37 results about "Protein i" patented technology

The present invention is continuous water milling production process of extracting buckwheat starch, buckwheat protein, flavone and dietary fiber. Buckwheat is water soaked, milled into milk and separated in a horizontal sieve to obtain buckwheat slurry and buckwheat dreg; buckwheat slurry is separated continuously to obtain buckwheat starch-I, buckwheat starch-II, buckwheat protein-I, buckwheat protein-II and buckwheat protein-III; and buckwheat dreg is separated to obtain buckwheat protein-IV, flavone and dietary fiber. The present invention obtains eight kinds of product with high additional value from buckwheat, and the production process has less investment, high production efficiency, low cost, no environmental pollution and other advantages.

The invention discloses a weight assembly clustering method for excavating a protein complex. The method comprises the following steps: inputting a protein interaction network to produce an undirected graph, selecting m clustering methods to be applied to the network to obtain m clustering results; rebuilding characteristic networks for the base clustering results to obtain m characteristic networks, wherein the m characteristic networks correspond to m characteristic matrixes; combing the m characteristic matrixes to obtain a combined matrix W, wherein uq refers to weights of the qth characteristic network, and uq is larger than or equal to 0, the combined matrix W corresponds to a new network, and elements Wi and j are used for measuring the similar degree of the protein i and the protein j in the new network; excavating the clusters in the new network through adopting a Bayes non-negative matrix factorization algorithm; integrating the weight studying and the complex discovering into an optimal object, so that the weight is optimized through the clustering result, otherwise, the clusters are guided by the weight results; and obtaining the final protein complex excavating result after the optimizing is finished.

CTnI synthetic polypeptide antibody, its production and use are disclosed. The process is carried out by synthesizing polypeptide by 98-110 amino-acid at N end of amino-acid sequence RQLHARVDKVDEE, taking it as partial antigen to connect with protein carrier, preparing artificial immunogen, immunizing for animal, inducing organism effectively, generating immune response to obtain antibody, specific combined reacting cTnI synthetic polypeptide antibody with natural myoepicardial protein I molecule and coating cellulose nitrate film by the antibody, and reducing by tri-sodium citrate to obtain the final product. It's fast and has better sensitivity and specificity, and it can be used for acute myocardial infarction early diagnostic technology.

The invention discloses a method for preparing walnut oil by performing emulsion breaking on a walnut oil body by a water method, and belongs to the technical field of processing of plant oil and proteins. The walnut oil and walnut proteins are prepared by preparing walnut pulp, the walnut oil body, walnut oil I, walnut protein I, the walnut oil body in a heavy phase and walnut protein II, and combining the prepared materials. According to the method, no thermal treatment is carried out in the process of preparing the walnut pulp (or an extract of a walnut water phase), so that the walnut proteins and other components cannot be affected by thermal treatment; a light phase (the walnut oil body) part obtained by centrifugation can be subjected to efficient emulsion breaking by using the method, so as to obtain clear and fragrant walnut oil; by condition optimization, 98 percent or above of oil, obtained by centrifugation, in the walnut oil body can be released. The method is simple in process, and a centrifugation machine is used as main equipment; no enzyme preparation or organic solvent is used, so that the preparation process is environment-friendly and efficient.

Methods of activating a signaling cascade comprising, introducing leptin and / or a cytokine to a receptor complex comprising gp 130, optionally in combination with a compound acting on adenylate cyclase or acting on one or more downstream targets of adenylate cyclase, thereby inducing genes in neuro-endocrine cells or cells of neuro-endocrine origin. Two distinct gene-sets are induced, immediate early response genes (STAT-3, SOCS-3, Metallothionein-II, the serine / threonine kinase Fnk and the rat homologue of MRF-1), and late induced target genes (Pancreatitis Associated Protein I, Squalene Epoxidase, Uridinediphosphate Glucuronyl Transferase and Annexin VIII). Strong co-stimulation with the adenylate cyclase activator forskolin was shown with respect to late induced target genes. Transcripts encoding Leptin Induced Protein I (LIP-I) and Leptin Induced Protein II (LIP-II) were identified; however, no forskolin co-stimulatory effect was observed. It is also demonstrated that leptin modulates in vivo expression of MT-II, Fnk and Pancreatitis Associated Protein I genes.

