41results about How to "Has commercial application value" patented technology

The invention discloses a method for loading nitride to a graphite nano-sheet and an application of the graphite nano-sheet, relates to a method for loading nitride and an application of the method and aims at solving technical problems that existing nitride materials are large in size, high in production cost, easy to agglomerate and the like. The method includes step one, preparing expanded graphite; step two, dissolving a tungsten source, a molybdenum source, a vanadium source and a surface active agent in a solvent to prepare a disperse system; step three, injecting the disperse system into expanded graphite layers with the assistance of vacuum pressure; step four, evaporating the solvent to dryness; step five, placing a product obtained from step four in a crucible, and heating the crucible by using a high temperature impulse microwave technology to obtain an oxide / graphite nano-sheet complex; and step six, placing the oxide / graphite nano-sheet complex in a tube furnace, and feeding nitriding gases to obtain the nitride / graphite nano-sheet complex. The method is simple and mild in reaction conditions, particle sizes of obtained nitride are small, the nitride is evenly distributed on graphite sheet surfaces and in layers, and the graphite nano-sheet can be used in low platinum fuel cells after precious metals are loaded to the graphite nano-sheet.

The invention discloses a method for preparing a polyethylene-polyamine-modified biomass-based magnetic heavy metal adsorbent, and the method comprises the following steps: S1, magnetic glucose hydrothermal carbon is prepared by hydrothermal reaction of a glucose aqueous solution and a ferroferric oxide aqueous solution; S2, the magnetic glucose hydrothermal carbon, a polyethylene-polyamine compound and a condensation agent are mixed for reaction to obtain the modified hydrothermal-glucose-based magnetic heavy metal adsorbent. The adsorbent prepared by the method can efficiently remove heavy metals in water bodies, and is prepared from glucose as a raw material, the glucose is abundant in nature, wide in distribution and renewable, use of toxic chemicals is avoided, operation is simple, the low-toxicity polyethylene-polyamine compound as a solvent and a reactant is less in environmental pollution, and is suitable for industrial production. The product aminated magnetic glucose hydrothermal carbon can be widely used as a heavy metal adsorbent and an organic pollutant adsorbent and in preparation and synthesis of a magnetic solid catalyst.

The invention discloses a protection method of a lithium metal electrode. By use of a simple alloyage method, antimony trifluoride is utilized to form an artificial protective film on the surface of alithium cathode, so that polarization of the lithium cathode can be effectively lowered, and generation of dendritic crystals can be suppressed. According to the method, antimony trifluoride and lithium are mainly utilized for a reaction to generate the dense artificial protective film, wherein antimony trifluoride is dissolved in propylene carbonate to prepare a uniform solution first, pretreatment is performed on the surface of a lithium wafer to remove oxidized lithium on the surface, the lithium wafer is cut into a round wafer, the round wafer is immersed in the antimony trifluoride solution for a reaction to obtain a treated lithium electrode, and finally a symmetric battery is obtained through assembling. Through a low-concentration long-time reaction, a dense alloy layer can be generated on the surface of lithium metal. Through the method, the polarization of the lithium metal cathode is fully lowered, circulation stability is good, generation of lithium dendritic crystals is effectively suppressed, and the stable lithium metal cathode is obtained; and when the lithium metal cathode is applied to a lithium battery, the circulation performance and safety performance of the lithium battery can be improved, and potential commercial application value is obtained.

The invention discloses a preparation method of an organic small molecule crystal patterned array. The preparation method includes the following steps: 1) preparing a photoresist-based elastic patterned template by ultraviolet photolithography; and 2) preparing an aqueous solution or organic solution of organic small molecules by taking the photoresist-based elastic patterned template as an induction template and taking a target substrate as a cover plate, and preparing the organic small molecule crystal patterned array by using a capillary printing method based on a sandwich structure system.The preparation method of the organic small molecule crystal patterned array uses the capillary printing method, which is easy in implementation, chip and efficient, of inducing the growth of the organic small molecule crystal patterned array by the template to facilitate applications of devices.

