A kind of accelerator of 5-aminolevulinic acid and preparation method thereof

A technology of aminolevulinic acid and accelerator, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unstable transgenic strains, easy mutation of strains, unstable production, etc., and achieve no transgenics. And the effect of hidden dangers of gene mutation, easy availability of raw materials and low cost

Active Publication Date: 2017-12-08
山东环亿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two main methods of using microorganisms to produce 5-aminolevulinic acid: one is to introduce foreign genes through genetic engineering, and transform engineered strains to promote the production of related enzymes. The transgenic strains are unstable and prone to defects such as plasmid loss, and there are GMO Safety Controversy
The second is to carry out mutagenesis breeding of photosynthetic bacteria, select and breed mutant strains with high yield of 5-aminolevulinic acid and cultivate them on a specific medium, but the mutation of the strains in this method is non-directional, and the strains are prone to mutations, resulting in unstable production and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: corn steep liquor 82%, urea 6%, mannitol 5%, DL-methionine 4%, L-arginine 3%; Its preparation method is:

[0023] A. Add corn steep liquor, urea, mannitol, DL-methionine, and L-arginine into the dry powder mixer in proportion.

[0024] B. Mix and stir in a dry powder mixer for 60 minutes to obtain the finished product.

[0025] Wherein the consumption of each raw material by weight percentage is: corn steep liquor 82%, urea 6%, mannitol 5%, DL-methionine 4%, L-arginine 3%.

[0026] A set of comparative examples is included in Example 1.

Embodiment 2

[0047] A kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4%; Preparation The method steps are:

[0048] A. Add dipotassium hydrogen phosphate, L-cysteine ​​hydrochloride, L-alanine, and sodium chloride into the dry powder mixer in proportion.

[0049] B. Mix and stir in a dry powder mixer for 60 minutes to obtain the finished product.

[0050] Wherein the consumption of each raw material by weight percentage is: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4%.

Embodiment 3

[0060] A kind of accelerator of 5-aminolevulinic acid comprises: 12% of lactose, 3.5% of triammonium citrate, 14% of L-asparagine, 1.5% of hemoglobin, 69% of L-serine; the preparation method steps are:

[0061] A. Add lactose, triammonium citrate, L-asparagine, hematin, and L-serine into the dry powder mixer in proportion.

[0062] B. Mix and stir in a dry powder mixer for 60 minutes to obtain the finished product.

[0063] Wherein the consumption of each raw material by weight percentage is: 12% of lactose, 3.5% of triammonium citrate, 14% of L-asparagine, 1.5% of hemoglobin, 69% of L-serine.

[0064] Comparative Example 2

[0065] The specific operation steps of this comparative example are the same as those of comparative examples 1 and 2.

[0066] Table 1. Photosynthetic bacteria liquid rich in 5-aminolevulinic acid promoter

[0067] group

quantity

Average initial weight per head

Test 60 days average weight gain per head

die

Remark

te...

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Abstract

The invention relates to an accelerator and a preparation method thereof, belonging to the technical field of animal husbandry biological products; by weight percentage, it comprises: 82% corn steep liquor, 6% urea, 5% mannitol, 4% DL-methionine, and L-arginine 3%; a kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4 %; B. mixed and stirred in a dry powder mixer for 60 minutes to obtain the finished product; a kind of accelerator of 5-aminolevulinic acid comprises by weight percentage: lactose 12%, triammonium citrate 3.5%, L-asparagine 14%, hemoglobin 1.5%, L-serine 69%; the accelerator provided by the invention can increase the content of 5-aminolevulinic acid in the photosynthetic bacteria fermentation broth, and has the advantages of simple production process, easy operation, no transgene and gene induction It has the advantages of low energy consumption, easy access to raw materials, high yield, low cost, convenient use, and more obvious effects.

Description

technical field [0001] The invention relates to an accelerator and a preparation method thereof, belonging to the technical field of animal husbandry biological products. Background technique [0002] Studies have shown that 5-aminolevulinic acid and its derivatives have important application value in the fields of medicine, agriculture, animal husbandry, and daily chemicals. In agriculture, 5-aminolevulinic acid has been widely concerned as a pollution-free green herbicide. As a plant growth regulator, it has a good growth-promoting effect, participates in the regulation process of plant growth and development, regulates the synthesis and stability of chlorophyll, and improves Fruit quality; in medicine, 5-aminolevulinic acid, as a new generation of photodynamic drugs, is used in the diagnosis and treatment of cancer; in animal husbandry, it is used as a feed additive to promote growth and improve animal immunity and disease resistance; in daily cosmetics and Health food a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12R1/19
Inventor 张起凡曹崇仁朱友峰
Owner 山东环亿生物科技有限公司
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