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Application of tanshinone I in preparation of tumor cell autophagy inducer drugs

A technology of tumor cells and tanshinone, applied in the new application field of tanshinone I, can solve the problems of decreased patient tolerance, cytotoxic side effects, unsatisfactory treatment effect, etc., and achieve the effect of inhibiting damage and autophagy, and the treatment effect is remarkable

Active Publication Date: 2020-04-14
YANGZHOU UNIV
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Problems solved by technology

Although chemotherapy and radiotherapy can delay tumor progression and prolong the survival of patients, it will also bring serious toxic and side effects of chemotherapy and radiotherapy, resulting in decreased tolerance of patients and affecting the therapeutic effect. Radiotherapy often produces toxicity to surrounding normal tissues, causing multiple Intractable complications, such as decreased vaginal elasticity, radiation cystitis, etc., seriously affect the quality of life of patients
Chemotherapy not only has a killing effect on cancer cells, but also has toxic side effects on normal body cells, and it will produce drug resistance, and the therapeutic effect is not satisfactory

Method used

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  • Application of tanshinone I in preparation of tumor cell autophagy inducer drugs
  • Application of tanshinone I in preparation of tumor cell autophagy inducer drugs
  • Application of tanshinone I in preparation of tumor cell autophagy inducer drugs

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Experimental program
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Embodiment

[0025] 1. Experimental techniques and methods

[0026] (1) Cell proliferation experiment

[0027] Hela and Siha cells were inoculated in 96-well plates at 1x10^5 cells / ml, and after 2 hours, the cells adhered to the wall, and different concentrations of Tanshinone I (0, 12.5, 25, 50 μM) and DMSO solvent control group were added, 37 ° C, 5% CO 2 After incubating in the incubator for 24h and 48h, add 10 μL of CCK8 solution to each well, shake well, place in the incubator for 1h, and use a microplate reader to detect the optical density value (OD value) of each well at the wavelength of 450nm.

[0028] (2) Detection of cell ultrastructure

[0029] Divide cells by 5*10 5 Cells / ml were inoculated on 10cm culture dishes, 8 dishes in each of the control group and the experimental group. After 24 hours of culture, except the control group, 50μM Tanshinone I treated the cells. After the drug effect reached 24 hours, transfer 8 dishes of the cell suspension into a 15mL centrifuge tub...

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Abstract

The invention discloses application of tanshinone I in preparation of tumor cell autophagy inducer drugs. Compared with the prior art, the invention for the first time finds that tanshinone I can induce autophagy of tumor cells and has a remarkable treatment effect on cervical cancer, breast cancer or liver cancer, especially cervical cancer. Research proves that tanshinone I can inhibit cancer cell growth, inhibit cancer cell mitochondrial injury and autophagy, and induce the expression of tumor cell autophagy molecules and cutting of autophagy molecule LC3.

Description

technical field [0001] The invention belongs to the technical field of new uses of tanshinone I, and in particular relates to the application of tanshinone I in the preparation of tumor cell autophagy inducer drugs. Background technique [0002] Cervical cancer is a common malignant tumor in women. It has a high degree of malignancy, high incidence and postoperative recurrence rate, and seriously endangers the physical and mental health of women. The treatment of cervical cancer includes surgery, radiotherapy and chemotherapy, and traditional Chinese medicine treatment. Surgical treatment is currently the most effective and feasible diagnosis and treatment option, but surgery is mainly suitable for patients whose disease is below stage IIB, and has limitations. For patients with advanced cervical cancer, the main treatment options are radiotherapy and chemotherapy. Although chemotherapy and radiotherapy can delay tumor progression and prolong the survival of patients, it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61P35/00
CPCA61K31/58A61P35/00
Inventor 崔树娜陈婷婷王梦梦
Owner YANGZHOU UNIV
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