The invention discloses a method for rapidly diagnosing shrunken-fruitsclerotiniose of mulberry. Through the discovered pathogen scleromitrula shiraiana specificity conserved DNA sequence, a specificity SCAR-PCR primer is obtained and a shrunken-fruitsclerotiniose of mulberry SCAR-PCR diagnosis and detection method is established. The method is high in sensitivity (the detection sensitivity is 1pg / [mu]L, high in specificity, convenient and rapid to use and accurate and reliable in detection result, can be used for the field diagnosis of the shrunken-fruitsclerotiniose of mulberry and the detection of the mulberry bacteria carrying condition, and also can be used for the diagnosis of the disease incubation period (the mulberries are infected but the symptoms do not appear) so as to forecasting the diseases in an early stage, take necessary control measures in time, safely and effectively prevent and control the disease and lighten or avoid the economic loss. According to the method, the product such as a detection kit for the shrunken-fruitsclerotiniose of mulberry can be researched and developed; the method is widely applied to the scientific research and production practice of the industries related to mulberries.

The invention relates to the technical field of pH fluorescent probes and particularly relates to a lysosome targeted pH fluorescent probe for monitoring cell autophagy as well as preparation and application of the lysosome targeted pH fluorescent probe. The preparation method comprises the following steps of dissolving 2-(2-aminoethyl)-3',6'-bis(diethylamino) spiro [isoindole-1,9'-xanthan]-3-ketone and 2-(2-methoxyethoxy)4-methyl benzene sulfonic acid ethyl ester in N, N-dimethylformamide, and carrying out heating reflux to obtain a crude product; and removing a solvent from the crude product, and separating through a silica gel column to obtain a pure product. Cytotoxicity tests show that the probe has almost no toxic or side effect on cells, a cell co-localization experiment determinesthat the probe can specifically target a cell lysosome, and a laser confocal microimaging experiment shows that the probe has good cell membrane permeability and can perform high-sensitivity detectionon pH change in the lysosome. The probe provided by the invention can monitor the autophagy process of the cells by detecting the change of pH in the lysosome.

The invention discloses a carbazole derivative MCAB and preparation and application methods thereof. The carbazole derivative MCAB is named as 4-(3-(9-(2-(2-methoxyethoxy)ethyl)-9H-carbazol-3-yl)-3-acryloyl)benzaldehyde, or MCAB, for short. The preparation method of the carbazole derivative MCAB comprises dissolving 1-(9-(2-(2-methoxyethoxy)ethyl)-9H-carbazol-3-yl)ketone and benzaldehyde into ethyl alcohol, then adding piperidine and performing heating and refluxing to obtain a crude product; removing solvent from the rough product, and performing silica gel column separation to obtain a purified product. The MCAB can serve as a probe and take alpha, beta-unsaturated acetylcarbazole derivatives as fluorophore masterbatch and cysteine (Cys) reaction sites to induce nucleophilic addition ofalpha, beta-unsaturated ketone through sulfydryl and further to lead fluorescence enhancement, thereby achieving Cys specific detection, being high in selectivity and higher in activity of reaction with Cys compared with other biological mercaptans. By means of laser confocal fluorescence imaging technology, the carbazole derivative MCAB can highly sensitively detect change of Cys in lysosomes andaccordingly have a broad application prospect in the field of biological molecular detection.

The invention relates to the technical field of lithium batteries, and discloses a cobalt-free lithium-rich manganese-based positive electrode material, a composite positive electrode sheet and a lithium ion battery, wherein the chemical formula of the cobalt-free lithium-rich manganese-based positive electrode material is xLi2MnO3.(1-x)LiMeO2+a, x is greater than 0 and less than 1, a is greater than or equal to 0 and less than 1, and Me is selected from more than one of Mn, Ni, Al, Mg, Ti, B, F, Y and La. When the cobalt-free lithium-rich manganese-based positive electrode material is used for preparing a lithium ion battery, the preparation process is simple, the energy consumption is low, the cost is low, the industrialization is easy, and the prepared battery has the advantages of highfirst effect, good rate capability, slow voltage attenuation in the cycle process and good cycle stability, and has commercial application value.