The invention relates to a method for preparing sesame oil through water-method demulsification of a sesame oil body and belongs to the technical field of processing of vegetable fat and proteins. According to the method, the sesame oil and sesame proteins are prepared through the preparation of sesame slurry, the sesame oil body, sesame oil I and sesame protein I, the separation of the sesame oil body in a heavy phase and the preparation and combination of sesame protein II. The thermal processing is omitted during the preparation of the sesame slurry (or referred as to a sesame water phase extract), so that the influences caused by the thermal processing to the sesame proteins and other components are avoided; by carrying out efficient demulsification on a light phase (the sesame oil body) obtained through centrifugation, the clear and mellow sesame oil can be obtained; and by virtue of condition optimization, more than or equal to 98% of oil in the sesame oil body can be released through centrifugation. The method has the beneficial effects that the process is simple, and main equipment is a centrifugal machine; and no any enzyme preparation is used, and no organic solvent is used, so that the preparation process is environmentally friendly and efficient.

The invention discloses a microfludic immunodetection chip and method of cardiac troponin protein I. In the microfludic immunodetection chip, a sample storage pool, a reaction detection pool and a waste-liquid pool are arranged on a microchannel main path in sequence; one end of the microchannel main path is provided with a sampling port, and the other end of the microchannel main path is connected with a first pump; one end of a microchannel branch path is communicated with a water storage pool by a second pump, and the other end of the microchannel branch path is connected with the microchannel main path between the reaction detection pool and the waste-liquid pool; the microchannel branch path is provided with a reagent B filling pool and a high-value calibration pool in sequence; a low-value calibration pool is connected with the high-value calibration pool in parallel; the microchannel branch path between the reagent B filling pool and the high-value calibration pool is also communicated with the microchannel main path between the sample storage pool and the reaction detection pool by a microchannel connecting branch path. The microfludic immunodetection chip and method disclosed by the invention have the advantages that the sample treating time is greatly shortened, the cost is reduced, the detection accuracy can also be improved and the functions of mobile detection andhousehold self-service type detection and the like are realized.

The invention discloses a site-specific integration method of exogenous genes, and particularly discloses a method for integrating the exogenous genes into second exons of bovine beta-casein genes in a site-specific manner. The method comprises the following steps: expressing TALE (Transcription Activator Like Effector) protein-I and TALE protein-II in isolated bovine cells so as to obtain cells, the target sequences of second exons of which change suddenly; meanwhile, recombining the exogenous genes into the target sequences in a homologous manner so as to realize the site-specific integration of the exogenous genes on the second exons of the bovine beta-casein genes. The method has the advantages that human lysozyme genes or other target genes are capable of transcribing the site-specific integration of very active beta-casein gene loca in mammary gland, the location effect is overcome, the target gene expression silencing is avoided, the relatively high expression level of the human lysozyme or other recombinant protein in mammary gland can be realized, the success rate of transgenic breeding is increased, and meanwhile, the cost of producing and purifying the recombinant protein in a later period can be saved.

The invention relates to the field of molecular biology, in particular to an application of serine protease (PoCFI-Tryp) of a flounder complement regulatory protein I factor. The invention relates tothe application of serine protease (PoCFI-Tryp) in the flounder complement regulatory protein I factor in preparation of a bacteriostatic agent. The serine protease disclosed by the invention can be combined with various bacteria, and can obviously inhibit the growth of Edwardsiella tarda, Vibrio anguillarum, Vibrio harveyi and Streptococcus iniae. The obtained protein has the application potential in bacterial infection resistance.

The invention relates to a method for preparing sesame oil through water-method demulsification of a sesame oil body and belongs to the technical field of processing of vegetable fat and proteins. According to the method, the sesame oil and sesame proteins are prepared through the preparation of sesame slurry, the sesame oil body, sesame oil I and sesame protein I, the separation of the sesame oil body in a heavy phase and the preparation and combination of sesame protein II. The thermal processing is omitted during the preparation of the sesame slurry (or referred as to a sesame water phase extract), so that the influences caused by the thermal processing to the sesame proteins and other components are avoided; by carrying out efficient demulsification on a light phase (the sesame oil body) obtained through centrifugation, the clear and mellow sesame oil can be obtained; and by virtue of condition optimization, more than or equal to 98% of oil in the sesame oil body can be released through centrifugation. The method has the beneficial effects that the process is simple, and main equipment is a centrifugal machine; and no any enzyme preparation is used, and no organic solvent is used, so that the preparation process is environmentally friendly and efficient.

