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Nano-medicine, preparation method thereof and application of nano-medicine

A technology of nano-drugs and chemotherapeutic drugs, applied in the fields of nano-drugs, nano-technology, nano-technology, etc., can solve the problems of cytotoxicity, poor biocompatibility of gold nanorods, inability to carry drugs and photothermal agents, etc. Significant tumor and curative effect

Active Publication Date: 2020-02-28
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gold nanorods coated with the material have poor biocompatibility and cytotoxicity, which may cause inflammatory reactions and cannot solve the problem of cell autophagy caused by the photothermal effect.
[0005] CN107309437A discloses a gold nanostar / calcium phosphate nanoparticle, the photothermal conversion efficiency of the gold nanostar / calcium phosphate nanoparticle is high, and the thermal imaging of the tumor site can be realized while the drug is delivered, and the gold nanostar / calcium phosphate nanoparticle Particles are mainly used for thermal imaging of tumor sites, and cannot be loaded with drugs and photothermal agents for tumor treatment

Method used

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  • Nano-medicine, preparation method thereof and application of nano-medicine
  • Nano-medicine, preparation method thereof and application of nano-medicine

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Experimental program
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Embodiment 1

[0062] The preparation of embodiment 1 nano drug

[0063] (1) Dissolve 0.1 mL of docetaxel with a concentration of 1 μg / mL in methanol, add 1 mL of sodium polyphosphate solution with a concentration of 20 mg / mL to obtain the first mixed solution; Calcium chloride solution was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after 30 minutes of magnetic stirring at room temperature, and the precipitate was ultrasonically reconstituted to obtain the first dispersion, which contained docetaxel-encapsulated Calcium phosphate matrix;

[0064] (2) Add 10 mL of dopamine with a concentration of 1 mg / mL, adjust the pH to 8.5, centrifuge after 15 hours of magnetic stirring at room temperature, redissolve the precipitate and centrifuge, wash 3 times and redissolve the precipitate ultrasonically to obtain the second dispersion liquid. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion;

[0065] (3) Add 1 m...

Embodiment 2

[0067] The preparation of embodiment 2 nano medicine

[0068] (1) Take 0.1 mL of paclitaxel with a concentration of 1.5 μg / mL and dissolve it in acetonitrile, add 1 mL of sodium polyphosphate solution with a concentration of 30 mg / mL to obtain the first mixed solution; The solution was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after magnetic stirring at room temperature for 60 minutes, and the precipitate was ultrasonically redissolved to obtain the first dispersion, which contained paclitaxel (DTX) encapsulated calcium phosphate matrix;

[0069] (2) Add 10 mL of dopamine with a concentration of 2 mg / mL, adjust the pH to 9, centrifuge after 20 hours of magnetic stirring at room temperature, redissolve the precipitate and centrifuge, wash 4 times and redissolve the precipitate ultrasonically to obtain the second dispersion liquid. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion;

[007...

Embodiment 3

[0072] The preparation of embodiment 3 nano medicine

[0073] (1) Dissolve 0.1 mL of camptothecin with a concentration of 0.5 μg / mL in dimethyl sulfoxide, and add 1 mL of sodium polyphosphate solution with a concentration of 10 mg / mL to obtain the first mixed solution; Calcium chloride solution per mL was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after magnetic stirring at room temperature for 10 minutes, and the precipitate was ultrasonically redissolved to obtain the first dispersion, which contained Calcium phosphate matrix of resin;

[0074] (2) Add 10 mL of dopamine with a concentration of 0.5 mg / mL, adjust the pH to 7, centrifuge after magnetic stirring at room temperature for 10 h, redissolve the precipitate and centrifuge, wash twice, and redissolve the precipitate ultrasonically to obtain the second dispersion. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion liquid;

[0075] ...

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Abstract

The invention provides a nano-medicine, a preparation method thereof and an application of the nano-medicine. The nano-medicine comprises a calcium phosphate substrate, a chemotherapy medicine, a photo-thermal agent and an autophagy inhibitor, the chemotherapy medicine is sealed in the calcium phosphate substrate, the surface of the calcium phosphate substrate is modified by the photo-thermal agent, and the autophagy inhibitor is loaded into a pore of the photo-thermal agent. According to the nano-medicine, chemotherapy and thermotherapy are combined, calcium phosphate serves as a rigid substrate, the chemotherapy medicine and the photo-thermal agent are sealed in the rigid substrate of the calcium phosphate, the autophagy inhibitor of a cell is loaded on the rigid substrate, so that chemotherapy and thermotherapy effects can be improved by inhibiting autophagy, and tumor treatment effects are improved.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine carrier materials, relates to a nano-medicine and its preparation method and application, in particular to a nano-medicine capable of chemotherapy and hyperthermia sensitization by inhibiting autophagy, and its preparation method and application. Background technique [0002] The limitation of cancer treatment lies in the single treatment method and the lack of effective chemotherapeutic drug targeting strategies, making it difficult to achieve significant curative effect. Chemotherapy is one of the most commonly used clinical treatment methods for breast cancer, but the currently used chemotherapeutic drugs lack effective carriers, have poor specificity, and are often accompanied by severe side effects. Photothermal therapy (PTT) is a cancer treatment method with broad application prospects. With the assistance of photothermal agents, energy induces cancer cell death through the phototherma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/18A61K31/337A61K41/00A61P35/00B82Y5/00
CPCA61K9/5115A61K9/5123A61K31/337A61K41/0052A61P35/00B82Y5/00A61K2300/00
Inventor 吴雁王绚
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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