The invention relates to the technical field of pH fluorescent probes, in particular to a rhodamine pH fluorescent probe for monitoring mitochondrial autophagy and preparation and application thereof.The invention aims to solve the problems that many fluorescent probes for detecting lysosome pH change are not suitable for long-time monitoring of cells, and lysosome targeting pH fluorescent probespractically used for monitoring mitochondrial autophagy are few and the like. The invention discloses a preparation method, and the method comprises the following steps: dissolving an N-p-toluenesulfonic acid ethyl ester derivative of rhodamine B and N-aminomorpholine in dichloromethane, carrying out heating reflux, carrying out cooling to room temperature, carrying out reduced pressure rotary evaporation to remove a solvent so as to obtain a crude product, and carrying out silica gel column chromatography purifying on the crude product so as to obtain a faint yellow solid which is the pH fluorescent probe. The fluorescent probe has good cell film permeability, can mark lysosome in a targeted manner, realizes visual monitoring of the mitochondrial autophagy process by detecting the changeof the pH of the lysosome, and has potential application prospects in lysosome pH physiology and pathology research.

The invention relates to a manufacturing method of electrochromic cloth. According to the method, an all-solution preparing and processing process is adopted, and electrodes are membranes formed by ITO electro-conduction slurry after being dried, in a planar cross distribution structure. The manufacturing method is simple, the cost is low, the prepared electrochromic cloth is thin, light, resistant to flexion and high in practicability, subsequent processing is facilitated, and large scale production is easy to achieve.

The invention discloses a fast-charging type cobalt-free high-nickel concentration gradient core-shell structure lithium ion battery ternary positive electrode material and a preparation method thereof. The chemical formula of the positive electrode material is Li [NixMn1-x-yWy] O2 (at) mLi2ZrO3, x and y are mole numbers, x is larger than or equal to 0.8 and smaller than 1, y is larger than 0 and smaller than or equal to 0.05, m is mass fraction, and m is larger than 0 and smaller than or equal to 3000 ppm. The preparation method comprises the following steps: adding a first salt solution containing nickel, manganese and tungsten, a sodium hydroxide solution and an ammonia water solution into a reaction kettle according to a certain stoichiometric ratio, and reacting to form a precursor inner core; adding second salt solutions containing nickel, manganese and tungsten in different stoichiometric ratios into the reaction kettle, fully reacting, and then carrying out centrifugal washing, drying and screening to remove iron, so as to obtain a spherical precursor with the chemical formula of [NixMn1-x-yWy] (OH) 2; and sufficiently mixing the precursor powder with a zirconium source and a lithium source through a high-energy mixer, and roasting to finally obtain the fast-charging cobalt-free high-nickel concentration gradient core-shell structure lithium ion battery ternary positive electrode material. The positive electrode material disclosed by the invention has a relatively good crystal structure, and a battery prepared by taking the positive electrode material as the positive electrode material has excellent electrochemical performance; the preparation method is simple, controllable and low in cost, and has commercial application value.

The invention discloses a signal screening method of a touch device. The method comprises the following steps: acquiring a touch signal; acquiring signal intensity values corresponding to a first induction area, a second induction area and a third induction area according to the touch signal, wherein the second induction area is positioned between the first induction area and the third induction area; and if the signal intensity value of the second induction area is less than the signal intensity values of the first induction area and the third induction area, removing the touch signal. The signal screening method can remove noise of the signal so as to improve the accuracy and the stability of a capacitance touch device.

