7 results about "Cellular Autophagy" patented technology

This invention provides a highly water-soluble composition for promoting autophagy and its application. The composition comprises 5-hydroxyflavone, acetylhydroxyproline, and a suitable solvent. This composition is a unique vesicular composition that enables efficient delivery and synergistic effects of the two active ingredients. It promotes autophagy, has improved solubility, stability, and antioxidant properties, and can prevent and repair skin photodamage at its source. This invention also discloses a method for preparing this composition and its applications.

The application discloses an immune cell autophagy marker detection kit and belongs to the technical field of biomedical detection, comprising two kinds of fluorescent coding microsphere mixtures coupled with anti-LC3B-II and p62 / SQSTM1 capture antibodies respectively, a biotinylated detection antibody mixture, a streptavidin-phycoerythrin conjugate, six-point calibrators (S0-S5), a quality control product, a cell lysis buffer containing non-ionic and zwitterionic surfactants and 4 mol / L urea, and data analysis software based on LC3B-II / p62 natural logarithm ratio output autophagy activity comprehensive score.

This invention belongs to the field of zoonotic disease prevention and control and molecular immunology technology, specifically relating to the application of a biological product that silences or knocks out the ID2 gene in regulating host resistance to Brucella infection. This invention constructs an ID2 gene knockout macrophage model using CRISPR / Cas9 gene editing technology and establishes a Brucella infection system. Experiments show that ID2 gene knockout significantly enhances the host's ability to clear Brucella. RNA-seq analysis shows that ID2 participates in regulating the host's transcriptional response network to Brucella, with its differentially expressed genes significantly enriched in lipid metabolism and autophagy. Mechanistic results indicate that the ID2 gene participates in the regulation of Brucella-mediated lipophage. It can be seen that the ID2 gene affects the intracellular replication level and lipophage process of Brucella. This invention provides new targets and theoretical basis for drug development against Brucella infection, immune regulation, and disease-resistant breeding.

This application discloses the use of tetrandrine in the preparation of products for treating autophagy-related diseases. Through research, this application found that tetrandrine can significantly induce autophagy and promote TFEB nuclear translocation, making it suitable as an autophagy inducer and TFEB activator. Furthermore, it is naturally derived, has low toxicity, and high activity, providing a safe and effective new strategy for treating autophagy-related diseases, especially Gaucher disease, a lysosomal storage disorder.

The invention relates to means and methods for the treatment of diseases involving autophagy by cells, which process according to the invention is involved in mechanisms of tissue repair, vascular permeability and immune responses. The invention provides methods and means to target the elastin receptor complex specifically and to provide molecules and compositions comprising a specific targeting agent as well as amino acid compositions that are involved in the pathway of autophagy and the diseases related thereto. The disclosure also relates to peptide-drug development, in particular to (the improvement of) autophagy inhibiting amino acid containing peptides, more in particular glutamine-containing peptides and / or glutamine and other autophagy modulating amino acid containing compositions useful in the treatment of vascular and inflammatory conditions. The invention further relates to the improvement of glutamine peptides useful in the treatment of diabetic, vascular and / or inflammatory conditions. The invention provides a Q- ER peptide, comprising a synthetic peptide or functional analogue thereof provided with at least one PG-domain amino acid motif xGxxPG or functional equivalent thereof, said PG- domain motif allowing targeting of said peptide to the elastin receptor complex (ER), wherein at least one amino acid at position x is selected from the group of amino acids alanine (in one letter code: A), glutamine (Q), glycine (G), valine (V), leucine (L), isoleucine (I), proline (P) and arginine, said peptide provided with at least one glutamine (Q).

The present application relates to a kind of activation of cell autophagy Epimedium extract and its preparation method and application, the present application is prepared using specific fermentation bacteria and composite enzyme Epimedium extract, improve the content of Epimedium in Epimedium extract Epimedium secondary glycoside I, the Epimedium extract can be up-regulated lysosome mannose glycosidase (LManV) expression, significantly enhance cell autophagy flow, promote autophagosome and lysosome fusion, efficiently reverse the p62 / Ref (2) P accumulation caused by autophagy function decline, maintain intracellular environment steady, delay cell aging speed, provide support for organism anti-aging from cell level, help to improve the physical condition related to aging, maintain organism activity, and safe and nontoxic to human body.