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Application of specnuezhenide as autophagy inducer

A kind of special privet glycoside and inducer technology, applied in the field of medicine

Inactive Publication Date: 2019-04-16
高亮亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing autophagy inducers have fewer types to choose from, most of them are synthetic compound molecules, and new autophagy inducers need to be developed

Method used

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  • Application of specnuezhenide as autophagy inducer
  • Application of specnuezhenide as autophagy inducer
  • Application of specnuezhenide as autophagy inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 The detection of teruzhenin in inducing autophagy of human lung cancer epithelial cells (A549)

[0042] Experimental materials: teruci, methicillin-resistant Staphylococcus aureus (MRSA), human lung cancer epithelial cells, that is, A549 cells (cultivated in DMEM medium containing 10% fetal bovine serum), DMEM was purchased from Hyclone Corporation of the United States , product number SH30022.01; fetal bovine serum was purchased from Shanghai Jitai Yikesai Biotechnology Co., Ltd., product number FND500; Ad-mCherry-GFP-LC3B adenovirus was purchased from Biyuntian Biotechnology Co., Ltd., product number C3011- 1ml), etc.

[0043] Establishment of autophagy flow detection model: first, in 96-well plate with 5×10 4 Inoculate A549 cells per well, 100uL per well, and culture for 24h. Ad-mCherry-GFP-LC3B adenovirus was used to infect A549 cells. The virus stock solution was diluted 60 times with serum-free medium to infect A549 cells at 25uL / well for 2 hours. At ...

Embodiment 2

[0057] Example 2 The detection of tercitrusin in inducing autophagy of human lung cancer epithelial cells (A549)

[0058] Establishment of autophagy flow detection model: first, in 96-well plate with 5×10 4 Inoculate A549 cells per well, 100uL per well, and culture for 24h. Ad-mCherry-GFP-LC3B adenovirus was used to infect A549 cells. The virus stock solution was diluted 60 times with serum-free medium to infect A549 cells at 25uL / well for 2 hours. At the same time, a group of A549 cells without Ad-mCherry-GFP-LC3B adenovirus was set as a blank control. In a 96-well plate, DMEM medium containing 3% serum was added to each well, 75uL / well. Continue culturing, adenovirus infection for 24 hours, replace the medium with fresh DMEM medium containing 3% serum, and continue culturing for 24 hours.

[0059] Establishment of MRSA-infected A549 cell model: In addition to the blank control group, the methicillin-resistant Staphylococcus aureus MRSA bacterial fluid was used for infecti...

Embodiment 3

[0062] Example 3 The detection of teruzhenin in inducing autophagy of human lung cancer epithelial cells (A549)

[0063] Establishment of autophagy flow detection model: first, in 96-well plate with 5×10 4 Inoculate A549 cells per well, 100uL per well, and culture for 24h. Ad-mCherry-GFP-LC3B adenovirus was used to infect A549 cells. The virus stock solution was diluted 60 times with serum-free medium to infect A549 cells at 25uL / well for 2 hours. At the same time, a group of A549 cells without Ad-mCherry-GFP-LC3B adenovirus was set as a blank control. In a 96-well plate, DMEM medium containing 3% serum was added to each well, 75uL / well. Continue culturing, adenovirus infection for 24 hours, replace the medium with fresh DMEM medium containing 3% serum, and continue culturing for 24 hours.

[0064] Establishment of MRSA-infected A549 cell model: In addition to the blank control group, the methicillin-resistant Staphylococcus aureus MRSA bacterial fluid was used for infectio...

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Abstract

The embodiment of the invention discloses application of specnuezhenide in preparing an autophagy inducer or a pharmaceutical composition. By means of discovery of the autophagy inducing effect of thespecnuezhenide, the specnuezhenide is expected to the developed into the novel autophagy inducer to be used for disease treatment or auxiliary treatment, such as auxiliary treatment of bacterial infection diseases and auxiliary treatment of tumors.

Description

technical field [0001] The embodiments of the present invention relate to the field of medical technology, in particular to an autophagy inducer and its application. Background technique [0002] Autophagy refers to the process in which long-lived proteins and damaged organelles in cells are degraded through the lysosome pathway, which is a unique phenomenon in eukaryotic cells. Autophagy produced during high temperature, hypoxia, starvation and other stresses can help cells resist these adverse factors and play a cytoprotective role. However, excessive activation or inappropriate timing of autophagy can lead to cell death, that is, autophagic cell death. Existing studies have shown that the dysregulation of autophagy is closely related to diseases such as tumors, neurodegenerative diseases, cardiovascular diseases, and infections. [0003] In the event of stress, autophagy can prevent the accumulation of toxic or carcinogenic damaged proteins and organelles, and inhibit c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/337A61K33/24A61P35/00A61P35/02A61P31/04
CPCA61K31/337A61K31/7048A61K33/24A61P31/04A61P35/00A61P35/02A61K2300/00
Inventor 高亮亮
Owner 高亮亮
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