Building method of mice spontaneous glioma immunotherapy animal model

Abstract

The invention provides a building method of a mice spontaneous glioma immunotherapy animal model. The method is characterized by building a rapid and spontaneous glioma model by using an SB vector local transgenic technology and inducing C57 / BL6 mice to generate spontaneous glioma by using an AID gene. SB is a system comprising two parts; the two parts comprise transposons DNA and transposase. AID is an enzyme capable of inducing DNA mutation in human genome under the physiological status and is capable of deaminizing cytosine on DNA and transforming cytosine into thymine. The spontaneous glioma model is capable of monitoring the growth of the tumor by using the bioluminescence technique; the speed of inducing the generation of the tumor of the spontaneous glioma model is equal to or superior to the virus-induced animal model; meanwhile, the mice spontaneous glioma immunotherapy animal model is free of disadvantages of long virus vector preparation time, high price and low tumorigenesis rate.

Description

technical field [0001] The invention belongs to a method for establishing a spontaneous glioma immunotherapy animal model. Background technique [0002] Glioma is the most common primary malignant tumor in the brain, accounting for about half of the primary brain tumors in adults. Despite the continuous progress in surgical treatment, radiotherapy and chemotherapy, the prognosis of patients with glioma has not been significantly improved. The average survival time of patients with glioma is only about one year. [0003] In recent years, a lot of progress has been made in the immunotherapy of glioma in the world. Most of the glioma animal models used in these studies are glioma cell line transplanted tumor models in the brain. However, experiments have confirmed that glioma cells After a long period of culture, the expression of various proteins has changed, and it no longer has the original tumor biological characteristics. In addition, these animal models cannot reflect t...

AI Technical Summary

Problems solved by technology

[0003] In recent years, a lot of progress has been made in the immunotherapy of glioma in the world. Most of the glioma animal models used in these studies are glioma cell line transplanted tumor models in the brain. However, experiments have confirmed that glioma cells After a long period of culture, the expression of various proteins has changed, and it no longer has the original tumor biological characteristics

In addition, these animal models cannot reflect the heterozygosity characteristics of glioma, and cannot reflect the interaction between glioma and the immune system