Method for inducing silent gene expression of marine actinomycetes to generate new substances

A technology for marine actinomycetes and gene silencing, which is applied in biochemical equipment and methods, microbial determination/inspection, and measurement devices, etc., which can solve the problems that limit the discovery of lead compounds of original innovative drugs, the low yield of active ingredients, and the difficulty in discovering new activities. material ability

Inactive Publication Date: 2019-04-12
HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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  • Claims
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Problems solved by technology

[0007] (1) The yield of active ingredients produced by fermentation of wild actinomycete strains is often low, and sometimes even new compounds can be isolated, but the amount is too small to complete the work on their activity identification and other aspects
[...

Method used

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  • Method for inducing silent gene expression of marine actinomycetes to generate new substances
  • Method for inducing silent gene expression of marine actinomycetes to generate new substances
  • Method for inducing silent gene expression of marine actinomycetes to generate new substances

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Embodiment Construction

[0030] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0031] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0032] Such as figure 1 As shown, the method for inducing marine actinomycete silencing gene expression to produce new substances provided by the embodiments of the present invention is:

[0033] S101: Purple sea urchins were soaked in sterile old sea water for 3 times, each time for 10 minutes, then placed in 75% alcohol for 10 minutes, and then washed with sterile normal saline for 3 times, and the surface moisture was blotted with sterile filter paper before cleaning. Carefully di...

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Abstract

The invention belongs to the technical field of gene induction, and discloses a method for inducing a silent gene expression of marine actinomycetes to generate new substances. The method comprises the following steps: flushing a anthocidaris crassispina with sterile aged seawater, washing the anthocidaris crassispina with sterile water for 3 times, then placing the intestinal tissue thereof in the sterile water, diluting, then sucking 200 [mu]L and coating on a Gorodkowa medium plate containing 50 g/mL of potassium dichromate and 20 g/mL of kanamycin to obtain actinomycete strains; performinga small-scale fermentation on the strains, screening the ethyl acetate crude extract of fermentation liquids of 2 strains by adopting a plate filter paper method, and performing an activity screening; and detecting a new secondary metabolite generated by the induced strains. According to the method, a physical and chemical method is firstly adopted to induce the silent gene expression of marine actinomycetes to generate new substances, and a method for inducing and identifying the new substances generated by the marine actinomycetes is established through the combination of a spectral chemistry testing and an activity evaluation.

Description

technical field [0001] The invention belongs to the technical field of gene induction, and in particular relates to a method for inducing marine actinomycetes to express silent genes and produce new substances. Background technique [0002] At present, the existing technology commonly used in the industry is as follows: more than 50% of the active substances produced by microorganisms come from actinomycetes. Due to the outstanding ability of actinomycetes in producing new active metabolites, scientists have We have been working on the isolation of novel actinomycetes from terrestrial microbial sources. However, the number of novel compounds isolated from terrestrial actinomycetes has continued to decrease in recent years, so there is a need to find novel actinomycetes from unexplored actinomycete habitats as a source for the development of novel active secondary metabolites . Due to the particularity of the marine environment, marine actinomycetes differ from terrestrial ...

Claims

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Application Information

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IPC IPC(8): G01N30/89G01N30/02G01N30/06G01N30/14G01N30/72G01N30/74G01N30/78G01N30/62G01N30/90G01N30/88C12Q1/18
CPCC12Q1/18G01N30/02G01N30/06G01N30/14G01N30/62G01N30/72G01N30/74G01N30/78G01N30/88G01N30/89G01N30/90G01N2030/062G01N2030/77G01N2030/8813
Inventor 王蓉谭围陈傅晓冯全英樊佳伟王荣霞柯宏基
Owner HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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