99 results about "Monosomal karyotype" patented technology

The invention relates to a method for cultivating autologous umbilical cord mesenchymal stem cells by adopting human umbilical cord blood rich platelet lysate. The conventional cell bank preparing processes adopt blood serum of calves or fetal calves to cultivate, digest, cryopreserve and the like, so that the risk of heterogeneous protein residue certainly exists in the application of clinic umbilical cord stem cells in the future. The method comprises the following steps of umbilical cord blood rich platelet serum preparation, platelet lysate preparation, preparation of serous without platelet, the confirmation of the content of cell factors, namely PDGF-AB, FGF2, TGF-Beta and VFGF in the platelet lysate, the separation and the primary culture of umbilical cord stem cells, the culture and passage of umbilical cord stem cells, the cryopreservation of umbilical cord stem cells, and the karyotype analysis. The method is used for cultivating stem cells.

The invention discloses a chromosome karyotype image cutting method, and belongs to the technical field of chromosome image processing. An existing image segmentation method is used for segmenting a chromosome image, due to the fact that the chromosome image has a lot of noise, the phenomenon of wrong segmentation can occur, and errors are caused to bring interference to subsequent analysis. According to the method, chromosome image segmentation is mainly carried out based on connected domain segmentation and skeleton extraction modes, after independent single chromosomes are extracted throughconnected domain analysis, skeleton extraction and skeleton analysis are carried out on remaining cross chromosomes to find possible segmentation points, and segmentation of the cross chromosomes isrealized through the segmentation points; compared with the prior art, direct segmentation of the preprocessed image is changed, the segmentation effect is clear, errors caused by segmentation are reduced to the maximum extent, the process is simple and reasonable, large-scale popularization and application can be conducted outwards, and deployment is simple.

The invention provides an automatic chromosome karyotype analysis and anomaly detection method. According to the method, an attention mechanism and a convolutional neural network are combined, the target is positioned in two stages, and the area of the target is roughly positioned in the first stage; in the second stage, an attention mechanism is added to the target area in the first stage, deepersemantic features are extracted to predict a mask of the target, meanwhile, the position of the target is roughly positioned by means of category prediction and a detection box regression task, and segmentation is conducted; finally, the trained model is used for segmenting and detecting the chromosome image, chromosome segmentation and anomaly detection can be accurately achieved, and thereforechromosome karyotype analysis automation is achieved. The method provided by the invention can overcome the defects that a traditional chromosome karyotype analysis method mainly depends on genetics to carry out artificial interaction analysis, time is consumed, overlapped chromosomes are not easy to separate, abnormal chromosomes cannot be automatically detected and the like.

The invention discloses a nuclear acid probe marked with quantum dots and a preparation method and application thereof. Probes with different sequences are marked with quantum dots with different emission spectrum characteristics; the preparation method of the probe has the following steps: synthesizing numerous probes with different sequences and marking the probes with X; synthesizing numerous quantum dots with different emission spectrum characteristics and marking the dots with Y; integrating probes marked with X and quantum dots marked with Y through specificity of X and Y and carrying out coupling on the probes and the quantum dots; facilitating probes with different sequences to be marked with quantum dots with different emission spectrum characteristics; the probes are used for multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, lower detection cost, increase detection speed and prevent false positive result from occurring.

The invention discloses a chromosome identification method based on depth learning, belonging to the technical field of chromosome identification. At present, the method of chromosome analysis is basically manual operation, and the examiner needs a lot of training time to master the knowledge of identifying each chromosome type, and the workload is heavier. Even experienced physicians analyze andidentify patients' chromosomes, the process typically takes more than two weeks and takes a longer period of time. And artificial recognition, subjectivity is very strong, easy to be affected by the external environment, the accuracy is not high. As that depth learn method is adopted, the chromosome type can be accurately carry out, Compared with the existing identification technology, the high-efficient identification technology can effectively improve the efficiency of chromosome karyotype analysis, shorten the identification sorting time, complete the automatic classification and sorting ofchromosomes with high accuracy, at the same time, can effectively reduce the work burden of doctors, free from external interference, and the process is simple, reasonable, and can be applied to large-scale outward promotion.

The invention discloses a chromosome sorting method based on stripe recognition, and belongs to the technical field of chromosome image processing. According to an existing chromosome sequencing method, due to the fact that manual recognition is prone to interference, selection and arrangement accuracy is low, and structural comparison of doctors is affected. The method comprises the following steps: step 1, obtaining a chromosome karyotype diagram; step 2, carrying out chromosome cutting on the karyotype diagram to obtain a plurality of chromosome images, and identifying chromosome types; 3,extracting a plurality of images of chromosomes of the same number, and identifying chromosome stripe types based on an intelligent algorithm model; and step 4, sorting the plurality of divided chromosome images according to the chromosome stripe type changes of the chromosome images. The strip types of the chromosome images can be predicted, the chromosome strip number can be accurately recognized, reasonable sorting is performed according to the chromosome strip number, a doctor can conveniently observe the chromosome structure, the burden of the doctor can be effectively reduced, and time and labor are saved.

The invention discloses a preparation method of a marrow G-band for karyotype analysis in the medical examination field. The method includes adding ethidium bromide and demecolcine into a medium at the end of culture. The adding of a certain amount of ethidium bromide (EB) at the end of cell culture can make the chromosome in a split phase longer and the band patterns clearer. With the advantages of time saving, labor saving, and higher accuracy, the marrow G-band has more extensive application prospects in the field of medical examination.

