′-vector-specific primer [region around amino acids 267–277]—or the forward primer at the same protein-segment together with the 3′-vector-specific p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Silicatein is an enzyme of silicate-forming organisms used for the synthesis of their silicate scaffold. The present invention relates to the use of highly-expressed and highly active recombinant silicatein, silicatein isolated from natural sources after gene induction as well as silicatein-fusion proteins for the synthesis of amorphous silicon dioxide (silicic acids and silicates), siloxanes as well as modification of these compounds and their technical use.

Description

BACKGROUND OF THE INVENTION[0001]Silicon is the second-most element of the earth's crust (more than 80% of the earth's crust consist out of silicates) and is present in all forms of different compounds. Silicon compounds do not only represent most of the species of this class of minerals, but are also very important from an economical point of view. They are used in large scales and diverse forms. Glass, porcelain, emaillie, clay products, cement and water glass are technically important materials that consist out of silicates. The catalytic properties of some of the silicates are used synthetically. Their versatile uses are further expanded, if other elements, in particular aluminium, occupy some of the lattice positions that are otherwise occupied by silicon. Feldspars and zeolithes, for example, belong to these alumo silicates; the importance of the latter is based in particular in their molecular sieve and ions exchange properties. Al- and Ca-silicates have become important as f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P3/00A01N55/10C07H21/04C12N1/19C12N1/21C12N5/06C12P21/06C12N15/09C01B33/12C01B33/18C01B37/00C07K1/22C07K14/435C12N9/00C12N9/88C12R1/90H01L21/314
CPCC07K14/43504C12P3/00C12N9/88C12N9/00
Inventor MULLER, WERNER E. G.SCHRODER, HEINZ C.LORENZ, BERNDKRASKO, ANATOLI
Owner NANOTECMARIN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products