The invention relates to a rapid positioning method of cotton
single locus quality
gene in
chromosome, and belongs to the technical field of
biology. The method includes establishing a near-isogenic mixed
pool by using land-sea F2 positioning group, screening the
gene to obtain a primer with polymorphism, detecting the
DNA of 9 single strains, P-1, P-2 and F1 in a recessive
gene mixed
pool, recording the banding patterns of the
DNA, calculating the individual number among the 9 single strains in the recessive mixed
pool which has the same banding pattern with the recessive parent P-1, calculating the
coincidence rate, selecting the polymorphic primer having the banding pattern
coincidence rate over 60% with P-1 and having dominant or codominant F1 banding pattern, detecting the F2 segregation
population by using the small amount of the primer, calculating the banding pattern, and analyzing by
software to obtain the SSR primer concatenated with the quality gene. Displayed by the embodiment, the method makes the range of screening target concatenated primer by using F2 positioning group reduce over 90%, can lock the SSR primer concatenated with the target quality gene rapidly, can reduce the
workload of the gene positioning effectively, and can improve the positioning efficiency of the quality gene in the
chromosome greatly.