The invention relates to a catalyst for synthesizing diethyl oxalate by CO gas-phase coupling of ethyl nitrite and a preparation method thereof. The catalyst comprises a carrier, an active component palladium and a metal auxiliary agent iron, wherein the carrier is commercial hollow columnar [alpha]-Al2O3, the content of the active component palladium accounts for 0.1-1.0 wt% of the weight of thecarrier, and the content of the metal auxiliary agent iron accounts for 0.1-1.0 wt% of the weight of the carrier. According to the preparation method, a commercial hollow columnar carrier is adopted,and vegetable tannin and / or anhydrous dextrose and / or polyvinylpyrrolidone are / is added, so that palladium metal nanoparticles in the catalyst are smaller, the dispersity is higher, and the palladiumloading capacity is lower. The catalytic activity of the catalyst is basically improved by more than two times, the cost of the catalyst is reduced by more than four times, the prepared catalyst carrier is suitable for filling of a fixed bed with high space velocity by taking industrialization as a reference, and more precious experience is provided for industrialization of diethyl oxalate production.

The invention provides a preparation method of a fish polypeptide with uniform molecular weight distribution. The preparation method comprises following steps: fish skin is subjected to pretreatment, and multistage tandem enzymolysis for a period of time; a gelatin crude product is obtained via enzyme inactivation; and the fish polypeptide is obtained via extraction; wherein multistage tandem enzymolysis is designed to be 4 to 6 stages, temperature of each stage of enzymolysis is controlled to be 25 to 55 DEG C, pH value is controlled to be 6 to 9, temperature is increased gradually from the first stage to the next stages, and pH value is reduced gradually. Molecular weight distribution of the fish polypeptide is uniform; application range is wide; the fish polypeptide can be used for preparing cosmetics, drugs, and health food; and absorption effect by human body is excellent.

The invention discloses a polysiloxane hybrid quantum dot nano material, which comprises quantum dots and polysiloxane, wherein the quantum dots are connected with the polysiloxane through chemical bonds to form nano particles with a particle size of 1-20 nm, and the quantum dots comprise silicon quantum dots and / or carbon quantum dots. The invention also provides a polysiloxane hybrid quantum dotnano material and application in an LED light source. According to the invention, the polysiloxane hybrid quantum dot nano material provided by the invention has excellent light, heat and chemical stability, overcomes the problem of poor stability of semiconductor quantum dots emitting blue light, and can be used as a blue light component in an electroluminescent material; and the service life ofa QLED device prepared from the polysiloxane hybrid quantum dot nano material at least reaches 80000 hours, and the QLED device has a commercial application value.

The invention provides a method for quickly preparing precious metal parts at a low temperature. The method comprises the steps as follows: designing a die based on the overall dimension of a pre-formed precious metal parts; feeding the raw material which is the precious metal powder into the die; carrying out press molding at 10 to 100MPa firstly; and then transferring into an electric spark ion sintering device; heating to reach the temperature of sintering by controlling the heating rate under a vacuum or common protective atmosphere condition; and keeping the temperature for a certain time so as to obtain the formed precious metal parts. Compared with the existing preparation method of precious metal parts, the method can prepare the precious metal parts by once sintering; and by adopting the method, the sintering temperature can be greatly reduced, the preparation speed can be raised, and the obtained parts is uniform and has high density; and the method is a simple and practicable preparation method which has low cost and has commercial application value.

The invention provides a method for quickly preparing precious metal parts at a low temperature. The method comprises the steps as follows: designing a die based on the overall dimension of a pre-formed precious metal parts; feeding the raw material which is the precious metal powder into the die; carrying out press molding at 10 to 100MPa firstly; and then transferring into an electric spark ion sintering device; heating to reach the temperature of sintering by controlling the heating rate under a vacuum or common protective atmosphere condition; and keeping the temperature for a certain time so as to obtain the formed precious metal parts. Compared with the existing preparation method of precious metal parts, the method can prepare the precious metal parts by once sintering; and by adopting the method, the sintering temperature can be greatly reduced, the preparation speed can be raised, and the obtained parts is uniform and has high density; and the method is a simple and practicable preparation method which has low cost and has commercial application value.

