The invention discloses an optimization method for efficiently and quickly building an ophiopogon japonicus saturation high resistance homogeneous mutant library. Ethylmethane sulfonate (EMS) and <60> Co-gamma ray are physical and chemical mutagenic agents which are most widely used, efficient and stable. The EMS can generate a plurality of point mutations on one genome, the generated mutations can be randomly distributed on the whole genome, the distribution is even, the density is high, the cost is low, and the obtaining of allelic mutants is facilitated, especially, a saturated mutant library can be obtained by a smaller mutant population; Gamma ray mutagenesis mainly causes the loss of DNA fragments and chromosomal rearrangement and is specially suitable for the building of a mutant library of which multiple family gene functions are lost; therefore, by using the two method simultaneously to make their respective advantages complementary to each other, the capacity of the mutant library is increased, the demands of the saturated mutant library are satisfied, the content of the saturated mutant library is enriched, and moreover, the obtained mutants do not involve a GM event, so the safety is high and the application is convenient.