The invention provides a method for recovering protein from potato starch processing wastewater, which comprises the following steps: standing, precipitating and filtering the potato starch processingwastewater to obtain pretreated wastewater; adding alkali into the pretreated wastewater, adjusting the pH of the pretreated wastewater, carrying out ultrasonic treatment to obtain an alkaline solution, and separating the alkaline solution to obtain a potato protein solution; adding acid into the potato protein solution, adjusting the pH value of the potato protein solution, carrying out acid precipitation treatment, separating to obtain potato protein I and acid precipitation supernatant, and filtering the acid precipitation supernatant through an ultrafiltration membrane to obtain potato protein II; and uniformly mixing the potato protein I, the potato protein II and water to obtain potato protein slurry, adjusting the pH value of the potato protein slurry, adding a compound enzyme forenzymolysis to obtain an enzymolysis product, and treating the enzymolysis product to obtain potato protein. The method solves the problem that the recovery rate of protein in the potato starch processing wastewater is low.

The invention provides a group of metastasis suppressor1 (Mtss1) protein dimerization fluorescent probe complementary proteins containing amino acid sequences as shown in SEQ ID No: 2 and SEQ ID No: 4 which respectively correspond to a fluorescence probe N-terminal protein and a fluorescence probe C-terminal protein. The invention also provides polynucleotides encoding the group of proteins and recombinant vectors of the polynucleotides. The fluorescence probes provided by the invention can make use of dimerization of an Mtss1 protein, and end-connecting complementation genes of the fluorescence probes are close to each other and interact with each other to form an ability to excite fluorescence. The fluorescence intensity of the probes can indicate the dimerization extent of an I-BAR district of the Mtss1 protein, and shows a linear relationship with activities of Mtss1 protein I-BAR inhibiting drugs. The probe proteins provided by the invention can be used for studying an Mtss1 protein dimerization process, and can also be used for high efficiency screening of Mtss1 protein inhibitors.

A novel polypeptide-human NADH dehydrogenase substructure protein I-9.25, the polynucleotide for coding it, the process for preparing said polypeptide by DNA recombination, the application of said polypeptide in treating diseases, such as cancer, HIV infection, immunopathy, etc. the antagon of said polypeptide and its medical action, and the application of said polynucleotide are disclosed.

The invention discloses application of human serum pancreatic regenerated protein I alpha (REGI alpha) as a detection target or a standard substance in preparation of a reagent or a kit for predictingtumors, and finds that the tumors can be accurately predicted according to the level of the REGI alpha protein in human serum. The brand-new marker human serum REGI alpha protein for predicting tumors provided by the invention can be used as a detection target for predicting or assisting in judging the occurrence and development of tumors of patients. Clinical research results show that serum REGI alpha detection has an area under a tumor diagnosis ROC curve of 0.764, sensitivity of 62.5% and specificity of 87.5%. The optimal cutoff value (cutoff) for diagnosing tumor resistance is 46.97 ng / ml.

The invention relates to substituted aryl and heteroaryl (R)-Chiral Halogenated 1-Substitutedamino-(n+1)-Alkanol compounds useful as inhibitors of cholesteryl ester transfer protein (CETP; plasma lipid transfer protein-I) and compounds, compositions and methods for treating atherosclerosis and other coronary artery diseases. Novel high yield, stereoselective processes for the preparation of the chiral substituted alkanol compounds from chiral and achiral intermediates are described.

The present invention relates to a recombinant antibody against human cardiac troponin I, in particular to a novel isolated binding protein containing cTnI antigen binding domain, and researches on the preparation and application of the binding protein. The binding protein has strong activity and high affinity with human cTnI protein, and can be widely used in the detection field of cTnI protein.

The invention discloses a microfludic immunodetection chip and method of cardiac troponin protein I. In the microfludic immunodetection chip, a sample storage pool, a reaction detection pool and a waste-liquid pool are arranged on a microchannel main path in sequence; one end of the microchannel main path is provided with a sampling port, and the other end of the microchannel main path is connected with a first pump; one end of a microchannel branch path is communicated with a water storage pool by a second pump, and the other end of the microchannel branch path is connected with the microchannel main path between the reaction detection pool and the waste-liquid pool; the microchannel branch path is provided with a reagent B filling pool and a high-value calibration pool in sequence; a low-value calibration pool is connected with the high-value calibration pool in parallel; the microchannel branch path between the reagent B filling pool and the high-value calibration pool is also communicated with the microchannel main path between the sample storage pool and the reaction detection pool by a microchannel connecting branch path. The microfludic immunodetection chip and method disclosed by the invention have the advantages that the sample treating time is greatly shortened, the cost is reduced, the detection accuracy can also be improved and the functions of mobile detection andhousehold self-service type detection and the like are realized.