A full magnetic energy engine comprises a driving device, a magnetism storage mechanism, a magnet piston mechanism and a connecting rod crank shaft mechanism, wherein the driving device is used for driving the magnetism storage mechanism to rotate, and the magnetism storage mechanism is used for driving the magnet piston mechanism to move and provided with a center shaft. Work-doing permanent magnets for doing work are fixed on the left sides and the right sides of both the front end and the rear end of the center shaft, Magnetic shafts of the work-doing permanent magnets are parallel to the center shaft, poles of the work-doing permanent magnets of the left sides and poles of the work-doing permanent magnets of the right sides are reverse, and poles of the work-doing permanent magnets of corresponding sides of the front end and the right end are reverse. The magnet piston mechanism is used for generating output power and provided with a magnet storage chamber. A permanent magnet with a magnetic shaft parallel to the center shaft is fixed in the magnet storage chamber, two suspending connecting bridges are hinged to the top surface of the magnet storage chamber, and connecting rod shafts are fixed on the left side and the right side of the magnet storage chamber. The full magnetic energy engine has the advantages that the full magnetic energy engine utilizes energy in the permanent magnets to do work, can reduce dependence on fossil energy, does not consume the fossil energy, does not generate environmental pollution caused by greenhouse gas, and is low in cost, simple in structure and high in business application value.

The invention discloses a composite solid electrolyte material and a preparation method and application thereof. The electrolyte material has an amorphous and heterojunction mixed interface. The chemical composition is (xLiBH4-yLiI)-zBN, x, y and z are molar ratios, 1 < = x < = 5, 0 < = y < = 5, and 1 < = z < = 5. The preparation method comprises the following steps: in an inert gas atmosphere, firstly carrying out pre-ball milling on a certain amount of h-BN, then mixing the h-BN with xLiBH4-yLiI according to a certain molar ratio, then carrying out ball milling, and then carrying out heat treatment under a certain hydrogen pressure. The material disclosed by the invention has excellent room-temperature ionic conductivity, a wide electrochemical window, high lithium ion mobility, almost negligible electronic conductivity, excellent electrode compatibility, extremely strong lithium dendrite inhibition capability and excellent all-solid-state battery performance. The material is a lithium ion conductor with excellent performance at room temperature (T is less than 100 DEG C), and can be used as a solid electrolyte of an all-solid-state lithium ion battery.

The invention discloses a self-supporting high-molecular polymer membrane as well as a preparation method and application thereof. The polymer membrane is prepared by taking aramid nano-fibers as a connector, blending and doping a high-molecular polymer with the aramid nano-fibers, and performing suction filtration, and based on the total mass of the membrane, the mass percentage of the high-molecular polymer in the polymer membrane is 45.6 wt%-92.3 wt%. The method comprises the following steps of putting Kevlar fibril and potassium hydroxide into a container filled with a dimethyl sulfoxide solvent, and stirring to form an aramid nano-fiber dispersion liquid, adding the aramid nano-fiber dispersion liquid and a high-molecular polymer into a container, and stirring, and carrying out vacuum filtration treatment on the uniformly stirred dispersion liquid, and finally drying and stripping to obtain the salinity gradient power generation high-molecular polymer membrane. The required high-molecular polymer membrane is obtained by adopting a vacuum filtration method, the method and the process are simple and easy to implement, the cost is low, and large-scale preparation can be realized; and the prepared high-molecular polymer membrane is high in energy conversion efficiency and has certain commercial application value.

