24 results about "Chromosome regions" patented technology

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The application provides a method and system for detecting a homozygous region of a chromosome and an electronic device, and relates to the technical field of prenatal detection. The method comprises the following steps: dividing chromosomes of a sample to be tested into a plurality of chromosome regions according to a preset step length and selecting a target chromosome region; constructing a background joint probability distribution model and a target joint probability distribution model; determining a background parameter according to the background joint probability distribution model; determining a joint distribution likelihood value of an SNP site in the target chromosome region according to the target joint probability distribution model and the background parameter; and determining a homozygous condition of the target chromosome region according to the joint distribution likelihood value of the SNP site in the target chromosome region. The application can determine whether a fetus has a homozygous region of a chromosome based on non-invasive sampling and deduce a genetic source of the fetus by constructing the background joint probability distribution model and the target joint probability distribution model, thereby reducing the cost of detection and improving the safety of detection.

The invention discloses a cotton verticillium wilt resistance related gene GhACA12 and application thereof in disease-resistant breeding, and belongs to the technical field of agricultural biology. Through whole genome association analysis (GWAS), an SNP site D11: 57218252 related to verticillium wilt resistance is found in a cotton D11 chromosome region, and a GhACA12 gene is screened out as a candidate disease-resistant gene. Researches show that high expression of GhACA12 is related to susceptible characters, and low expression of GhACA12 is related to disease resistance characters. The GhACA12 gene is silenced through a VIGS technology, and the disease resistance of the cotton is remarkably enhanced. Specific primers are designed for qRT-PCR detection, and disease-resistant materials are screened in an auxiliary mode. The invention provides effective technical support for cotton verticillium wilt resistant molecular breeding, and has important application value.

The invention discloses a haplotype molecular marker related to cotton fiber strength and application of a detection primer of the haplotype molecular marker, and relates to the technical field of plant molecular biology and genetic breeding. The haplotype molecular marker is located in 112, 985, 431-112, 990 and 059bp regions of A06 chromosome of a cotton reference genome, a haplotype block is formed by nine closely linked SNP (Single Nucleotide Polymorphism) sites, the incidence relation between three haplotypes Hap1, Hap2 and Hap3 in the block and fiber strength is defined, and a new molecular target is provided for cotton fiber quality breeding. Compared with a single SNP marker, the haplotype molecular marker provided by the invention has higher stability and phenotype prediction accuracy under a complex genetic background. Genetic typing can be carried out in the cotton seedling stage based on the marker, fiber maturation does not need to be waited, the breeding period is greatly shortened, and the field screening cost is reduced.

The present application provides a marker combination for early detection of digestive system cancer, tissue localization, diagnosis, prognosis detection and identification of benign and malignant, the marker combination is selected from 1656 chromosome regions in table 1. A multi-cancer early screening and localization technology GutSeer for five high mortality digestive system cancers is also provided, which proves that using a relatively small second-generation sequencing panel can realize accurate cancer detection and organ tissue localization by using multiple dimensional characteristics including methylation, copy number variation and terminal motif.

The invention discloses an InDel molecular marker related to maize rough dwarf disease resistance and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker closely linked with the maize rough dwarf virus resistance gene is obtained by screening in 12, 857, 147-13, 124 and 718 bp regions of the maize chromosome 2, the resistance of maize to rough dwarf virus can be accurately identified in an early stage by utilizing the InDel molecular marker, and the InDel molecular marker has the advantages of simplicity, convenience, rapidness, high efficiency, accuracy, good repeatability, high specificity and the like; the method can be used for corn molecular marker-assisted breeding.

The present application relates to the technical field of plant breeding, and provides a corn molecular marker assisted backcross breeding electronic simulation method and system, the method comprising: collecting whole genome molecular marker data of a first group of single plants carrying known target genes which exhibit new traits; collecting whole genome molecular marker data of a donor parent of the known target genes; collecting whole genome molecular marker data of a recipient parent of the known target genes; based on the whole genome molecular marker data, deducing one or more candidate chromosome regions by analyzing chromosome fragments commonly inherited from the donor parent in the genome of the first group of single plants; selecting representative molecular markers from the candidate chromosome regions as proxy targets; taking the proxy targets and the whole genome molecular marker data of the first group of single plants as selection targets, performing electronic simulation of subsequent backcross breeding, and outputting the results of the electronic simulation. The present application has the effect of improving breeding efficiency and decision-making scientificity.

