41 results about "Single locus" patented technology

The invention discloses a novel genotype analysis method based on multimodal brain images. With utilization of information, including a structural image and a functional image, of two kinds of modes, regression on the rs429358 locus genotype linked to the Alzheimer's disease closely is realized. The method comprises: extracting voxel features from a brain structure images and extracting connecting network features from a brain function image; carrying out feature extraction on the two kinds of features by using an elastic network and keeping brain area and network connection with a high association degree; and then carrying out fusion and genotype regression on selected nodes and side attributes by using a multi-kernel support vector. The method has the following advantages: for the brain image with the complicated structure and functions and genotype data, a novel regression analysis method for a single locus genotype based on structural and functional multimodal brain images is put forward; the analysis effect is good; and complementary information of the two modes is utilized fully.

The invention is directed to methods for highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune heterodimers from a large number of biological samples containing lymphocytes of interest. Methods of the invention comprise performing a single pairing assay on a pool of source samples to determine nucleic acids encoding paired cognate receptor heterodimer chains in the combined pool. Separately, single-locus, high-throughput sequencing is performed on each individual sample to determine the plurality of sequences encoding one of the two receptor polypeptide chains. Pairs of cognate sequences determined in the pooled sample may then be mapped back to a single source sample by comparing the expression patterns of the single-locus sequences.

The invention discloses a kit for detecting folic acid utilization ability gene and a detection method of the kit, relating to the technical field of gene detection, wherein the kit for detecting folic acid utilization ability gene comprises (1) a PCR (polymerase chain reaction) reaction reagent including dNTP, 5* and 10*PCR buffer liquid, Mg<2+> ion, de-ionized water, FastTaq enzyme, SNaPshot Mix mixed liquid; (2), a mixture obtained by mixing 16 pairs of PCR primers according to a certain proportion; (3), a mixture obtained by mixing 16 extended primers according to a certain proportion; (4), SAP enzyme for purification, Exon I enzyme and buffer liquid matched with the enzymes; (5), positive and negative control DNA with single locus subjected to homozygous or heterozygous mutation; (6), an instruction manual. The method provided by the invention is simple, high in flux, high in effect and low in cost, and is suitable for detecting folic acid utilization ability gene for Chinese population.

A method of determining the presence of a novel structural variation in a genome using long-read genome sequence fragments includes a process of aligning, filtering ranking and linking long-read sequence fragments against a reference genome. Presence of a novel structural variation is present in said genome can be determined when said linked alignment contains multiple linked fragments that are mapped to a single locus, referred to as a split-read.

The invention provides a verifying method of a high-throughput sequencing mutation detection result and a method for mutation searching through the high-throughput sequencing result. According to the verifying method, mathematical calculation and statistical formulas are combined, whether interested single-locus special base substitution exists in a high-throughput sequencing sample or not is determined by calculating the mutation frequency of the interested single-locus special base substitution, and whether interested continuous-locus base deletion or interested continuous-locus special base substitution exists or not is determined by calculating the variation coefficient of the interested continuous-locus base deletion or the interested continuous-locus special base substitution.

The invention provides an identification method of pig fat deposition related PNPLA2 mRNA m6A methylation single locus. According to m6A-seq sequencing, m6A methylation locus exists in a fragment of about 100nt, and method is characterized in that confirmation of a m6A single locus position of a PNPLA2 gene is carried out according to a RRACH(R=G,A;H=A,C,T) sequence with highly conserved methylation. Bioinformatics analysis is used, and according to a conserved sequence of m6A, the concrete locus of m6A is identified; the invention provides a primer sequence and a concrete method for identification of methylation level of PNPLA2 mRNA m6A in pigs; gene single locus mutation changes PNPLA2 m6A level proves that the PNPLA2 m6A locus has function for influencing fat deposition.

Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

The invention relates to a pair of single-locus specific primers for amplifying class II major histocompatibility complex genes for antibacterial potential detection of the alligator sinensis and a typing method, wherein the specific sequences of the primers are as shown in SEQ ID NO. 1-SEQ ID NO. 2. The amplification primers disclosed by the invention are stable in single-locus specific amplification capacity in an alligator sinensis population; a product obtained from amplification can conduct genotyping on various individuals through a subsequent single strand conformation polymorphism (SSCP) experiment, so that the polymorphism of the class II MHC genes of alligator sinensis individuals in the population is assessed and the potential of the alligator sinensis individuals in resisting bacterial diseases is detected; therefore, an important reference is provided for such operations of new population founder selection, reintroduction individual selection, artificial mating selection and the like in alligator sinensis protection work.

