121 results about "Chromatosome" patented technology

The invention relates to the technical field of biology, in particular to a molecular marker for accurately positioning centromere of chromosome of Chinese trumpet creeper, and the sequence of the molecular marker is as shown in SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: specifically enriching a DNA (Deoxyribose Nucleic Acid) sequence combined with a centromere specific histone CENH3 through a chromatin co-immunoprecipitation technology (ChIP), and sequencing the DNA sequence; sequencing data are analyzed to obtain a centromere sequence of the vicia villosa, and two specific repetitive sequences of the centromere of the vicia villosa are obtained after identification and optimization. Primers are designed according to the sequences, PCR amplification is carried out, amplification product sequences are marked, fluorescence in situ hybridization verification is carried out by analyzing a cytogenetics technology, it is proved that the primers are specifically distributed in a centromere area of vicia villosa, and the centromere specificity of the primers is proved. The marker can be used for genetic identification and accurate positioning of the centromere in genome research, and can also be used for cytological identification of the centromere and karyotype research such as chromosome counting and morphological analysis through centromere signal observation.

The present disclosure provides a Chinese Hamster Ovary (CHO) cell comprising one or more exogenous nucleotide sequences, wherein the one or more exogenous nucleotide sequences is inserted into a chromosomal locus of the CHO cell, wherein the chromosomal locus is located within contig NW_023276805.1 of chromosome X. Also provided herein are methods of producing a CHO cell capable of expressing a sequence of interest, methods of expressing a sequence of interest comprising culturing the CHO cell as described herein, and kits comprising the CHO cell as described herein.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention provides an adaptive weight distribution chromosome stripe contrast enhancement method and system, and the method comprises the steps: firstly improving the significance of a chromosome stripe structure and an edge contour in a chromosome gray image through an edge enhancement convolution operator; carrying out chromosome monomer target detection and instance segmentation on the metaphase chromosome based on an instance segmentation network of deep learning to obtain a chromosome monomer ROI region; then, extracting a structure tensor response of the ROI region of the chromosome monomer, designing an adaptive enhancement weight of a histogram merging interval based on a normalized difference between a response value and global statistical information, and performing reconstruction and interpolation on a histogram of the image of the ROI region of the chromosome monomer; and finally, adaptively enhancing the contrast of the chromosome stripe through iterative histogram matching. According to the method, the original image texture structure is kept, and meanwhile, the definition and the distinguishability of chromosome stripes are effectively and adaptively improved.

The invention relates to a biological information data processing technology, in particular to a method for analyzing a single-cell Hi-C regulation scale chromatin band, which comprises the following steps: preprocessing single-cell Hi-C data to generate pseudo-batch Hi-C data; performing normalization processing on the pseudo batch Hi-C data to extract a Hi-C contact matrix of each chromosome; identifying and detecting false batch strips from the Hi-C contact matrix; projecting the pseudo batch strips to the original single cell Hi-C data to obtain single cell strips; and carrying out quantitative analysis on the single-cell strip in the original single-cell Hi-C data. According to the method, the regulation and control scale chromatin bands with definite endpoints and directivity can be stably identified, and a band set with remarkable statistics is output.

The present disclosure provides systems and methods for modulating the occurrence of chromosomal rearrangements, e.g., translocations, in a cell during genome editing. Embodiments are provided for reducing the occurrence of unwanted chromosomal rearrangements, and for increasing the occurrence of desired chromosomal rearrangements.

Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1 / 2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

To provide proteins for enhancing proliferation and / or healing and / or inhibiting apoptosis of non-transformed tissue or non-transformed cells.SOLUTION: The present invention provides proteins comprising amino acid sequences encoded by nucleic acids derived from a human chromosomal region C19Orf10 termed Factor 1 and / or C19Orf63 termed Factor 2, or inhibitors thereof. Further provided is use of inhibitors of Factor 1 and Factor 2 for medical use, preferably for use in treating or preventing a disease in which angiogenesis contributes to disease development or progression.SELECTED DRAWING: None

A method for detecting structural variations in chromosomes by labeling single-stranded chromatids with different colored probes is disclosed. The hybridization patterns of the labeled probes generate spectral profiles that enable high-resolution detection of structural variations, facilitating the distinction between benign and deleterious structural variations. Furthermore, the spectral profiles provide information about complex structural variations that may result from more than one rearrangement of chromosomal segments. The spectral profiles can be used to generate data tables, which can then be subjected to node analysis to identify structural features of interest.

It was discovered that by binding to CTCF which is important for maintaining chromosomal structures, hSATII RNA inhibits the function thereof, changes chromosome interaction, and induces transcription of inflammation-associated genes. It has also been discovered that cell death can be selectively induced in senescent cells and cancer cells by suppressing hSATII RNA expression. From the obtained results, it has been found that cancer treatment which targets senescent stromal cells and cancer cells can be performed with a substance that suppresses hSATII RNA or a substance that increases CTCF expression. In addition, cancer can be detected early by measuring the expression and activity of hSATII RNA and CTCF, and an epigenomic change of the hSATII DNA region. Thus, it is possible to provide a therapeutic drug for a cancer associated with cellular senescence, a method for screening thereof, and a test support method.

