78 results about "Chromatosome" patented technology

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention provides an adaptive weight distribution chromosome stripe contrast enhancement method and system, and the method comprises the steps: firstly improving the significance of a chromosome stripe structure and an edge contour in a chromosome gray image through an edge enhancement convolution operator; carrying out chromosome monomer target detection and instance segmentation on the metaphase chromosome based on an instance segmentation network of deep learning to obtain a chromosome monomer ROI region; then, extracting a structure tensor response of the ROI region of the chromosome monomer, designing an adaptive enhancement weight of a histogram merging interval based on a normalized difference between a response value and global statistical information, and performing reconstruction and interpolation on a histogram of the image of the ROI region of the chromosome monomer; and finally, adaptively enhancing the contrast of the chromosome stripe through iterative histogram matching. According to the method, the original image texture structure is kept, and meanwhile, the definition and the distinguishability of chromosome stripes are effectively and adaptively improved.

The invention relates to a biological information data processing technology, in particular to a method for analyzing a single-cell Hi-C regulation scale chromatin band, which comprises the following steps: preprocessing single-cell Hi-C data to generate pseudo-batch Hi-C data; performing normalization processing on the pseudo batch Hi-C data to extract a Hi-C contact matrix of each chromosome; identifying and detecting false batch strips from the Hi-C contact matrix; projecting the pseudo batch strips to the original single cell Hi-C data to obtain single cell strips; and carrying out quantitative analysis on the single-cell strip in the original single-cell Hi-C data. According to the method, the regulation and control scale chromatin bands with definite endpoints and directivity can be stably identified, and a band set with remarkable statistics is output.

Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1 / 2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

A method for detecting structural variations in chromosomes by labeling single-stranded chromatids with different colored probes is disclosed. The hybridization patterns of the labeled probes generate spectral profiles that enable high-resolution detection of structural variations, facilitating the distinction between benign and deleterious structural variations. Furthermore, the spectral profiles provide information about complex structural variations that may result from more than one rearrangement of chromosomal segments. The spectral profiles can be used to generate data tables, which can then be subjected to node analysis to identify structural features of interest.

It was discovered that by binding to CTCF which is important for maintaining chromosomal structures, hSATII RNA inhibits the function thereof, changes chromosome interaction, and induces transcription of inflammation-associated genes. It has also been discovered that cell death can be selectively induced in senescent cells and cancer cells by suppressing hSATII RNA expression. From the obtained results, it has been found that cancer treatment which targets senescent stromal cells and cancer cells can be performed with a substance that suppresses hSATII RNA or a substance that increases CTCF expression. In addition, cancer can be detected early by measuring the expression and activity of hSATII RNA and CTCF, and an epigenomic change of the hSATII DNA region. Thus, it is possible to provide a therapeutic drug for a cancer associated with cellular senescence, a method for screening thereof, and a test support method.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Described herein are methods for making genetically modified cells by introducing combinations of genetic variants (designed or random) or constructs (genes or otherwise arbitrary DNA) into a population of cells, and for tracking each variant combination by sequentially building an array of barcodes at a common locus (chromosomal or plasmid), termed the barcode locus. Also described are the cells made by such methods.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

This invention relates generally to the discovery of an improved method to differentiate histiocytic malignancy from lymphoma or hemangiosarcoma in dogs.

Provided herein are achromosomal dynamic active systems comprising a Type 1 pilus (TIP) and methods of making and using the same.

The present application relates to biological information data processing technology, and is a method for single-cell Hi-C regulatory scale chromatin band analysis, comprising the following steps: preprocessing single-cell Hi-C data to generate pseudo-bulk Hi-C data; normalizing the pseudo-bulk Hi-C data to extract the Hi-C contact matrix of each chromosome; identifying and detecting pseudo-bulk bands from the Hi-C contact matrix; projecting the pseudo-bulk bands onto the original single-cell Hi-C data to obtain single-cell bands; and quantitatively analyzing the single-cell bands in the original single-cell Hi-C data. The present application can stably identify regulatory scale chromatin bands with clear endpoints and directionality, and output statistically significant band sets.

