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Molecular marker interlinked with major QTL qFSW-2 and qFSW-5 for storage capability of corn seeds and application

A technology of seed storage stability and molecular markers, applied in the field of molecular biology, can solve the problem of few QTLs analysis

Active Publication Date: 2017-11-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above studies were mainly conducted on the morphological indicators related to germination, and the QTLs analysis on the physiological indicators of seed storability is relatively small.

Method used

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  • Molecular marker interlinked with major QTL qFSW-2 and qFSW-5 for storage capability of corn seeds and application
  • Molecular marker interlinked with major QTL qFSW-2 and qFSW-5 for storage capability of corn seeds and application
  • Molecular marker interlinked with major QTL qFSW-2 and qFSW-5 for storage capability of corn seeds and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Establishment and Genotype Analysis of Genetic Research Groups for Corn Seed Storage-Related Traits

[0041] 1. Test material

[0042] Two maize inbred lines (Table 1) with significant difference in seed storage tolerance were selected and bred in the early stage. Among them, Dong 156 was resistant to storage, and the germination rate remained above 90% under natural storage conditions for 8 years; Dong 237 was not resistant. Storage, under natural conditions, the germination rate drops to about 80% for one year. The experimental materials and seeds were provided by the Maize Research Institute of Northeast Agricultural University.

[0043] Table 1 Information about two maize inbred lines with significant differences in storability

[0044]

[0045] 2 test method

[0046] 2.1F 2 Colony construction and material planting

[0047] In the spring of 2011, East 156×Dong 237 was prepared in Harbin, and F was planted in Hainan in autumn 2011 1 , F 2 seed. ...

Embodiment 2

[0093] Example 2 Phenotypic Determination of Corn Seed Storage-Related Characters

[0094] 1 test material

[0095] Prepared East 156 x East 237F 2 There are 267 individual plants in the population.

[0096] 2 test method

[0097] 2.1 Artificial aging treatment

[0098] Randomly take out 150 seeds from each individual plant, repeat 3 times, repeat 50 seeds each time, disinfect with 1% sodium hypochlorite disinfectant for 30 minutes, put them into a small mesh bag, adjust the water bath to 58°C for 10 minutes, and put Put the small mesh bag into the water bath for aging for 60 minutes. After aging, the small mesh bag was taken out from the water bath, rinsed twice with tap water and then washed twice with distilled water. Put the rinsed seeds to dry at room temperature for 2-3 days to balance the water and then measure the related indexes of seed vigor.

[0099] 2.2 Standard germination test

[0100] The standard germination test refers to the germination method specified ...

Embodiment 3

[0110] Example 3 Analysis of QTL for Corn Seed Storability and Development of Molecular Markers

[0111] 1 test material

[0112] On Microsoft excel, the SSR marker genotype database of 267 individual plants in the mapping population, F 2 ： 3 The phenotype database of indicators related to seed storage tolerance, including germination rate, germination potential, seedling fresh weight, germination index, and vigor index (the average value of 3 repetitions for each index).

[0113] 2 methods

[0114] 2.1 Construction of SSR map

[0115] Icimapping 4.0 software (2014) was used to construct the genetic linkage map of the SSR marker loci of the F2 population. First, use the "group" command to group some markers, and then use the "order" command to determine the sequence of markers in each linkage group (LOD=3.0 ). The "Kosambi" function was used to convert the recombination values ​​into map distances (cM). Referring to the maize SSR Bin map, use the "map" command to constr...

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Abstract

The invention relates to a molecular marker interlinked with major QTL qFSW-2 and qFSW-5 for storage capability of corn seeds and application. The major QTL for the storage capability of the corn seeds is qFSW-2 in a Bin2.06 region of No.2 chromosome of corns and qFSW-5 in a Bin5.03 region of No.5 chromosome of the corns, wherein the molecular marker closely interlinked with qFSW-2 includes umc1079 and umc1028; and the molecular marker closely interlinked with qFSW-5 includes umc2063 and umc2400. According to the molecular marker, by positioning two major QTL sites, namely qFSW-2 and qFSW-5, for the storage capability of the corn seeds, four SSR molecular markers closely interlinked with the two major QTL are found, so that a feasible technological approach is provided for the molecular breeding for storable character of the corn seeds.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to molecular markers linked to main QTLs qFSW-2 and qFSW-5 of maize seed storage tolerance and their applications. Background technique [0002] The storability of corn seeds is of great value for the safe storage of seeds. The annual demand for corn seeds in my country is greater than 1.57 million tons, but the actual annual production is far greater than the consumption, and a certain amount of seeds must be stored. A large number of seeds require short-term or long-term storage. At present, the low-temperature storage method is generally used, but even if the low-temperature storage is used, the seed vigor decreases year by year, and finally loses its seed value. Studies have shown that the storage resistance of maize varieties is quite different, and some varieties still maintain a high germination rate after years of storage. , but the germination rate of some seeds is signific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王振华邸宏刘昭军张林周羽曾兴鲁忠全张先宇
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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