69results about How to "Promote autophagy" patented technology

The invention discloses an intestinal mucosa repair agent for weaned piglets. The repair agent is prepared from 0.5-2.5 parts by weight of tannic acid, 0.5-1.5 parts by weight of carvacrol, 0.5-1.5 parts by weight of L-carnitine compounds and 6-20 parts by weight of montmorillonite-loaded zinc oxide. Compared with feed which is singly added with the tannic acid, the carvacrol, the L-carnitine compounds or the montmorillonite-loaded zinc oxide, the intestinal mucosa repair agent provided by the invention can reduce the diarrhea rate of the piglets by 75% or above, increase the height of intestinal villi and significantly improve the intestinal absorption and barrier functions of the piglets. The four components have significant interactions in the aspects of promoting intestinal mucosal repair, improving mitochondrial function and promoting mitochondrial autophagy, and have a positive synergistic combination effect. Furthermore, montmorillonite has a controlled release effect on the tannic acid, the carvacrol and the L-carnitine compounds, thus enabling the tannic acid, the carvacrol and the L-carnitine compounds to be easily mixed with the feed and further forming a uniform dispersion system; the repair agent is convenient to use. The intestinal mucosa repair agent for the weaned piglets can significantly protect the mitochondrial function of the weaned piglets and promote theintestinal mucosa repair by means of the synergistic effect of all the components, thereby greatly improving the absorption and barrier functions of the intestinal mucosa.

The present invention relates to antibodies specific for human Beclin-1 protein phosphorylated at position Thr 119 and uses thereof. In particular, these antibodies are useful in diagnosing diseases associated with impaired autophagy including cancer and neurodegenerative diseases. The invention further relates to human Beclin-1 mutated at position 119 with a phospho-mimicking residue and uses thereof for treating cancer and neurodegenerative diseases.

The invention provides an application of USP33 as a medication target in preparation of drugs, and belongs to the field of protein engineering. According to the invention, the deubiquitination enzymeUSP33 interacting with Parkin is discovered by utilizing co-immunoprecipitation and mass spectrometry technologies, so that the USP33 is further discovered to be positioned on the outer membrane of mitochondria, and the E3 ligase Parkin can be modified by deubiquitination. When the USP33 is knocked down, Parkin transposition to mitochondria can be promoted; therefore, mitochondrial autophagy is enhanced to remove damaged mitochondria; USP33 regulates mitochondrial autophagy by influencing deubiquitination of Parkin, apoptosis of nerve cells under MPTP treatment can be inhibited by knocking down USP33, USP33 can be used as a medication target for preparing a medicine, and good application potential and value are achieved.

The invention discloses a compound (E)-1,3,3-trimethyl-2-(2-(4-methylbenzo[4,5]imidazo[1,2-a]pyridine-3-yl)vinyl)-3H-indolium-1- iodide in preparing a drug for inhibiting A549 lung cancer cell growthand promoting the apoptosis of A549 cells and autophagy, wherein the drug dose capable of effectively promoting A549 cell apoptosis and autophagy is 1-10 [mu]M, preferably 5-10 [mu]M. The compound isexpected to serve as an effective tool for inhibiting tumor growth and lay a foundation for developing therapeutic drugs for non-small cell lung cancer.

The invention discloses a culture medium additive for preparing autophagosome-type tumor vaccine. A freeze-dried powder preparation comprises one group of compositions, and the compositions comprise a proteasome inhibitor, an autophagy inducer, a lysosomal alkalifier, an excipient, and an antioxidant. The culture medium additive for preparing autophagy small-body-type tumor vaccine and a preparing method thereof have the remarkable advantages that the additive compositions comprise the proteasome inhibitor, the lysosomal alkalifier and the autophagy inducer which synthesize with one another, on one hand, a proteasome degradation pathway and an autolysosome degradation pathway of a tumour cell can be inhibited simultaneously so that tumour antigens can be preserved; on the other hand, autophagosomes are induced to take shape, thus a large quantity of tumour antigens are preserved in the autophagosomes, and then the autophagosomes-type tumor vaccine can be prepared by extracting the autophagosomes.

The invention discloses application of a TGF-beta 1 inhibitor in the preparation of drugs to treat diabetic bone healing disorder and belongs to the technical field of biopharmacy. A series of studiesby the inventor verifies that the TGF-beta 1 inhibitor can promote autophagy of diabetic bone marrow mesenchymal cells so as to inhibit cell senescence, thereby giving rise to the ability to promoteosteogenic differentiation. Therefore, the TGF-beta 1 inhibitor is applicable to the preparation of drugs to promote the osteogenic differentiation of diabetic bone marrow mesenchymal cells or drugs to promote diabetic bone healing. The problem is solved effectively herein that drugs to treat diabetic bone healing are absent in the prior art.

The invention provides an application of a mesenchymal stem cell exosome in preparation of preparations for promoting mitophagy. By preparing an exosome derived from the adipose mesenchymal stem cells, Western Blot and flow experiments at the cell level confirm that the regulation and control effect of AMSC-Exo on the mitophagy of parenchyma cells and immune cells; at the same time, in-vivo experiments confirm the effect of the AMSC-Exo on mitophagy pathway in liver tissue; and biological preparations or medicaments for promoting mitophagy are further prepared. Meanwhile, by modifying the AMSC-Exo, the effect for promoting mitophagy of the AMSC-Exo is further improved. The exosome can promote mitophagy and correct mitochondrial dysfunction; has mitophagy promoting effect on both parenchymacells and immune cells; can be used for preparing preparations or medicines for treating diseases related to mitochondrial dysfunction; and can further improve ability for promoting the mitophagy after specific modification.

The invention discloses an application of rapamycin in promotion of neural stem cell amplification. The application limitation that the rapamycin is only used as an immunosuppressor at present is overcome, the rapamycin can remarkably enhance the amplification capacity of neural stem cells, and the rapamycin can be used for treating Alzheimer's disease, Parkinson's disease, Huntington's disease, ischemic cerebral apoplexy, brain and spinal cord injury and other diseases.

The invention discloses a compound (E)-2-(4-piperidinylstyrene)-1,3,3-trimethyl-3H-indolium iodide, which inhibits the growth of A549 cells and promotes the growth of A549 cells. The application of apoptosis and autophagy drugs, wherein the dose of the drug that can effectively promote the apoptosis and autophagy of A549 cells is 1-10 μM. The present invention is (E)-2-(4-piperidinylstyrene)-1,3,3-trimethyl-3H-indolium iodide as an effective tool for studying the inhibition of mTOR activity, The foundation has been laid for the development of non-small cell lung cancer treatment drugs.

The invention provides Longya Lilium and Lithocarpus litseifolius cookies which are prepared from the following raw materials in parts by weight: 20 to 40 parts of Longya Lilium extract; 20-40 parts of lithocarpus litseifolius extract, 40-60 parts of wheat flour, 30-50 parts of oat flour, 10-15 parts of eggs, 5-10 parts of skim milk, 3-7 parts of honey, 3-6 parts of red dates, 1-3 parts of bakingsoda, 5-9 parts of xylooligosaccharide, 2-5 parts of tea polyphenol, 1-3 parts of a food preservatives and 10-15 parts of edible oil. The Longya Lilium and Lithocarpus litseifolius cookies prepared bythe method are crisp in taste and rich in milk flavor, have the health-care effects of lowering three highs, resisting oxidation, allergy and cancer, tranquilizing mind by nourishing the heart, nourishing yin, clearing away the heart-fire, maintaining beauty, keeping young, regulating body functions and the like by adding the Longya Lilium extract and Lithocarpus litseifolius extract, and are popular among consumers.