Disclosed herein is a non-chromosomal dynamic activity system (ADAS) derived from a parent bacterial cell comprising at least one loss of genetic function in a lyase to increase the stability of the ADAS. Also disclosed are methods of blocking sporogenesis in a parent bacterial cell in combination with a lyase deletion or other loss-of-function mutation.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Described herein are methods for making genetically modified cells by introducing combinations of genetic variants (designed or random) or constructs (genes or otherwise arbitrary DNA) into a population of cells, and for tracking each variant combination by sequentially building an array of barcodes at a common locus (chromosomal or plasmid), termed the barcode locus. Also described are the cells made by such methods.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

A multi-modal pan-cancer early prediction method is disclosed, comprising: collecting cfDNA samples of i cancer populations and healthy populations respectively and extracting methylation data; merging CpG sites based on the methylation data to obtain multiple methylation intervals, screening multiple candidate marker intervals from the multiple methylation intervals, and further screening the multiple candidate marker intervals; training a first prediction model using the multiple candidate marker intervals, training a second prediction model based on the fragmentation characteristics of the chromosomes extracted from the reference genome, and training a third prediction model by extracting the methylation entropy characteristics of the chromosomes; training a multi-modal pan-cancer early prediction model through the cancer population prediction values of the first prediction model, the second prediction model and the third prediction model, which can provide precise joint early screening for lung cancer, intestinal cancer, gastric cancer, liver cancer, esophageal cancer, thyroid cancer and ovarian cancer.

This invention relates generally to the discovery of an improved method to differentiate histiocytic malignancy from lymphoma or hemangiosarcoma in dogs.

Provided herein are, inter alia, are methods and systems for amplifying a chromosomal fragment DNA sequence. The methods and systems provided herein are, inter alia, useful for detecting copy number variation, structural variation, and / or extrachromosomal DNA within the chromosomal DNA of a plurality of cells. In addition, the methods provided herein are, inter alia, useful for jointly analyzing chromatin architecture and gene expression at a single cell level. The methods provided herein can be, inter alia, used to analyze chromosomal heterogeneity, clonal shifts, and / or evolutionary shifts between different cells.

Provided herein are achromosomal dynamic active systems comprising a Type 1 pilus (TIP) and methods of making and using the same.

The present application relates to biological information data processing technology, and is a method for single-cell Hi-C regulatory scale chromatin band analysis, comprising the following steps: preprocessing single-cell Hi-C data to generate pseudo-bulk Hi-C data; normalizing the pseudo-bulk Hi-C data to extract the Hi-C contact matrix of each chromosome; identifying and detecting pseudo-bulk bands from the Hi-C contact matrix; projecting the pseudo-bulk bands onto the original single-cell Hi-C data to obtain single-cell bands; and quantitatively analyzing the single-cell bands in the original single-cell Hi-C data. The present application can stably identify regulatory scale chromatin bands with clear endpoints and directionality, and output statistically significant band sets.

The present disclosure provides novel compositions and methods suitable for specifically eliminating target cells (e.g., cancer cells) without affecting non-target cells (e.g., non-cancer cells). For example, CRISPR system and the compositions of the present disclosure can be employed to specifically introduce a suicidal gene into a cancer cell in the loci of a cancer-specific target sequence, which as a result of chromosomal re-arrangement or translocation in a cancer cell presents a cancer specific sequence for a guide RNA and CAS to be recognized and such sequence is absent in a non-cancer cell. Consequently, the specific introduction of the composition(s) to cancer-specific site(s) and integration of suicide gene in the target genome, which is inapplicable to normal cells for lack of the site(s), leads to selective elimination of cancer cells but not non-cancer cells, and therefore render novel therapeutic methods and compositions for cancer treatment.

The application discloses a Pichia pastoris engineering bacterium for converting methanol to synthesize bakuchiol from scratch as well as construction and application of the bacterium. In different chromosomal neutral sites of the Pichia pastoris, a heterologous p-coumaric acid synthesis pathway is introduced, key genes of tyrosine and phenylalanine synthesis pathways of a heterologous shikimic acid pathway are knocked out, overexpressed or heterologously expressed, a high-yield p-coumaric acid strain is obtained, on the basis of the strain, a heterologous bakuchiol biosynthesis pathway is introduced, bakuchiol synthetase is overexpressed, key genes of a MVA pathway and genes of an exogenous acetyl coenzyme A supply pathway are endogenously overexpressed or heterologously expressed, after optimization of a methanol concentration and back complementation of a His4 gene of the strain, the yield of the bakuchiol is effectively increased to 91.2 mg / L, which is 59.8 times higher than that of an initial strain, and the yield of the bakuchiol in a 15L fermenter reaches 692.8 mg / L. The application has the characteristics of high conversion efficiency, low production cost, easy preparation, wide industrial application prospect and the like.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