The invention discloses a poppy species specificity genetic marker detecting system. A primer pair set for identifying poppy is provided and is composed of a primer pair P12 (sequence 1 and 2), a primer pair P13 (sequence 3 and 4), a primer pair P5 (sequence 5 and 6) and a primer pair P14 (sequence 7 and 8). Four STR loci of poppy are amplified at the same time, and the amplification is more accurate and rapid compared with amplification of a single locus; the system has higher species specificity when identifying poppy and affinis plants, and an effective method is provided for further studying poppy STR loci and inspecting and identifying the poppy species in a case; in an optimized poppy STR composite amplification system, amplification of all loci can be balanced, sensitivity is high, stability is high, and frequently-seen corrosion check materials in a drug-related case can be easily inspected; accuracy is high, parting of four loci of each of poppy samples from different producing areas can be achieved through detection and data statistic analysis.

The invention discloses a method for detecting a chicken tenderness character by using single nucleotide polymorphism (SNP) and primers. The method comprises the following steps: carrying out SNPs detection on CDS (Coding Sequence) regions of CAPN1 (Calcium-Activated Neutral Protease) and CAST (Calpastatin) genes of a chicken, and detecting one SNP locus on an exon 6 of the CAPN1 gene by using a primer F1, wherein the SNP locus is concretely the 3535th position G->A on a GenBank sequence (GenBank NO:NC-006090.1); detecting one SNP locus on an exon 11 of the CAST gene by using a primer F2, wherein the SNP locus is concretely the 37752nd position A->T on a GenBank sequence (GenBank NO:NC-006127.2); and analyzing the relationship between different gene types of a single locus and the tenderness character in a high-quality broiler chicken.

The present disclosure describes a method for identifying a strain or species of bacteria using a single locus sequence typing technique. The single locus useful in the method is the promoter region of the 16S rRNA operon. The method is useful to identify infectious bacteria in a subject, to identifying contaminants in a food source, as well as strain typing and genetic fingerprinting of bacterial families.

The invention provides a human tumor necrosis factor-alpha (hTNF-alpha) mutant protein, and discloses the characteristic of generating or enhancing the immune response aiming at TNF-alpha molecules in an organism. With a genetic code expansion technology, a non-natural amino acid p-nitrophenylalanine (pNO2Phe) is introduced to the 11 and/or 87 locus in hTNF-alpha, such that hTNF-alpha mutant protein is obtained. Through immunizing animals, the mutant protein can stimulate an organism to produce cross antibodies to react with the disease-related natural inflammatory factor TNF-alpha, such that the immune tolerance of hTNF-alpha as an autologous protein vaccine is broken through. Compared with prototype hTNF-alpha and a single-locus mutant, the immunogenicity of the two-locus mutant protein is enhanced. The mutant protein can be used as a candidate molecule for treating autoimmune diseases.

The invention discloses a fusion protein based on CjCas9 and VPR core structure domains, a corresponding DNA targeting activation system and application of the system. The fusion protein comprises twoheterologous polypeptide structure domains, and one polypeptide structure domain comprises VPR protein with the transcription activation activity; the other polypeptide structure domain comprises theCjCas9 protein, the CjCas9 protein is in a dCjCas9 subtype or a mini-dCjCas9 subtype or a CjCas9 wild type, and the dCjCas9 subtype contains D8A and H559A single-locus amino acid mutation; the mini-dCjCas9 subtype contains D8A single-locus amino acid mutation and deficiency of most HNH structure domains. The DNA targeting activation system comprises the fusion protein and one or more kinds of guide RNA, and the guide RNA comprises a sequence with the length of 14-22 bp and a framework sequence with the length of 80 bp, wherein the sequences are designed for a target gene promoter area. The DNA targeting activation system is applied to targeting activation of a gene. The DNA targeting activation system has the advantages of being small in size, capable of achieving high specificity, a highactivation effect and easy synthesis, low in cost and the like.

The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; amplifiable selection marker; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all integrated at a single locus within the retroviral producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof. The nucleic acid vector additionally comprises nucleic acid sequences encoding an amplifiable selection marker. The invention also relates to uses and methods using said nucleic acid vector in order to produce stable retroviral packaging and producer cell lines.