A kind of method and its application of fast breeding maize haploid induction line

A haploid induction system and haploid technology, applied in the biological field, can solve the problems of long field test work, limited breeding cycle, and inability to identify haploids, so as to improve the efficiency of haploid breeding, promote development, The effect of reducing test workload

Active Publication Date: 2022-06-21
CHINA AGRI UNIV
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Problems solved by technology

Heavy field tests and long breeding cycles greatly limit the breeding process of maize haploid induction lines
The haploid breeding technology can greatly shorten the breeding cycle of pure lines and reduce human and economic costs. However, the haploid induction lines of maize currently used in practice all carry the R1-nj marker, and the embryos and endosperms of the grains are purple. Conventional methods cannot identify its haploid

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  • A kind of method and its application of fast breeding maize haploid induction line
  • A kind of method and its application of fast breeding maize haploid induction line
  • A kind of method and its application of fast breeding maize haploid induction line

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Effect test

Embodiment 1

[0047] Example 1 Breeding maize haploid induction line

[0048] 1. Construction of the basic material of the inducible line

[0049] In the winter of 2019, in Hainan, the maize haploid inducible lines CAU3 (induction rate of about 10%) and CAU6 (induction rate of about 12%) were planted, with CAU3 as the female parent and CAU6 as the male parent to assemble the hybrid combination CAU3 / CAU6, Harvest F 1 Seeds (F of CAU3 / CAU6 1 generation). the F 1 Seeds are the basic material.

[0050] 2. Hybrid induction to obtain candidate material groups containing haploids

[0051] Planting CAU3 / CAU6 F in Beijing in the spring of 2020 1 The generation was used as the female parent (induced material), and the inductive line CAU5 (induction rate was about 10%) was used as the male parent for induction. 15 days after pollination, the hybrid embryos were stripped and placed on MS solid medium for culture. The ploidy candidate material group (haploid immature embryos to be identified), a...

Embodiment 2

[0096] Example 2 Identification of inducible haploids based on oil content

[0097] 1. Induction of inducible haploids and determination of oil content

[0098] In the spring of 2018 in Beijing, the maize haploid inducible lines CAU3 (induction rate of about 10%) and CAU6 (induction rate of about 12%) were planted, with CAU3 as the female parent and CAU6 as the male parent to create a hybrid combination CAU3 / CAU6, Harvest F 1 Seeds have an oil content of about 3%.

[0099] In the winter of 2019, Hainan, planting CAU3 / CAU6 F 1 As the female parent (induced material), the high-oil inducible line CHOI4-1 (induction rate of about 12% and oil content of about 8%) was used as the male parent for hybridization. A sorter (model: nuclear magnetic resonance oil moisture content analyzer PQ001-20-025V, Suzhou Niumai Analytical Instrument Co., Ltd.) was used to measure the oil content of the grains. For the specific oil content distribution, see image 3 and Table 5.

[0100] Table 5...

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Abstract

The invention discloses a method for breeding maize haploid inducer lines. By developing a method for identifying colored embryo haploids of the inducer lines, the haploid identification of the maize haploid inducer lines can be realized, thereby doubling, and The induction line DH line with high induction rate, high identification efficiency and excellent agronomic traits can be screened from four aspects of molecular marker-assisted selection: induction gene, induction rate, haploid identification efficiency and agronomic traits. The overall process takes 1 ‑2 years to obtain an excellent haploid induction line. The method created by the present invention, which can be used for rapid selection of haploid induction lines in maize, accelerates the breeding process of haploid induction lines, and provides a solution to the problems of long breeding cycle and heavy workload of haploid induction lines in maize. A feasible plan can greatly shorten the breeding period of haploid induction lines, reduce the testing workload of each generation, improve the efficiency of haploid breeding, and thus promote the development of maize haploid breeding technology, which is of great significance.

Description

technical field [0001] The present invention relates to a method for rapid breeding of maize haploid inductive lines in the field of biotechnology and its application. Background technique [0002] As one of the three major modern biotechnologies, maize haploid breeding technology is widely used in breeding and scientific research. The haploid inducible line is the first condition to realize the haploid breeding technology, so a lot of researches have been carried out on the haploid inducible line in recent years. Existing inducible lines are all derived from Stock 6, with an induction rate of 3.2% (Coe, 1959). With manual test selection and molecular marker-assisted selection, a large number of maize haploid high-frequency inducible lines have been cultivated, mainly including CAU series, CHOI series, UH series, RWS / K series, TAIL series and PHI series, etc. (Ren et al, 2017), the haploid induction rate increased by about 10%. The continuous improvement of the haploid ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 陈绍江陈琛刘晨旭肖子健钟裕焦炎炎陈明李金龙
Owner CHINA AGRI UNIV
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