The present disclosure provides novel compositions and methods suitable for specifically eliminating target cells (e.g., cancer cells) without affecting non-target cells (e.g., non-cancer cells). For example, CRISPR system and the compositions of the present disclosure can be employed to specifically introduce a suicidal gene into a cancer cell in the loci of a cancer-specific target sequence, which as a result of chromosomal re-arrangement or translocation in a cancer cell presents a cancer specific sequence for a guide RNA and CAS to be recognized and such sequence is absent in a non-cancer cell. Consequently, the specific introduction of the composition(s) to cancer-specific site(s) and integration of suicide gene in the target genome, which is inapplicable to normal cells for lack of the site(s), leads to selective elimination of cancer cells but not non-cancer cells, and therefore render novel therapeutic methods and compositions for cancer treatment.

The application discloses a Pichia pastoris engineering bacterium for converting methanol to synthesize bakuchiol from scratch as well as construction and application of the bacterium. In different chromosomal neutral sites of the Pichia pastoris, a heterologous p-coumaric acid synthesis pathway is introduced, key genes of tyrosine and phenylalanine synthesis pathways of a heterologous shikimic acid pathway are knocked out, overexpressed or heterologously expressed, a high-yield p-coumaric acid strain is obtained, on the basis of the strain, a heterologous bakuchiol biosynthesis pathway is introduced, bakuchiol synthetase is overexpressed, key genes of a MVA pathway and genes of an exogenous acetyl coenzyme A supply pathway are endogenously overexpressed or heterologously expressed, after optimization of a methanol concentration and back complementation of a His4 gene of the strain, the yield of the bakuchiol is effectively increased to 91.2 mg / L, which is 59.8 times higher than that of an initial strain, and the yield of the bakuchiol in a 15L fermenter reaches 692.8 mg / L. The application has the characteristics of high conversion efficiency, low production cost, easy preparation, wide industrial application prospect and the like.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

A transgenic Cory fish comprising a chromosomally integrated expression cassette encoding an orange fluorescent protein (OFP). The Cory comprises an orange Cory 1 transformation event, cryopreserved a

Methods of transferring large sequence fragments between chromosomes and generating chromosomal rearrangements using double strand break repair pathways and homology directed repair. Further relates to chromosomes produced by these methods, and cells and transgenic animals comprising these chromosomes.

The present invention relates to a method for detecting chromosomal structural variations using chromosome conformation capture technology and high-throughput sequencing technology, as well as detection products for use in the method, and diagnostic applications of the method and products, in particular in prenatal screening.

The invention discloses a method for improving collagen yield of mesenchymal stem cells and engineered mesenchymal stem cells for high-yield collagen. The method comprises the following steps: designing at least one guide RNA (Ribonucleic Acid), and gRNA targets a promoter region of a collagen gene; the method comprises the following steps: introducing a composition containing gRNA and an expression vector for coding a CRISPR activation system into MSCs; forming a compound in the MSCs, specifically binding the compound to a collagen gene promoter region, and activating the transcription of the collagen gene; engineering MSCs with the expression quantity higher than that of untreated MSCs are screened and separated. The content of collagen produced by the method disclosed by the invention is obviously increased. Meanwhile, the effect is stable, the cell strain is not easy to lose along with cell division, and the yield of the screened cell strain is highly uniform and stable. More importantly, in the aspect of safety, the core CRISPRa method does not cut DNA, so that off-target damage and chromosome abnormality risks caused by DNA breakage are avoided, the potential risk is smaller, and it is ensured that the improved collagen really has biological functionality.

The invention relates to a group of Y-chromosomal short tandem repeat (Y-STR) markers comprising at least one rapidly mutating (RM) Y-STR marker selected from the group consisting of DYF1000, DYF1001, DYF1002, DYR88, DYS685, DYS688, DYS712, DYS1003, DYS1007, DYS1010 and DYS1012. The invention further relates to a set of amplification primers comprising primers for the amplification of at least one Y-STR marker according to the invention, to methods for amplifying an allele of at least one Y-STR marker, to a kit for identifying an allele of a Y-STR marker by amplification and electrophoretic detection or sequencing detection, and by sequencing of non-amplified DNA, and to the use of the group of Y-STR markers for typing male individuals.

Disclosed herein are systems and methods for performing secondary analysis of nucleotide sequencing data in a time-efficient manner. Some embodiments include iteratively performing secondary analysis as sequence reads are generated by a sequencing system. Secondary analysis can include alignment of sequence reads to a reference sequence (e.g., a human reference genome sequence) and use of the alignment to detect differences between a sample and the reference. Secondary analysis can be capable of detecting genetic differences, variant calling and genotyping, identifying single nucleotide polymorphisms (SNPs), small insertions and deletions (indels), and structural changes in DNA, such as copy number variations (CNVs) and chromosomal rearrangements.

