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GOQDs-based pH-response-type bionic nano-preparation and preparation method and application thereof

A biomimetic nano and nano preparation technology, applied in the field of biomedicine, can solve problems such as reducing the reticuloendothelial system, and achieve the effects of increasing solubility, reducing dosage, and realizing controllable release.

Pending Publication Date: 2021-12-10
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although biomimetic nano-preparations can effectively reduce the clearance of nano-drugs by the reticuloendothelial system and enhance the targeting of plaque sites, it is only possible to further target nano-drugs to target cells and make them release drugs in response to microenvironmental stimuli at the disease site. , in order to more effectively kill target cells to improve the efficacy of drugs

Method used

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  • GOQDs-based pH-response-type bionic nano-preparation and preparation method and application thereof
  • GOQDs-based pH-response-type bionic nano-preparation and preparation method and application thereof
  • GOQDs-based pH-response-type bionic nano-preparation and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] (1) Synthesis of HA-M @ AT @ GP bionic nanometric

[0052] a. Preparation of bionic hybrid films (M)

[0053] The whole blood of C57BL / 6 mice was washed with PBS (pH 7.4) and plasma was removed at 3000 rpm for 5 min. The red blood cell was resuspended in 0.25 × PBS (pH 7.4) at 4 ° C for 6-8 h. The lysate was then centrifuged for 10 min for 10 min, and RBCM was obtained and washed with 0.25 × PBS (pH 7.4) until the supernatant became colorless. Collect wash clean RBCM. Preparation of macrophages (RAW264.7 cells) film is carried out in accordance with the description of the membrane protein extraction formulation. When the Raw264.7 cells in the cell culture dish were overlap, the cells were scraped with a cell blade, and 800 rpm was centrifuged for 5 min. The collected cells were washed with PBS (pH 7.4) at 4 ° C and then suspended in a membrane protein extractant A containing PMSF (1 mM). The mixture was lysed in an ice bath for 30 min, 4 ° C for 10 min (80 W, 0.3 s, 0.5 s...

Embodiment 2

[0070] A pH response type bionic nanometric agent based on GOQDS, and the EDC is added to 60 mg, and the addition amount of NHS crosslinking agent is 20 mg.

Embodiment 3

[0072] A pH response type bionic nanoform agent based on GOQDS, and Different Embodiments Differential oxide in grade oxide quantum dots were prepared by NH 2 -PEG 2000 -NH 2 The amount of added is 5 mg.

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Abstract

The invention belongs to the technical field of biomedicines and particularly relates to a GOQDs-based pH-response-type bionic nano-preparation and a preparation method and application thereof. The pH-response-type bionic nano-preparation is composed of PEGylated graphene oxide quantum dots, hydrophobic drugs, bionic hybrid membranes and phosphatidylated hyaluronic acid, wherein the hydrophobic drugs are loaded onto the PEGylated graphene oxide quantum dots, the bionic hybrid membranes are camouflaged on outer layers of the PEGylated graphene oxide quantum dots loaded with the hydrophobic drugs, and the phosphatidylated hyaluronic acid is modified on surfaces of the bionic hybrid membranes. The pH-response-type bionic nano-preparation can be used for effectively prolonging the cycle half-life period of the bionic nano-preparation in blood, and enhancing the targeting ability and the controllable release of the bionic nano-preparation in a diseased region.

Description

Technical field [0001] The present invention belongs to the field of biomedical technology, and more particularly to a pH response type bionic nanoform agent based on GOQDS and its preparation method and application thereof. Background technique [0002] As a disease in the global incidence and the highest mortality, the initial pathological mechanism of atherosclerosis (AS) is oxidized low-density lipoprotein, OX-LDL damage vascular endothelial cells lead to local inflammatory response . Damaged site secretes cytokines raised a single nuclear cells and divided into macrophages phagocytosis OX-LDL, and eventually formed AS plaque. With the progression of the disease, a large number of cholesterol aggregates in advanced AS plaque, so that macrophage autogenesis is damaged by stability and induces myocardial infarction. At present, AS is primarily used for statins such as atorvastatin (at). While existing treatment is effective, the incidence of cardiovascular disease is effectivel...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/61A61K31/40A61K47/52A61K47/46A61P9/10A61P39/06A61P29/00
CPCA61K47/6901A61K47/61A61K31/40A61K47/6923A61P9/10A61P39/06A61P29/00Y02A50/30
Inventor 姜怡邓张慧萍尤沛栋杨安宁马胜超
Owner NINGXIA MEDICAL UNIV
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