Application of rapamycin in promotion of neural stem cell amplification

A neural stem cell, rapamycin technology, applied in nervous system cells, animal cells, nervous system diseases, etc., to overcome application limitations, enhance the ability to expand, and promote the effect of proliferation

Pending Publication Date: 2022-04-19
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the rapamycin discovered by the inventors can be directly applied in vivo (because there are adult neural stem cells in the human brain), while the above-mentioned patent cannot use iPS directly in vivo to induce

Method used

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  • Application of rapamycin in promotion of neural stem cell amplification
  • Application of rapamycin in promotion of neural stem cell amplification
  • Application of rapamycin in promotion of neural stem cell amplification

Examples

Experimental program
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Embodiment 1

[0026] Example 1 The effect of rapamycin on the expansion of neural stem cells in vitro

[0027] 1. Rapamycin drugs

[0028] (1) Treatment of rapamycin: Rapamycin was purchased from Selleck Company, CAS number: 53123-88-9, molecular weight 914.18. Take 50 mg of rapamycin powder, add 0.5469 ml of dimethyl sulfoxide (DMSO), oscillate to fully dissolve, and mix well. The final concentration of this stock solution is 10 mM. Store at -20°C after aliquoting.

[0029] (2) Treatment of neural stem cells: The experiment used isolated and cultured rat embryonic neural stem cells. Take the third generation of neural stem cells, the cell density is 1×10 6 / ml, 24 hours after inoculation, add the prepared rapamycin to the culture medium to make the final concentration 50nM, mix gently, and place at 37°C, 5% CO 2 Continue culturing for 48 h in the cell culture incubator.

[0030] (3) Control group: the control group was a negative control, and the neural stem cells in the negative cont...

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Abstract

The invention discloses an application of rapamycin in promotion of neural stem cell amplification. The application limitation that the rapamycin is only used as an immunosuppressor at present is overcome, the rapamycin can remarkably enhance the amplification capacity of neural stem cells, and the rapamycin can be used for treating Alzheimer's disease, Parkinson's disease, Huntington's disease, ischemic cerebral apoplexy, brain and spinal cord injury and other diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the use of rapamycin in promoting the expansion of neural stem cells. Background technique [0002] The decrease in the number of neural stem cells is the main reason why neurodegenerative diseases or nerve damage are difficult to repair themselves. The number of neural stem cells in adults is limited, and it is difficult to be activated and expanded from a resting state to perform functions. The transplantation of neural stem cells after in vitro expansion provides a feasible candidate for these patients. In addition, if the proliferation of neural stem cells can be effectively promoted in vivo, it will bring bright prospects for the treatment of such diseases. Autophagy is a conserved catabolic mechanism that degrades redundant or damaged proteins or organelles to provide amino acids, nucleotides, fatty acids and energy needed for rapid cell growth. Autophagy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/439A61P25/00A61P25/28A61P25/16A61P25/14A61P9/10C12N5/0797
CPCA61K31/439A61P25/00A61P25/28A61P25/16A61P25/14A61P9/10C12N5/0623C12N2501/04
Inventor 吴丽颖李建巩生辉何云凌曹炎陈迎
Owner ACADEMY OF MILITARY MEDICAL SCI
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