Indel molecular marker closely linked with capsicum annuum clustered inflorescence genes, primers and application

A technology of molecular markers and inflorescences, which is applied in the fields of capsicum breeding and molecular biology, can solve problems such as weak genetic research, unresolved mechanism of plant type and structure regulation, and few capsicum plant types, so as to improve efficiency and accuracy , Reduce the planting scale and the workload of later identification, and solve the effect of long identification period

Active Publication Date: 2019-12-20
HUNAN AGRICULTURAL UNIV +1
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Problems solved by technology

[0004] Although the important value of plant structure to crop production is well known, the genetic basis of its growth habit variation is relativ...

Method used

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  • Indel molecular marker closely linked with capsicum annuum clustered inflorescence genes, primers and application
  • Indel molecular marker closely linked with capsicum annuum clustered inflorescence genes, primers and application
  • Indel molecular marker closely linked with capsicum annuum clustered inflorescence genes, primers and application

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Experimental program
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Effect test

Embodiment 1

[0036] A pepper cluster molecular marker InDel-9258 was obtained by the following method:

[0037] 1. Group building

[0038] Use pepper LS20 solitary inbred line as the female parent, XJ10 pepper cluster inbred line as the male parent to obtain F1, F1 self-bred to obtain F2, complete F2 population construction ( figure 1 ).

[0039] 2. Inflorescence phenotype identification

[0040] (1) Parents of LS20 and XJ10, F1 and F2 populations were sown in plugs, and transplanted in glass greenhouses after seedlings were grown and managed by conventional cultivation.

[0041] (2) Perform phenotypic identification of F2 population when it is in full bloom. At the same time, 30 F2 plants with solitary and clustered inflorescences are selected to construct two mixed pools.

[0042] 3. QTL analysis of clustered inflorescence

[0043] Extract RNA from single flower buds of selected mixed pools, mix the same amount to construct two cDNA libraries, and sequence them on the IlluminaHiseq4000 platform. The...

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Abstract

The invention discloses an Indel molecular marker closely linked with capsicum annuum clustered inflorescence genes, primers and application. An F2 population is constructed by utilizing two highly-homozygous inbred lines. A chromosome region closely linked with capsicum annuum clustered inflorescences is acquired by using a BSR and linkage positioning method, and an InDel marker is developed in acandidate interval for verification. Finally, InDel closely linked with the vicinity of a candidate gene is obtained, a molecular marker In-9258 is designed according to the InDel, genotype identification is conducted on 100 randomly-sampled single plants of the F2 population by using the marker, and the coincidence rate reaches 100%. The research result not only can be used for capsicum annuum inflorescence type identification and molecular assisted breeding, but also provides a basis for controlling map-based cloning of capsicum annuum clustered inflorescence genes and for analyzing the forming molecular mechanism of clustered inflorescences.

Description

Technical field [0001] The invention belongs to the field of pepper breeding and molecular biology, and more specifically relates to an Indel molecular marker and primer closely related to pepper cluster inflorescence, and also relates to their application in pepper inflorescence prediction and molecular assisted breeding. Background technique [0002] Pepper (Capsicum annuum L.) is a vegetable crop of the Capsicum genus Solanaceae, which is native to Central and South America. Because of its rich nutritional value and strong adaptability, pepper is widely cultivated worldwide as an important vegetable crop and is popular among consumers. According to the statistics of FAO in 2014, the global output of peppers has exceeded 49 million tons. According to the statistics of the bulk vegetable system of the Ministry of Agriculture of China, the area of ​​pepper cultivation in my country has been increasing, and the pepper industry has made great progress. In recent years, the annual s...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6895C12Q1/6806C12Q2600/13C12Q2600/156
Inventor 欧立军吕俊恒刘周斌刘宇华陈文超邹学校于会洋陈蓉欧阳波李峰
Owner HUNAN AGRICULTURAL UNIV
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