A kind of separation method of yak stomach lysozyme

A lysozyme and yak technology, which is applied in the field of separation of yak stomach lysozyme, can solve problems such as no separation and purification of yak stomach lysozyme, and achieves the effects of good industrial application prospects, high yield and easy operation.
CN104371987BActive Publication Date: 2017-01-25SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHWEST UNIVERSITY FOR NATIONALITIES
Publication Date
2017-01-25

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Abstract

The invention discloses a method for separating and purifying yak stomach lysozyme. The method comprises the following steps: (1) homogenizing to obtain homogenate; (2) carrying out coarse purification to obtain a coarse purified solution; (3) carrying out CM-Sepharose FF column chromatography to obtain a chromatographic solution; (4) carrying out Sephadex G-75 column chromatography to obtain a yak stomach lysozyme solution; and (5) and lyophilizing to obtain yak stomach lysozyme. By using the method disclosed by the invention, the pure yak stomach lysozyme can be separated from yak gastric mucosal tissue, has high enzyme activity, wide application range and antibacterial effect and can be used as an antibacterial agent. Meanwhile, the method is high in yield and simple and convenient to operate and has industrial application values.
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Description

technical field

[0001] The invention relates to a method for separating yak stomach lysozyme. Background technique

[0002] Lysozyme is a basic protein, which is widely distributed in animals, plants and microorganisms, and is expressed in mammalian plasma, trachea, intestinal tract, stomach, kidney and liver tissue cells. Lysozyme lyses bacteria by disrupting the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, resulting in a structural change in the cell wall. Lysozyme can be used as a potential substitute for antibiotics in the fields of food, bioengineering, medical treatment, and livestock and poultry feed due to its antibacterial properties, non-toxicity, and side effects. From the perspective of livestock disease control, lysozyme is used as an additive in feed, which can effectively prevent livestock diarrhea, and at the same time provide nutrition and intestinal protection, thereby reducing the death of livestock caused b...

Claims

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