Presence of erg gene rearrangements and protein over-expression in low grade PIN (LG-PIN) in prostate biopsies

A technology of LG-PIN and prostate, which is applied in the direction of drug combination, biochemical equipment and methods, and microbial measurement/inspection, and can solve problems such as lack

Active Publication Date: 2013-11-13
VENTANA MEDICAL SYST INC
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Problems solved by technology

Basal cell-specific immunostaining for cytokeratins is present in the PIN but absent in the PC region

Method used

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  • Presence of erg gene rearrangements and protein over-expression in low grade PIN (LG-PIN) in prostate biopsies
  • Presence of erg gene rearrangements and protein over-expression in low grade PIN (LG-PIN) in prostate biopsies
  • Presence of erg gene rearrangements and protein over-expression in low grade PIN (LG-PIN) in prostate biopsies

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Embodiment

[0047] The aim of this study was to identify the extent of ERG rearrangements in PIN lesions of the prostate. The inventors developed an automated staining protocol to detect ERG overexpression by immunohistochemistry (IHC) in combination with detection of specific gene rearrangements by FISH assay. To this end, probes specific for ERG gene rearrangements comprising 3' and 5' ERGs were detected with quantum dot (Life Technologies, OR) bioconjugates. Fixatives commonly used for needle biopsy preservation and the duration of fixation were also assessed. Specific FISH signals were deconvoluted using an interferometer and spectral imaging software.

[0048] Specific methods for spectroscopic FISH with quantum dots in formalin-fixed and paraffin-embedded tissues are described in the literature. Briefly, spectral image cubes containing high-resolution wavelength intensity information at each pixel were captured using a modified SpectraView (ASI; Applied Spectral Imaging, Israel) a...

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Abstract

The present disclosure relates to methods for the early detection of ERG over-expression, gene rearrangements, and diagnosis and / or prognosis of prostate cancer using a combined ERG IHC-FISH assay.

Description

[0001] related application [0002] This application is filed claiming the benefit of US Provisional Patent Application No. 61 / 446,300, filed February 24, 2011. field of invention [0003] The present disclosure relates to methods for early stage detection of previously detectable prostate cancer cells in biological samples as cancer pathology. More specifically, the present disclosure relates to a combined anti-ERG immunohistochemistry assay coupled to an ERG FISH assay that results in the identification of low-grade PINs that are more likely to be associated with, and thus predictive of, cancer. Background of the invention [0004] Early detection of cancer is a key feature in treating cancer patients. In men, prostate cancer is the most prevalent form of cancer of all races. Although more than 300,000 men are diagnosed with prostate cancer each year in the United States alone, currently available tests are notoriously inaccurate and subjective. Consequently, many cases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6841C12Q2600/156C12Q1/6886G01N2333/4703G01N33/57434A61P35/00
Inventor G.佩斯塔诺R.内格尔U.萨斯亚纳拉亚娜A.D.纳吉C.科尔特斯K.加沙R.迪塔莫尔
Owner VENTANA MEDICAL SYST INC
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