The invention discloses an IL-2 receptor compound as well as a preparation method and application thereof. The IL-2 receptor compound is obtained by constructing nucleic acid sequences for encoding afusion protein I (SEQ ID NO: 1 or 7) and a fusion protein II (SEQ ID NO: 3 or 9) to the same eukaryotic expression vector or respectively constructing the nucleic acid sequences to different eukaryotic expression vectors, transfecting mammalian cells by using the obtained recombinant expression vectors, and separating and purifying from cell culture supernatant. An IL-2 receptor alpha beta gamma heterotrimer protein is directly obtained through recombinant expression and one-step affinity chromatography purification by utilizing a genetic engineering technology, and in-vitro assembly is not needed. The heterotrimer protein has high affinity activity with IL-2, and can be used for in-vitro research of interaction between IL-2 and IL-2 receptors, preparation of antibodies from immune animals, in-vitro screening of antibodies and the like.

The invention discloses application of a protein ATCTPA2 related to photosynthesis, and provides an application of the coding gene of the protein ATCTPA2 to protecting the photosynthesis of a plant photosystem II, wherein the protein ATCTPA2 is a protein I which is composed of an amino acid sequence shown in a sequence 2 in the sequence table, or a protein II which is obtained by replacing the amino acid residue sequence in the sequence 2 in the sequence table with one or more amino acid residues, and / or eliminating and / or adding one or more amino acid residues, is provided with the same activity as the protein I, and is derived by the protein I. The experiment of the invention shows that the protein ATCTPA2 which is related to photosynthesis and is discovered from arabidopsis can recuperate etiolated seedling of an arabidopsis mutant with reduced ATCTPA2 expression into green seedling.

The present invention relates to a polypeptide derivative that promotes cell proliferation, a preparation method and application thereof. The polypeptide derivative has the following structure: Nap-FFGGYGSSSRRAPQT. The present invention also provides a preparation method of the above-mentioned polypeptide derivative. The polypeptide derivative adopts FMOC-solid phase synthesis method. The present invention also provides the use of the above-mentioned polypeptide derivatives in medicines for promoting cell proliferation. The invention is simple to synthesize, and can synthesize active self-assembled polypeptides by solid-phase synthesis, and the raw materials used are amino acids necessary for the human body every day; secondly, because of its self-assembly, it can be slowly released in the body, which significantly solves the problem of IGF- After injection, protein I is easily degraded by various proteases in the body, which leads to the problem of low retention rate in the body; most importantly, it can bind to the IGF-I protein receptor, which in turn can stimulate downstream signaling pathways, promote endothelial cell proliferation, and promote Vascular repair and regeneration.

The invention discloses application of a protein ATCTPA2 related to photosynthesis, and provides an application of the coding gene of the protein ATCTPA2 to protecting the photosynthesis of a plant photosystem II, wherein the protein ATCTPA2 is a protein I which is composed of an amino acid sequence shown in a sequence 2 in the sequence table, or a protein II which is obtained by replacing the amino acid residue sequence in the sequence 2 in the sequence table with one or more amino acid residues, and / or eliminating and / or adding one or more amino acid residues, is provided with the same activity as the protein I, and is derived by the protein I. The experiment of the invention shows that the protein ATCTPA2 which is related to photosynthesis and is discovered from arabidopsis can recuperate etiolated seedling of an arabidopsis mutant with reduced ATCTPA2 expression into green seedling.

The invention provides a method for opening the V domain of natural β2 glycoprotein I, an antigen prepared by the method, an application and a kit, and relates to the technical field of antigen preparation. The method for opening the V structural domain of natural β2 glycoprotein I of the present invention comprises the following steps: the natural β2 glycoprotein I is dialyzed for the first time, then mixed with bacterial lipid A, and reacted to obtain the β2 sugar opened by the V structural domain Protein I; the dialysate used in the first dialysis includes buffer A; the buffer A contains sodium ions at a concentration of 0.75-2M, and the pH is 9-12. Compared with the natural β2 glycoprotein I antigen and the recombinant β2 glycoprotein I antigen, the sensitivity of the β2 glycoprotein I with the V domain opened prepared by the method is greatly improved.

