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Molecular marker linked with pepper fruit cuticle deficiency genes and application thereof

A molecular marker and cuticle technology, applied in the field of pepper breeding molecular biology, can solve the problem of lack of fruit cuticle research, and achieve the effects of reducing the planting scale, reducing the workload, and solving the problem of long cycle.

Active Publication Date: 2020-08-21
HUNAN AGRICULTURAL UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on pepper fruit development, new variety breeding, disease resistance and insect resistance research, protected cultivation, capsanthin, capsaicin and the antioxidant of its extracts, but research on fruit cuticles is still lacking. As an important germplasm resource, fruit cuticle-deficient mutants will be favored by future breeders

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  • Molecular marker linked with pepper fruit cuticle deficiency genes and application thereof
  • Molecular marker linked with pepper fruit cuticle deficiency genes and application thereof
  • Molecular marker linked with pepper fruit cuticle deficiency genes and application thereof

Examples

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Embodiment 1

[0041] Example 1 Acquisition of Molecular Marker BSA Molecular Marker Linked to Synthetic Gene of Capsicum Fruit Cuticle

[0042] BSA positioning method

[0043] 1. Group construction

[0044] Using the backbone pepper parent '8214 ( figure 1 Shown in A), and the stable fruit cuticle-deficient mutant material '228' obtained from '8214' through EMS mutagenesis and continuous selfing for multiple generations was the parent ( figure 1 Shown in B), the backbone parent was crossed with the fruit cuticle-deficient parent to obtain F 1 Generation, F 1 F 2 group.

[0045] 2. Fruit cuticle identification

[0046] to be F 2 Observation and identification of the presence or absence of cuticles on the fruit surface during the green ripening stage of the fruit of the generation group was carried out to determine the cuticle of the fruit of a single plant in the group.

[0047] 3. Preliminary mapping of cuticle synthesis genes

[0048] at 8214 x 228 f 2 25 leaves of wild plants with...

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Abstract

The invention discloses a molecular marker linked with a pepper fruit cuticle synthesis gene and an application thereof. The F2 population is constructed by using wild type and mutant type chili materials. A chromosome region closely linked with a pepper fruit cuticle synthesis gene is obtained by using a BSA group positioning method, and a molecular marker is developed in a candidate interval. AnAKASP molecular marker is designed according to single base mutation, the marker is utilized to perform genotype identification on 182 single plants in the F2 population, and the coincidence rate reaches 100%. The research results not only are beneficial to identification and assisted breeding of the fruit cuticle deficiency type pepper, but also provide a basis for map-based cloning of fruit cuticle synthesis genes and analysis of a molecular mechanism of cuticle synthesis, and have a wide popularization value.

Description

technical field [0001] The invention belongs to the field of capsicum breeding molecular biology, and relates to a molecular marker linked to a synthetic gene of capsicum (Capsicum annuum L) fruit cuticle and the application of the molecular mark in identification and breeding of capsicum fruit cuticle. Background technique [0002] During the Middle Ages, about 450 million years ago, when plants began to colonize arid habitats, the greatest challenge faced by land plants was desiccation, so the most critical evolutionary innovation that allowed plants to colonize land was the introduction of a hydrophobic skin or cuticle The form develops specialized epidermal cell walls to limit transpirational water loss. Therefore, cuticles are present on the outer surfaces of almost all above-ground organs of land plants. In addition to preventing water loss, the cuticle, which is the primary protective barrier of plants, also plays other important physiological roles, such as resistin...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘峰谢玲玲易婷郑井元周书栋马艳青戴雄泽邹学校
Owner HUNAN AGRICULTURAL UNIV
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