The invention relates to the technical field of plant molecular breeding, in particular to an indel marker PH-03-Idel-106 related to the plant height character of corn and application of the indel marker PH-03-Idel-106 related to the plant height character of the corn, the Indel marker is located in the area of the third chromosome Chr3: 163962052-163964052 of the corn and is a 7bp insertion / deletion polymorphism marker, a 7bp insertion sequence CTGAGGG exists in a high-stalk corn inbred line, and no insertion sequence exists in a dwarf corn inbred line. A matched specific primer pair PH-03-Indel-106F / R can specifically amplify a target area, 1.5% agarose gel electrophoresis detection is carried out, it is judged that the corn plant height marker is a dwarf haplotype when a 270bp band appears, it is judged that the corn plant height marker is a high-stalk haplotype when no band exists, and the marker can be used for molecular marker-assisted selection, breeding improvement and early identification and screening of the corn plant height character. Phenotypes can be accurately predicted through DNA detection in the seedling stage, and environmental interference is avoided. The method has the advantages of being high in specificity, simple and convenient to detect, low in cost, high in throughput and the like, the breeding period can be remarkably shortened, directional improvement of the plant height character is achieved, and the method has important significance on corn variety genetic breeding and plant type optimization.

This application provides a method and system for intelligent chromosome karyotype segmentation that integrates deep learning. The method involves: enhancing chromosome microscopic images; performing region segmentation based on the enhanced chromosome images to obtain multiple chromosome region sub-images; for any chromosome region sub-image, extracting the chromosome pixel area and band count corresponding to that sub-image, and determining the structural coverage index of the sub-image based on these values; comparing the structural coverage index with a preset chromosome banding distribution model to perform anomaly screening and identify candidate chromosome adhesion regions; and segmenting the candidate adhesion regions using a deep learning segmentation model to obtain the corresponding chromosome karyotype segmentation image. This application can screen and identify candidate adhesion regions based on the chromosome skeleton banding density, improving the accuracy of chromosome image segmentation.

The present invention provides a method for constructing an inbred mouse model by introducing a group of spontaneously mutated Hr genes and its application. The method is to introduce the target gene into the inbred strain by homologous introduction to obtain a new gene introduction animal model. First, the ICR-Hr from the outbred group with strong genetic polymorphism is introduced. hr (Trade name: SHJH hr ) were introduced into the inbred BALB / cAShjh mice to establish the BALB / cA.Cg.ICR‑Hr hr / Shjh mice, and then continued to be introduced into the inbred C57BL / 6JShjh mice to establish C57BL / 6J.Cg.BALB / cA-Hr hr / Shjh, thereby establishing a homologous introduction inbred line, each introduction needs to be repeated for 10 or more consecutive generations, and repeated backcrossing allows the recipient strain to replace the chromosomes of the donor strain with the chromosomes other than the chromosome region carrying the Hr allele, so that related applications can continue to be studied on the characteristics of each strain.

The invention discloses a chromosome counting method and device based on image processing and electronic equipment. The method comprises the steps that computer equipment acquires a chromosome karyotype graph; projecting each row of pixel points of the chromosome karyotype graph to obtain a first projection vector; determining a plurality of foreground regions of the chromosome karyotype graph according to the first projection vector; selecting a plurality of first foreground areas from the plurality of foreground areas, and extracting target image areas corresponding to the plurality of first foreground areas; projecting each column of pixel points of the target image area to obtain a second projection vector; determining one or more chromosome regions contained in the target image region according to the second projection vector; selecting a target chromosome region comprising the chromosomes of the target type from one or more chromosome regions contained in the target image region; and obtaining the chromosome number corresponding to the chromosome of the target type. According to the invention, the counting accuracy and efficiency of the chromosome karyotype graph can be improved.

The invention relates to the technical field of boar breeding, and discloses application of a molecular marker in boar sperm malformation heat stress resistance screening, the molecular marker is located in a CIB4 gene of a No.3 chromosome 112.2-113.2 Mb region, the mutation site is 112656241bp, and T is greater than C. The relationship between the molecular marker and the sperm malformation heat stress resistance of the boar is determined by application, and a guiding effect is provided for genetic breeding related to the sperm malformation heat stress resistance of the boar. Meanwhile, the invention also discloses a method for screening the molecular marker.

