Method for rapidly breeding corn haploid induction line, and application of method

A haploid induction system and haploid technology, applied in the biological field, can solve the problems of limited breeding cycle, inability to identify haploids, long field test work, etc., to promote development, improve haploid breeding efficiency, The effect of reducing test workload

Active Publication Date: 2020-12-01
CHINA AGRI UNIV
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Problems solved by technology

Heavy field tests and long breeding cycles greatly limit the breeding process of maize haploid induction lines
The haploid breeding technology can greatly shorten the breeding cycle of pure lines and reduce human and economic costs. However, the haploid induced lines of maize currently used in practice all carry the R1-nj marker, and the embryos and endosperms of the grains are purple. Conventional methods cannot identify its haploid

Method used

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  • Method for rapidly breeding corn haploid induction line, and application of method
  • Method for rapidly breeding corn haploid induction line, and application of method
  • Method for rapidly breeding corn haploid induction line, and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Breeding Maize Haploid Inducer Line

[0048] 1. Construction of basic materials for induction system

[0049] In Hainan in the winter of 2019, maize haploid induction lines CAU3 (induction rate about 10%) and CAU6 (induction rate about 12%) were planted, with CAU3 as the female parent and CAU6 as the male parent to form a hybrid combination CAU3 / CAU6, Harvest F 1 Seed (F of CAU3 / CAU6 1 generation). The F 1 Seeds are the base material.

[0050] 2. Hybridization induction to obtain candidate material groups containing haploid

[0051] Planting CAU3 / CAU6 F in Beijing in the spring of 2020 1 The generation was used as the female parent (induced material), and the inducible line CAU5 (induction rate was about 10%) was used as the male parent for induction. After 15 days after pollination, the hybrid immature embryos were peeled off and placed on MS solid medium for culture to obtain single Ploid candidate material group (haploid immature embryos to be ident...

Embodiment 2

[0096] Example 2 Distinguishing Inducible Line Haploids Based on Oil Content

[0097] 1. Induction of the haploid of the inducer line and determination of oil content

[0098] In the spring of 2018 in Beijing, maize haploid induction lines CAU3 (induction rate of about 10%) and CAU6 (induction rate of about 12%) were planted, with CAU3 as the female parent and CAU6 as the male parent to form a hybrid combination CAU3 / CAU6, Harvest F 1 Seed, its oil content is about 3%.

[0099] Hainan in the winter of 2019, planting CAU3 / CAU6 F 1 The generation was used as the female parent (induced material), and the high-oil inducible line CHOI4-1 (induction rate was about 12%, oil content was about 8%) was used as the male parent for hybridization, and 1000 hybrid seeds were selected, and the low-field NMR Sorting instrument (model: nuclear magnetic resonance oil and water content analyzer PQ001-20-025V, Suzhou Numei Analytical Instruments Co., Ltd.) measures the oil content of the grain...

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Abstract

The invention discloses a method for breeding a corn haploid induction line. An identification method for the colored embryo haploid of the induction line is developed so that haploid identification of the corn haploid induction line can be realized, thus doubling is conducted, an inducible line DH line with high inductivity, high identification efficiency and excellent agronomic traits can be screened out from the expression of four aspects of molecular marker-assisted selection inducible genes, inductivity, haploid identification efficiency and the agronomic traits, and the excellent haploidinducible line can be obtained within 1-2 years in the whole process. According to the method capable of rapidly breeding the corn haploid inducible line, the breeding process of the haploid inducible line is accelerated, a feasible scheme for solving the problems of long breeding period and large workload of the corn haploid induction line is provided, the breeding period of the haploid induction line can be greatly shortened, the workload of test of each generation is decreased, and the haploid breeding efficiency is improved, thus development of a corn haploid breeding technology is promoted, and the method has important significance.

Description

technical field [0001] The invention relates to a method for rapid breeding of maize haploid induction lines in the field of biotechnology and its application. Background technique [0002] Maize haploid breeding technology, as one of the three modern biotechnologies, is widely used in breeding and scientific research. Haploid-inducing lines are the first condition for realizing haploid breeding technology, so a lot of research has been done on haploid-inducing lines in recent years. The existing induction lines are all derived from Stock 6, and its induction rate is 3.2% (Coe, 1959). With the artificial test breeding and molecular marker-assisted selection, a large number of high-frequency induction lines of maize haploid have been bred, mainly including CAU series, CHOI series, UH series, RWS / K series, TAIL series and PHI series (Ren et al, 2017), the haploid induction rate increased by about 10%. The continuous improvement of the haploid induction rate has solved the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 陈绍江陈琛刘晨旭肖子健钟裕焦炎炎陈明李金龙
Owner CHINA AGRI UNIV
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