The invention belongs to the technical field of bamboo reed breeding, and discloses a mutation breeding method for obtaining a bamboo reed cold-resistant mutant based on EMS (Enhanced Mass Spectrometry), which comprises the following steps: step 1, inducing embryogenic callus; step 2, carrying out EMS mutagenesis treatment; step 3, performing EMS stress screening; 4, differentiation and rooting culture of adventitious buds; 5, screening the tissue culture seedlings at low temperature; the mutation obtained through mutagenesis treatment is a permanent change of a genetic structure of an organism and may appear in germ cells and somatic cells of a plant. Physical or chemical mutagenic agents are used to increase the frequency of mutations. In chemical mutagens, EMS is considered to be the most effective mutagen, the EMS can generate a large number of non-lethal point mutations in the whole genome, the mutation frequency is high, the chromosome abnormality frequency is low, the EMS is used for treating embryogenic calluses of the bamboo reeds, Hyp is used for screening, the low-temperature-resistant bamboo reed mutants are obtained, the planting area of the bamboo reeds can be enlarged, and the survival rate of the bamboo reeds is increased. And waste land in a low-temperature region is fully utilized.

A transgenic Cory fish comprising a chromosomally integrated expression cassette encoding an orange fluorescent protein (OFP). The Cory comprises an orange Cory 1 transformation event, cryopreserved a

The invention discloses application of disulfiram in preparation of a medicine for treating UMOD gene mutation type autosomal dominant renal tubulointerstitial nephropathy, and belongs to the field of biomedicine. The application comprises the application of the disulfiram in preparation of the medicine for treating the hereditary tubulointerstitial nephropathy. According to the invention, a new pathogenic mutation UMOD c.106Cgt is identified; according to the invention, the pathogenicity of the gene is verified through a UMODH36Y mouse model constructed by CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated 9). DSF intervention is adopted, the serum creatinine and glomerular filtration rate (tGFR) of mice can be remarkably improved, and renal interstitial inflammation and fibrosis are relieved. The discovery prompts that the DSF realizes the therapeutic effect of renal function protection-inflammation inhibition-fibrosis relief in an ADTKD-UMOD animal model through targeted regulation of a pyroptosis pathway, a novel therapeutic strategy is provided for ADTKD-UMOD, and the clinical transformation period can be remarkably shortened due to the characteristic of new use of old medicines. The discovery lays an experimental foundation for developing a precise treatment scheme for the hereditary renal tubulointerstitial disease.

The invention discloses an embryo development quality evaluation method and device, and belongs to the field of embryo in-vitro culture, the embryo before implantation is subjected to three-dimensional imaging to form a three-dimensional blastocyst structure chart, fluorescence imaging is carried out to form a redox specific heat chart, the three-dimensional blastocyst structure chart and the redox specific heat chart are subjected to co-localization superposition, and the embryo development quality is evaluated. Constructing a three-dimensional blastocyst model based on map combination; according to the three-dimensional blastocyst model, the spatial form, chromosome ploidy and metabolic state of the early blastocyst are comprehensively evaluated, and non-intrusive, accurate and efficient blastocyst ectodevelopment quality evaluation and transplantation screening are achieved.

Methods of transferring large sequence fragments between chromosomes and generating chromosomal rearrangements using double strand break repair pathways and homology directed repair. Further relates to chromosomes produced by these methods, and cells and transgenic animals comprising these chromosomes.

It was revealed that by binding to CTCF which is important for maintaining chromosomal structures, hSATII RNA inhibits the function thereof, changes chromosome interaction, and induces transcription of inflammation-associated genes. It has also been discovered that cell death can be selectively induced in senescent cells and cancer cells by suppressing hSATII RNA expression. On the basis of the obtained results, a disease caused by cellular senescence can be treated with a substance that suppresses hSATII RNA or a substance that increases CTCF expression. Whether the disease is caused by cellular senescence can be diagnosed by measuring the expression of hSATII RNA and CTCF, and an epigenomic change of the hSATII DNA region. Thus, it is possible to provide a therapeutic drug for a disease caused by cellular senescence, a method for screening the therapeutic drug, and a method for acquiring data for detecting a disease caused by senescence.

A chromosomal aneuploidy analysis method and a use. The method comprises: using sequencing data of a sample to be tested and an alignment result thereof to calculate the fetal DNA concentration in said sample and the window depth of said sample within a set window; and calculating the average relative depth of all samples in a selected reference set within the set window as a correction baseline, using the correction baseline to correct the window depth of said sample, calculating a Z value of said sample on the basis of the corrected window depth, and on the basis of the Z value, determining whether an aneuploidy abnormality has occurred to a fetal chromosome of said sample. Using the selected reference set to calculate the correction baseline for correcting said sample enables more stable and accurate detection of the chromosomal aneuploidy abnormality in said sample under the condition that there are no samples in a same batch as references, thereby reducing inter-batch variability in testing.