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Electrochemical luminescence aptamer sensor for specifically detecting kanamycin as well as preparation method and application of electrochemical luminescence aptamer sensor

An aptamer sensor, kanamycin technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc., can solve the complex and time-consuming pretreatment process and difficult on-site detection , The problem of high degree of professionalism in instrument operation, to achieve the effect of high sensitivity, prevention of oxidation, and high specific surface area

Active Publication Date: 2021-03-16
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most instrumental analysis methods have limitations in terms of the cost of expensive equipment, high professionalism in instrument operation, complex and time-consuming pretreatment process, and it is difficult to promote to the market for on-site testing

Method used

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  • Electrochemical luminescence aptamer sensor for specifically detecting kanamycin as well as preparation method and application of electrochemical luminescence aptamer sensor
  • Electrochemical luminescence aptamer sensor for specifically detecting kanamycin as well as preparation method and application of electrochemical luminescence aptamer sensor
  • Electrochemical luminescence aptamer sensor for specifically detecting kanamycin as well as preparation method and application of electrochemical luminescence aptamer sensor

Examples

Experimental program
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Embodiment 1

[0033] (1) Preparation of silver nanoparticles (AgNPs) loaded on highly luminescent polydopamine nanospheres (HLPNs) composites:

[0034] Dissolve 0.0378g of dopamine hydrochloride in 12mL of phosphate buffer solution (pH 10), and add 8mL of H 2 o 2 , reacted at room temperature in the dark for 6 hours. After the reaction, the resulting solution was transferred to a dialysis membrane and dialyzed in ultrapure water for 4 hours. Finally, the solution in the bag was taken out and freeze-dried to obtain a light yellow solid, which was dried at 60°C , to obtain yellow powder, namely HLPNs.

[0035] Disperse 4mg of HLPNs in 10mL of ultrapure water, weigh 556mg of sodium citrate and dissolve in 40mL of freshly prepared AgNO 3 (0.025M), the two were mixed, placed in nitrogen and stirred at room temperature for 3 h, centrifuged, washed, and dried to obtain a reddish-brown solid, namely HLPNs@Ag.

[0036] (2) Preparation of black phosphorus quantum dots:

[0037] Disperse 5 mg of b...

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Abstract

The invention provides a method for specifically detecting kanamycin based on an electrochemical luminescence aptamer sensor, and particularly belongs to the field of electrochemical luminescence detection. The method comprises the steps of (1) loading silver nanoparticles on a composite material (HLPNs@Ag) of high-luminescence polydopamine nanospheres, and preparing black phosphorus quantum dots;(2) preparing an electrochemical luminescence aptamer sensor; and (3) jointly modifying the surface of a glassy carbon electrode by utilizing electrostatic adsorption combination between the HLPNs@Agand the black phosphorus quantum dots to improve the sensitivity and stability of electrochemical luminescence, and then loading the aptamer to obtain the electrochemical luminescence aptamer sensor.The sensor can specifically recognize kanamycin, the detection range is 1.0*10<-12>mol / L-1.0*10<-7>mol / L, and the lowest detection limit is 1.7*10<-13>mol / L. According to the invention, the kanamycindetection sensitivity is high, the specificity is strong, and the operation is simple.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence detection, and relates to a method for specifically detecting kanamycin based on an electrochemiluminescence aptamer sensor. In particular, it involves the loading of aptamer molecules on the surface of glassy carbon electrodes modified by high-luminescence polydopamine nanosphere composites (HLPNs@Ag / BP) doped with black phosphorus quantum dots and silver nanoparticles, that is, COOH-apt / HLPNs@ The Ag / BP / GCE electrode is used as a sensing element, and it is an electrochemiluminescence analysis method for quantitatively detecting kanamycin in river water. Background technique [0002] With the rapid development of science and technology and the increasing material demand of people, a series of environmental problems have erupted. The vast majority of antibiotics are water-soluble. After antibiotics are ingested, most of them are excreted into the environment as metabolites through feces an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/76G01N27/48
CPCG01N27/3278G01N27/48G01N21/76
Inventor 陈智栋温静单学凌蒋鼎
Owner CHANGZHOU UNIV
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