The invention discloses application of snow chrysanthemum extract and snow chrysanthemum total flavone in preparation of a drug for treating hepatic fibrosis. According to a cell experiment and an animal experiment, the snow chrysanthemum extract is given to a hepatic stellate cell line LX-2 and a CC14-induced hepatic fibrosis rat model separately, and a cell experiment result shows that the snow chrysanthemum extract can significantly reduce the DNA binding capacity of NF-kappa B, lower fibrosis markers alpha smooth muscle actin and cell matrix protein I type collagen and promote LX-2 cell apoptosis; an in-vivo result shows that compared with the model group, the snow chrysanthemum extract can decrease the content of AST and ALT of a hepatic fibrosis rat, decrease the content of hepatic collagen and reduce expression of the alpha-SMA and an ECM, and after hepatic tissue is subjected to ultrasonication to form homogenate suspension, it can be found that the DNA binding capacity of the NF-kappa B is significantly reduced; the snow chrysanthemum extract can be used for preparing the drug or functional food for treating the hepatic fibrosis by serving as an NF-kappa B signal channel inhibitor.

The invention discloses a method for preparing walnut oil by performing emulsion breaking on a walnut oil body by a water method, and belongs to the technical field of processing of plant oil and proteins. The walnut oil and walnut proteins are prepared by preparing walnut pulp, the walnut oil body, walnut oil I, walnut protein I, the walnut oil body in a heavy phase and walnut protein II, and combining the prepared materials. According to the method, no thermal treatment is carried out in the process of preparing the walnut pulp (or an extract of a walnut water phase), so that the walnut proteins and other components cannot be affected by thermal treatment; a light phase (the walnut oil body) part obtained by centrifugation can be subjected to efficient emulsion breaking by using the method, so as to obtain clear and fragrant walnut oil; by condition optimization, 98 percent or above of oil, obtained by centrifugation, in the walnut oil body can be released. The method is simple in process, and a centrifugation machine is used as main equipment; no enzyme preparation or organic solvent is used, so that the preparation process is environment-friendly and efficient.

The present invention provides a method of treating diabetes, including administering to a mammal diagnosed with diabetes a purified recombinant reg I protein.

The invention provides a method for recovering protein from potato starch processing wastewater, comprising: subjecting the potato starch processing wastewater to static precipitation and filtering to obtain pretreated wastewater; adding alkali to the pretreated wastewater to adjust the pH of the pretreated wastewater And carry out ultrasonic treatment, obtain alkali solution, separate alkali solution, obtain potato protein solution; Add acid to potato protein solution, adjust the pH of potato protein solution and carry out acid precipitation treatment, through separation, obtain potato protein I and acid precipitation supernatant The acid precipitation supernatant was filtered through an ultrafiltration membrane to obtain potato protein II; the potato protein I, potato protein II and water were mixed to obtain a potato protein slurry, and the pH of the potato protein slurry was adjusted and compound enzymes were added for enzymatic hydrolysis Treating to obtain an enzymatic hydrolysis product, and treating the enzymatic hydrolysis product to obtain potato protein. The method solves the problem of low protein recovery rate in potato starch processing wastewater.

The invention discloses a semi-quantitative test paper for detecting an acetylated PINK1 protein in a vascular endothelial cell. The semi-quantitative test paper is mainly composed of five parts including a sample pad, a conjugate pad, a water absorption pad, a bottom plate and nitrocellulose membrane; two parallel antibody capturing lines are respectively drawn on the nitrocellulose membrane, theantibody capturing line close to the conjugate pad contains a concentration-determined acetylated PINK1 protein I monoclonal antibody in a first anti-vascular endothelial cell and is used as a detection line, the other antibody capturing line contains a concentration-determined goat anti-rabbit IgG antibody and is used as a standard concentration color control line, and the distance between the two lines is 4-7 mm; and the conjugate pad is sprayed with two substances including a colloidal gold or colored latex labeled acetylated PINK1 protein I monoclonal antibody in a second anti-vascular endothelial cell and a gold-labeled rabbit IgG.

The invention discloses application of an anti-inflammatory active ingredient of blumea balsamifera in medicines, relates to medicines, and provides influence of the anti-inflammatory active ingredient of blumea balsamifera on expression and phosphorylation level of nuclear transcription factor NF-kB related proteins I kB alpha and p65 and on cell nucleus displacement. The anti-inflammatory activity of the blumea balsamifera is confirmed, the dosage of the blumea balsamifera applied to the medicine is determined to be 5-80 mu g / ml, and a foundation is laid for the application of the anti-inflammatory active component in the medicine.

The invention relates to the field of molecular biology, in particular to the application of a serine protease (PoCFI-Tryp) of a flounder complement regulatory protein I factor. The application of serine protease (PoCFI‑Tryp) in the complement regulatory protein I factor of flounder in the preparation of bacteriostatic agents. The serine protease of the present invention can be combined with various bacteria, and can significantly inhibit the growth of Edwardsiella lentus, Vibrio eel, Vibrio harvey and Streptococcus iniae. The obtained protein has application potential in anti-bacterial infection.