The present disclosure relates generally to compounds that inhibit PRMT5. The disclosure further relates to the use of these compounds for the preparation of a medicament for the treatment of diseases and / or conditions by inhibition of PRMT5. The disclosure further relates to the use of these compounds for the treatment of diseases or conditions associated with chromosomal 9p21 deletion, MTAP invalidation, or any other MTAP deficiency. The disclosure further relates to the use of these compounds for the treatment of cancer.

Disclosed herein is a non-chromosomal dynamic activation system (ADAS) derived from a parent bacterial cell, the ADAS comprising at least one genetic loss-of-function modification in a lytic enzyme to increase the stability of the ADAS. Also disclosed is a method for disrupting sporulation in the parent bacterial cell in combination with a lytic enzyme deletion or other loss-of-function mutation.

The application discloses a SNP molecular marker related to intramuscular fat content of Laiwu pigs and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker corresponds to a SNP site located at 27,674,494 bp of chromosome 10 in the 11.1 version of an international pig reference genome, and is located in a chromatin open region of a CTSL gene; the chromatin open region comprises a positive strand sequence as shown in SEQ ID NO. 1 and an antisense strand sequence as shown in SEQ ID NO. 2; a T / C polymorphism exists at the 206th base of the SEQ ID NO. 1, and an A / G polymorphism exists at the 377th base of the SEQ ID NO. 2. By detecting the SNP molecular marker, early judgment of intramuscular fat content can be realized at a piglet stage of the Laiwu pigs, a breeding cycle is greatly shortened, breeding cost is reduced, and the stability of the high intramuscular fat content trait of the Laiwu pigs is effectively maintained, so that the application has important application value for Laiwu pig breeding.

This invention provides molecular markers, typing primers and their applications for the restoration of cytoplasmic male sterility in chili pepper. Wild-type and mutant chili pepper materials were used to construct an F2 population, and the chromosomal region closely linked to the cytoplasmic male sterility restoration gene in chili pepper was obtained using the BSA population positioning method. A KASP molecular marker was designed based on a single base mutation, and this marker was used to identify the genotypes of 1290 plants in the F2 population, with a match rate of 100%. This invention is not only useful for the selection and assisted breeding of chili pepper for cytoplasmic male sterility, but also provides a basis for the mapping of the cytoplasmic male sterility restoration gene and the analysis of the molecular mechanism of cytoplasmic male sterility, and has widespread value.

The invention discloses an eQTL (quantitative trait loci) molecular marker related to the hypoxia resistance character of trachinotus ovatus and application of the eQTL molecular marker. The SNP molecular marker obviously related to the low-oxygen tolerance character of the trachinotus ovatus is obtained by integrating whole genome re-sequencing, transcriptome sequencing and chromatin open omics analysis, the eQTL molecular marker is positioned in a promoter region of 1711-1881bp upstream of a pla2g1b gene of the trachinotus ovatus, the genome position of the eQTL molecular marker is 16650700-16650870 bp of a chromosome 20, and the corresponding sequence is SEQ ID NO. 1. The eQTL molecular marker comprises four SNP (Single Nucleotide Polymorphism) sites, and the genomic positions of the four SNP sites are respectively 16650770 bp, 16650804 bp, 16650828 bp and 16650849 bp of a chromosome 20 of the four SNP sites. The eQTL molecular marker can be used as an auxiliary selection marker in trachinotus ovatus hypoxia adaptability molecular breeding, the screening efficiency of hypoxia tolerance strains can be effectively improved, and the breeding process of new varieties is accelerated.

Provided is a method for detecting early ovarian cancer (EOC) for a patient, comprising: obtaining one or more embedding representations for each of a plurality of chromosomes of the patient; inputting one or more embedding representations into a machine-learning model, wherein the machine-learning model is trained by: obtaining DNA methylation data of a plurality of subjects; pretraining, based on the DNA methylation data of the plurality of subjects, the machine-learning model to predict a methylation level of a masked CpG site; and training the pretrained model to detect EOC; and detecting EOC for the patient based on an output of the machine-learning model. Provided is transformer-based AI for early cancer diagnosis using cfDNA methylation markers, such as a transformer-based AI technology that improves early ovarian cancer diagnosis using cfDNA methylation markers.