Application of tanshinone i in the preparation of tumor cell autophagy inducer medicine
A tumor cell, tanshinone technology, applied in the new application field of tanshinone I, can solve the problems affecting the quality of life of patients, causing drug resistance, cytotoxic side effects, etc. significant effect
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[0025] 1. Experimental techniques and methods
[0026] (1) Cell proliferation experiment
[0027] Hela and Siha cells were inoculated in 96-well plates at 1x10^5 cells / ml, and after 2 hours, the cells adhered to the wall, and different concentrations of Tanshinone I (0, 12.5, 25, 50 μM) and DMSO solvent control group were added, 37 ° C, 5% CO 2 After incubating in the incubator for 24h and 48h, add 10 μL of CCK8 solution to each well, shake well, place in the incubator for 1h, and use a microplate reader to detect the optical density value (OD value) of each well at the wavelength of 450nm.
[0028] (2) Detection of cell ultrastructure
[0029] Divide cells by 5*10 5 Cells / ml were inoculated on 10cm culture dishes, 8 dishes in each of the control group and the experimental group. After 24 hours of culture, except the control group, 50μM Tanshinone I treated the cells. After the drug effect reached 24 hours, transfer 8 dishes of the cell suspension into a 15mL centrifuge tub...
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