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Building method of mice spontaneous glioma immunotherapy animal model

An immunotherapy and animal model technology, applied in biochemical equipment and methods, microorganisms, and the use of vectors to introduce foreign genetic material, etc., can solve the problem of no longer having tumor biological traits, unable to respond to the interaction between glioma and the immune system, Can not reflect the heterozygosity characteristics of glioma and other issues

Inactive Publication Date: 2016-10-26
吴安华
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Problems solved by technology

[0003] In recent years, a lot of progress has been made in the immunotherapy of glioma in the world. Most of the glioma animal models used in these studies are glioma cell line transplanted tumor models in the brain. However, experiments have confirmed that glioma cells After a long period of culture, the expression of various proteins has changed, and it no longer has the original tumor biological characteristics
In addition, these animal models cannot reflect the heterozygosity characteristics of glioma, and cannot reflect the interaction between glioma and the immune system

Method used

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Embodiment Construction

[0009] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail in conjunction with specific embodiments.

[0010] Materials: pT2 / C-luc / PGK-SB13 plasmid, pT / CAGGS-NRASV12 plasmid: a kind gift from the John Ohlfest laboratory.

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Abstract

The invention provides a building method of a mice spontaneous glioma immunotherapy animal model. The method is characterized by building a rapid and spontaneous glioma model by using an SB vector local transgenic technology and inducing C57 / BL6 mice to generate spontaneous glioma by using an AID gene. SB is a system comprising two parts; the two parts comprise transposons DNA and transposase. AID is an enzyme capable of inducing DNA mutation in human genome under the physiological status and is capable of deaminizing cytosine on DNA and transforming cytosine into thymine. The spontaneous glioma model is capable of monitoring the growth of the tumor by using the bioluminescence technique; the speed of inducing the generation of the tumor of the spontaneous glioma model is equal to or superior to the virus-induced animal model; meanwhile, the mice spontaneous glioma immunotherapy animal model is free of disadvantages of long virus vector preparation time, high price and low tumorigenesis rate.

Description

technical field [0001] The invention belongs to a method for establishing a spontaneous glioma immunotherapy animal model. Background technique [0002] Glioma is the most common primary malignant tumor in the brain, accounting for about half of the primary brain tumors in adults. Despite the continuous progress in surgical treatment, radiotherapy and chemotherapy, the prognosis of patients with glioma has not been significantly improved. The average survival time of patients with glioma is only about one year. [0003] In recent years, a lot of progress has been made in the immunotherapy of glioma in the world. Most of the glioma animal models used in these studies are glioma cell line transplanted tumor models in the brain. However, experiments have confirmed that glioma cells After a long period of culture, the expression of various proteins has changed, and it no longer has the original tumor biological characteristics. In addition, these animal models cannot reflect t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85A01K67/027
CPCC12N15/8509A01K67/0275A01K2217/05A01K2227/105A01K2267/0331C12N2800/107C12N2800/90
Inventor 吴安华
Owner 吴安华
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