The invention relates to the field of enzyme engineering, discloses fructosyl peptidyl oxidase and an encoding gene thereof. The amino acid sequence of fructosyl peptidyl oxidase is shown in SEQ ID No:1. The invention also discloses a recombinant vector with the gene and a transformant containing the recombinant vector. The invention also discloses applications of fructosyl peptidyl oxidase, the gene, the recombinant vector or the tansformant to detection of glycosylated hemoglobin by an enzyme method. In addition, the invention also discloses a method for enhancing specificity of fructosyl peptidyl oxidase. The method comprises a step for replacing arginine at the 62th position of fructosyl peptidyl oxidase derived from Eupenicillium terrenum into the other amino acid, and the other amino acid is lysine, alanine, asparagine, glutamine, phenylalanine, valine, isoleucine, histidine, leucine, serine, methionine or proline. Fructosyl peptidyl oxidase with obviously improved specificity of enzyme activity is obtained, so that the business application values of the enzyme are increased.

The invention proposes an imidazo[1,2-a]pyridine ratiometric pH fluorescent probe and a preparation method thereof, and the probe is used for the determination of extremely acidic pH changes in living cells and Escherichia coli. The preparation method is as follows: under the protection of an inert gas, N, N-dimethylbenzaldehyde or its derivatives, 4-chloroaniline and sodium bicarbonate are dissolved in methanol, and heated to reflux to obtain a crude product; and 2-(4-methoxyphenyl)-7-methylimidazo[1,2-a]pyridine dissolved in N,N-dimethylformamide, with potassium tert-butoxide as alkaline reagent, heating Reflux reaction; separated by silica gel column. The probe exhibits ratiometric emission fluorescence characteristics under extremely acidic conditions, and at the same time has the ability to H + It has the advantages of high sensitivity, good selectivity and large Stokes shift.

The invention discloses a method for separating and purifying yak stomach lysozyme. The method comprises the following steps: (1) homogenizing to obtain homogenate; (2) carrying out coarse purification to obtain a coarse purified solution; (3) carrying out CM-Sepharose FF column chromatography to obtain a chromatographic solution; (4) carrying out Sephadex G-75 column chromatography to obtain a yak stomach lysozyme solution; and (5) and lyophilizing to obtain yak stomach lysozyme. By using the method disclosed by the invention, the pure yak stomach lysozyme can be separated from yak gastric mucosal tissue, has high enzyme activity, wide application range and antibacterial effect and can be used as an antibacterial agent. Meanwhile, the method is high in yield and simple and convenient to operate and has industrial application values.

The invention discloses a method for loading nitride to a graphite nano-sheet and an application of the graphite nano-sheet, relates to a method for loading nitride and an application of the method and aims at solving technical problems that existing nitride materials are large in size, high in production cost, easy to agglomerate and the like. The method includes step one, preparing expanded graphite; step two, dissolving a tungsten source, a molybdenum source, a vanadium source and a surface active agent in a solvent to prepare a disperse system; step three, injecting the disperse system into expanded graphite layers with the assistance of vacuum pressure; step four, evaporating the solvent to dryness; step five, placing a product obtained from step four in a crucible, and heating the crucible by using a high temperature impulse microwave technology to obtain an oxide / graphite nano-sheet complex; and step six, placing the oxide / graphite nano-sheet complex in a tube furnace, and feeding nitriding gases to obtain the nitride / graphite nano-sheet complex. The method is simple and mild in reaction conditions, particle sizes of obtained nitride are small, the nitride is evenly distributed on graphite sheet surfaces and in layers, and the graphite nano-sheet can be used in low platinum fuel cells after precious metals are loaded to the graphite nano-sheet.

The invention discloses a device and method for testing the fatigue characteristics of a gating tube, and the device comprises a voltage dividing element and a counter, the voltage dividing element isconnected with a to-be-tested gating tube, and is used for dividing the voltage of the to-be-tested gating tube in a testing process; and the counter is connected with the gating tube to be detectedand is used for detecting the voltage and / or current change of the gating tube to be detected. A gating tube to be tested is used as a constituent part of an oscillator; the structure of the device issimpler, and complex circuit components such as a pulse generator and a judging circuit are omitted; in addition, periodic voltage and / or current oscillation is realized on the basis of the characteristics of the gating tube, so that the test period is shorter, the test time is saved, and the device is extremely simple in composition and extremely low in cost and has important commercial application value.