This invention relates to the field of genetic engineering technology, and more particularly to a molecular marker for detecting wheat leaf rust resistance genes LrN4B or LrN3B and its application. The molecular marker LrN4B-1 is located in the 578-585Mb region of chromosome 4BL, with a polymorphism of C / G. The molecular marker LrN3B-1 is located in the 4-8Mb region of chromosome 3BS, with a polymorphism of T / C. This invention obtains molecular markers linked to wheat leaf rust resistance genes LrN4B and LrN3B through the construction of genetic linkage maps and the screening and localization of molecular markers. These two molecular markers can be used to distinguish the LrN4B+LrN3B disease-resistant genotype, screen for wheat lines containing these two disease-resistant genes, accelerate the process of wheat disease-resistant breeding, and are of great significance in the field of breeding leaf rust-resistant wheat.

The invention discloses a KASP molecular marker linked with a loose cauliflower globule gene and application, and belongs to the technical field of molecular breeding. Specifically, a high-generation inbred line material and a globule mutant obtained through EMS mutagenesis of the high-generation inbred line material are utilized to construct a segregation population for gene localization, a chromosome region closely linked with globule characters is obtained, and a corresponding molecular marker is developed in a candidate region. A KASP molecular marker is further designed and verified for a key single base mutation site of a candidate gene. Through verification, in genotype identification of 550 single plants, the coincidence rate of the marker with large and small ball phenotypes reaches 100%. The molecular marker can be effectively applied to molecular marker-assisted selective breeding of loose broccoli with big and small ball characters, and the selection accuracy and the breeding efficiency are remarkably improved; meanwhile, an important tool and a genetic basis are provided for cloning and functional analysis of related genes of the globules.

The present invention discloses a method, apparatus, device, and medium for gender detection based on second-generation sequencing technology. The method comprises: excluding CNV mutation-prone regions and sex chromosome PAR regions from valid detection regions of second-generation sequencing to obtain a high-efficiency gender detection region; calculating the coverage depth ratio of sex chromosomes to autosomes in the high-efficiency gender detection region based on a sample sequencing data comparison file, and obtaining the sex chromosome copy number based on the coverage depth ratio; determining X chromosome heterozygosity in the high-efficiency gender detection region based on a sample sequencing data variation file; and obtaining a gender detection result based on the sex chromosome copy number and X chromosome heterozygosity based on a configuration file. Reliable and stable gender detection is achieved based on second-generation sequencing technology.

The invention provides a chromosome overlapping detection method and device based on cross-modal feature fusion, and the method comprises the following steps: obtaining a ten-time lens to-be-detected image, inputting the ten-time lens to-be-detected image into a chromosome region detection model, and obtaining a ten-time lens chromosome region, acquiring a corresponding hundred-fold mirror chromosome region based on the coordinates of the ten-fold mirror chromosome region, and splicing the feature map of the ten-fold mirror chromosome region and the feature map of the corresponding hundred-fold mirror chromosome region to obtain a chromosome detection map, and inputting the chromosome detection graph into a pre-trained chromosome overlapping detection model to obtain a chromosome overlapping detection result. According to the scheme, the feature map of the ten-fold mirror chromosome region and the feature map of the corresponding hundred-fold mirror chromosome region are spliced as the input image, so that the detection precision of the short chromosome is optimized.

The present application provides a chromosome overlap detection method and device based on cross-modal feature fusion, including the following steps: obtaining a ten-fold microscope image to be measured, inputting the ten-fold microscope image to be measured into a chromosome region detection model to obtain a ten-fold microscope chromosome region, obtaining a corresponding hundred-fold microscope chromosome region based on the coordinates of the ten-fold microscope chromosome region, splicing the feature map of the ten-fold microscope chromosome region and the feature map of the corresponding hundred-fold microscope chromosome region to obtain a chromosome detection map, and inputting the chromosome detection map into a pre-trained chromosome overlap detection model to obtain a chromosome overlap detection result. By splicing the feature map of the ten-fold microscope chromosome region and the feature map of the corresponding hundred-fold microscope chromosome region as the input image, the detection accuracy of short chromosomes is optimized in this solution.

