The invention discloses a
genome assembly method for constructing an overlength continuous
DNA (DeoxyriboNucleic Acid) sequence. The
genome assembly method comprises the following steps: S1, finding out an overlapping region between each pair of known
DNA sequences; S2, starting from a free
tail end of any anchoring sequence fragment, extending the anchoring sequence fragment by using a Read sequence overlapped with the anchoring sequence fragment,
cycling for multiple times until the Read sequence which can match the
tail end of the other anchoring sequence fragment is encountered and obtaining one or more path sequences; S3, selecting at most one sequence from all the path sequences as an effective
linker sequence for linking the
tail end of a starting anchoring sequence fragment to thetail end of another endpoint anchoring sequence fragment; S4, linking the starting anchoring sequence fragment with the corresponding endpoint anchoring sequence fragment by utilizing the effective
linker sequence; taking the effective
linker sequence as a new anchoring sequence fragment after linking or recording the free tail end of the remaining anchoring sequence fragment and switching to S2;repeating S2 to S4 and finally, forming the overlength continuous
DNA sequence. The
genome assembly method disclosed by the invention has the
advantage that the sequences of the
whole chromosome and the whole genome are more favorably restored.