The invention discloses a method for rapidly diagnosing shrunken-fruitsclerotiniose of mulberry. Through the discovered pathogen scleromitrula shiraiana specificity conserved DNA sequence, a specificity SCAR-PCR primer is obtained and a shrunken-fruitsclerotiniose of mulberry SCAR-PCR diagnosis and detection method is established. The method is high in sensitivity (the detection sensitivity is 1pg / [mu]L, high in specificity, convenient and rapid to use and accurate and reliable in detection result, can be used for the field diagnosis of the shrunken-fruitsclerotiniose of mulberry and the detection of the mulberry bacteria carrying condition, and also can be used for the diagnosis of the disease incubation period (the mulberries are infected but the symptoms do not appear) so as to forecasting the diseases in an early stage, take necessary control measures in time, safely and effectively prevent and control the disease and lighten or avoid the economic loss. According to the method, the product such as a detection kit for the shrunken-fruitsclerotiniose of mulberry can be researched and developed; the method is widely applied to the scientific research and production practice of the industries related to mulberries.

The invention discloses a method for preparing a trimethylamine gas sensor from a flower-shaped NiO-In2O3 material, and relates to a method for preparing a sensor from a composite material, and the gas sensor is composed of an alumina ceramic tube, a nickel-cadmium alloy heating wire, a platinum wire, a gold electrode and a NiO-In2O3 composite sensitive material. A simple one-step hydrothermal method is adopted, the optimal loading capacity and the optimal composite structure of the In2O3 surface are finally determined by adjusting the molar content of the nickel source in the composite material, and the method has the advantages of being simple in preparation process, low in cost, environmentally friendly and suitable for industrial production. The NiO-In2O3 composite material with the flower-like structure prepared by the invention shows good sensitive characteristic in trimethylamine gas detection, has the advantages of higher sensitivity, good reproducibility and stability, high selectivity, lower detection limit and the like, and is simple in synthesis method, low in cost, small in size, suitable for industrial mass production and wide in application prospect. Wide application prospects are shown in the field of high-performance gas sensors.

The invention relates to a preparation methodof a capillary biosensor based on capillarity, which comprises the following steps: loading a surfactant on an aminated mesoporous silica ball with positive electricity on the surface, sealing the mesoporous of the silica ball by using a nucleic acid aptamer with a certain base length, and modifying the sealed silica ball on the inner wall of a capillary in an adsorptive manner, and preparing the capillary sensor with target identification. When a target to-be-detected object exists, the nucleic acid aptamer on the meso-porous silicon ball can be specifically combined with the target to be detected and is separated from the surface of the meso-porous particle, so that a surfactant loaded in the meso-pores is released. As the surfactant is dispersed in the aqueous solution to reduce the surface tension of water, the liquid level of a water column rising through the capillarity in the capillary tube is reduced, and the concentration of a target object can be quantified by utilizing the change of the height of the water column in the capillary tube. The sensor does not need complex instruments, is simple and rapid to operate, low in cost and wide in applicable target object range, can be used for detecting toxins, small molecules, ions, proteins and the like, and has commercial application value.

The invention provides a chlorine-resistant and anti-pollution soft water nanofiltration membrane and a preparation method thereof. The nanofiltration membrane includes a polysulfone base membrane, and the polysulfone base membrane is sequentially covered with a polypiperazine amide layer and a surface modification layer; Wherein, the surface modification layer is formed by reacting thiourea dioxide and polyvinyl alcohol on the surface of the polypiperazine amide layer. The nanofiltration membrane has the advantages of the traditional salt separation nanofiltration membrane, and has excellent hardness removal ability, chlorine resistance and anti-pollution performance. The modified nanofiltration membrane has significantly improved chlorine resistance and anti-pollution performance, and can maintain its High calcium chloride retention rate. The nanofiltration membrane prepared by the invention has broad application prospects in the field of advanced treatment of municipal drinking water where the water quality is relatively hard or groundwater is used as the water source.