The invention provides a copy number variation degree analysis method and device and a storage medium, and relates to the technical field of bioinformatics analysis. The method comprises the following steps: determining a copy number baseline of each chromosome region through a reference sample set; according to the copy number baseline of each chromosome region, determining a Z standard score of the corresponding window-level chromosome region, and summing the Z standard scores of which the absolute values are greater than a set threshold value to obtain a whole genome cumulative score; determining threshold values of normal samples and abnormal samples in the whole genome cumulative fraction by using an ROC curve method; after the to-be-detected sample is calculated according to the method to obtain the whole genome cumulative fraction, the result is judged according to the whole genome cumulative fraction threshold value, the risk that the analysis result deviates from reality can be reduced, and the reliability of the method in clinical application can be improved.

The application provides a pepper cytoplasmic male sterility fertility restoration molecular marker, a typing primer and application thereof, constructs an F2 population by using wild type and mutant pepper materials, obtains a chromosome region closely linked to a pepper cytoplasmic male sterility fertility restoration gene by using a BSA population positioning method, designs a KASP molecular marker according to single base mutation, and genotype of 1290 single plants in the F2 population is identified by using the marker, and the coincidence rate reaches 100%. The application not only contributes to identification and assisted breeding of the pepper cytoplasmic male sterility, but also provides a basis for map-based cloning of the cytoplasmic male sterility fertility restoration gene and analysis of a molecular mechanism of the cytoplasmic male sterility, and has wide popularization value.

The invention discloses a chromosome image enhancement method, system and device and a medium. The method comprises the following steps: acquiring a chromosome grayscale image; extracting a foreground image of the chromosome region-of-interest; the method comprises the following steps: initializing five control points and node vectors, and generating a cubic clared B spline curve; three middle control points of the five control points are dynamic control points; constructing an image gray mapping table based on a cubic classed B spline curve; mapping the foreground image of the region of interest of the chromosome according to an image gray mapping table to obtain an enhanced chromosome image; and acquiring the real-time positions of the three dynamic control points, updating the compressed B spline curve and the image gray mapping table for three times, and updating the enhanced chromosome image. According to the method, the calculation complexity is low, the positions of the three dynamic control points can be adjusted in real time, the enhanced chromosome image can be synchronously updated so that a user can confirm the chromosome image in time, and the efficiency is improved on the premise that the enhancement quality of the chromosome image is guaranteed.

A process for analysing chromosome regions and interactions relating to physical performance.

The application discloses a molecular marker linked to a bulbous stem enlargement gene of Brassica oleracea and application, and belongs to the technical field of molecular breeding. The application uses a small ball material of a breeding high-generation inbred line material of Brassica oleracea and an EMS mutant library thereof, obtains a chromosome region linked to the bulbous stem enlargement gene of Brassica oleracea based on BSA population positioning, and develops a molecular marker in a candidate interval. A KASP molecular marker is designed according to single base mutation of a candidate gene, and genotypes of 523 single plants are identified by using the marker, and a coincidence rate with a phenotype reaches 100%. The application can be directly used for molecular marker assisted breeding of the bulbous stem enlargement plant of Brassica oleracea, improves selection efficiency of breeding, and speeds up a breeding process; meanwhile, lays a foundation for cloning, function verification and metamorphic stem growth and development of the enlargement gene Bofse-1 and related researches.

The invention relates to a cancer early screening related methylation marker targeted capture detection method and application thereof.The cancer early screening related methylation marker targeted capture detection method is characterized in that based on methylation markers of 1666 chromosome regions, targeted methylation gene detection is carried out on a sample by adopting a non-bisulfite treatment and liquid phase hybridization capture combined mode, and by screening and analyzing sequencing data, the methylation marker related to cancer early screening is obtained. And a model is established for early screening and diagnosis of cancers and residual examination of small focuses, so that accurate early screening of the cancers is realized.

The invention provides a method and a device for detecting chromosome arm and gene copy number variation of a central nervous system tissue FFPE sample. According to the method, a clinical retrospective FFPE sample of a central nervous system tumor is used, areas with neutral non-copy numbers are removed through cross validation, a sequencing depth distribution model with neutral copy numbers is constructed, and the copy numbers are used as a negative copy number reference when the copy numbers of the tumor sample are calculated; and the copy number fluctuation caused by technical noise in the FFPE preparation or experiment process is reduced. In addition, multiple Gaussian distribution components are fitted for copy number variation data of a central nervous system tumor clinical sample, a classification model is constructed through unsupervised learning to delimit positive thresholds of CNV amplification and deletion of the clinical sample, and the accuracy of a detection result of a target CNV gene or chromosome region is improved.