The invention provides a denoising method for a chromosome G banding mid-term gray level image. The method provides a local threshold segmentation scheme, ingenious integration and optimization can beperformed on a classic image processing algorithm, and a method which has adaptability and can automatically process a chromosome image can be formed. For a data set with 5000 chromosome G banding mid-term gray level images, mutual comparison is performed on a processing result of the method and a manual processing result of a medical worker and the automatic processing accuracy of the method canreach 86.38%. Image reading pressure of the medical personnel can be greatly reduced, and the chromosome karyotyping work efficiency is reduced.

The invention discloses a G-banded adherent chromosome segmentation method based on geometrical feature and region fusion, At first, that concave point of the region outline of the adherent chromosomeare extracted, then the image of the adherent chromosome is cut by a cutting line for by two concave points, and finally, the local region aft the cutting is fused, and the most suitable region is selected as the final single chromosome region. The invention realizes automatic division of adherent chromosomes and improves the efficiency of chromosome karyotype analysis.

The invention relates to a human peripheral blood lymphocytes culture medium and the application thereof. The human peripheral blood lymphocytes culture medium of the invention comprises: RPMI1640 liquid culture medium 77-81.9%, bovine blood 10-14.9%, PHA 4-9%, non-sulfated glycosaminoglycan 2-5%, CPPs 2-5% and Double-resist 0.05-0.1%. The culture medium provided in the invention achieves a high lymphocyte conversion rate, a high lymphocyte split index and a good reagent stability; can obtain a human chromosome figure which can provide a firm basis to chromosome core type analysis and clinic diagnosis.

The invention relates to a biological agent and particularly relates to a lymphocyte culture medium. The lymphocyte culture medium comprises lectin, a basal culture medium and a serum substitution, wherein the lectin is L-type phytohemagglutinin; the basal culture medium is an RPMI1640 basal culture medium; and the serum substitution comprises bovine serum albumin, recombinant human insulin, ferric citrate and an amino acid mother solution. By adopting the serum-free human peripheral blood lymphocyte culture medium, the animal or human serum is not used so that the cost of the culture medium is reduced and the disease dissemination risk is also reduced. The components of the serum-free human peripheral blood lymphocyte culture medium are determined so that the problems of complex traditional serum components, quality differences in different batches and fluctuated culture effect can be avoided. The culture medium provided by the invention is high in lymphocyte conversion rate and lymphocyte metaphase index, low in reagent cost and stable in quality, and the cultured cell can be used for clinical diagnosis of the karyotype analysis.

The invention discloses a production method of triploid pseudobagrus fulvidraco, which belongs to the fish culture technology field. Artificial insemination is performed to the pseudobagrus fulvidraco female parent and male parent which are obtained through artificial induced spawning, after 10 to 15 minutes, triploid pseudobagrus fulvidraco is obtained through induction, and the duration time for induction is 10 to 20 minutes. After the triploid pseudobagrus fulvidraco is obtained through induction, the multiple of the chromosome is measured through the technologies, such as the karyotype analysis of chromosome, the flow cytometer, etc., and the triploid pseudobagrus fulvidraco is identified and sieved for culture. The method restrains the releasing time of the second polar body through time control on the artificial insemination, the induction through the physical or chemical method, and control on the induction duration time, and thereby the inductivity and the survival rate of the triploid pseudobagrus fulvidraco are greatly increased, and according to the experiment statistics, the artificial inductivity of the achieved triploid pseudobagrus fulvidraco is more than 50 percent, and the survival rate is more than 80 percent.

The invention provides a preparation method of a placenta source matrix mesenchymal stem cell. The preparation method comprises the following steps: (1) separating the placenta source matrix mesenchymal stem cell; (2) cultivating the placenta source matrix mesenchymal stem cell, wherein a trypLE<TM> enzyme solution is utilized to process a placenta tissue in two steps in the step (1), and a serum-free medium is utilized in the step (2). By adopting the method provided by the invention, the placenta source matrix mesenchymal stem cell is a complete matrix source by short tandem repeat sequence (STR) atlas analysis, and is qualified by chromosome karyotype examination, pathogenic microorganism examination of bacteria, funguses and viruses, cell purity identification and cell biology function identification. Therefore, the placenta source matrix mesenchymal stem cell obtained by the method provided by the invention does not contain foreign protein, can meet the requirements of clinical use, is especially suitable for use by mothers, and also provides a technical support for building an excellent placenta source matrix mesenchymal stem cell bank.

The invention relates to a chromosome slide preparing method which comprises the following steps of: providing a chromosome sample, putting a slide into water and keeping for more than 2 hours at 2-8 DEG C, carrying out pretreatment on the slide, fixing the chromosome sample, and dripping on the slide. The chromosome obtained by using the improved chromosome slide preparing method is sufficient in dispersion, free of overlapping, free of loss, clear in strip and regular in morphology; and the analysis on chromosome karyotype and the diagnosis on diseases are facilitated.

The invention relates to a chromosome slide preparing method which comprises the following steps of: providing a chromosome sample, putting a slide into water and keeping for more than 2 hours at 2-8 DEG C, carrying out pretreatment on the slide, fixing the chromosome sample, and dripping on the slide. The chromosome obtained by using the improved chromosome slide preparing method is sufficient in dispersion, free of overlapping, free of loss, clear in strip and regular in morphology; and the analysis on chromosome karyotype and the diagnosis on diseases are facilitated.

The invention discloses a nuclear acid probe marked with quantum-dots composite sphere and a preparation method and application thereof. Nuclear acid probes with different sequences are marked with quantum-dots composite sphere with different emission spectrum characteristics; the quantum-dots composite sphere comprises a sphere and a quantum-dots composite contained in the sphere, the quantum-dots composite is composed of randomly selected m types of quantum-dots with different emission spectrum characteristics from n types thereof, n and m are both positive whole numbers and m is less than or equal to n; the preparation method of the nuclear acid probe comprises the following steps: preparing numerous probes with different sequences and marking the probes with X; preparing numerous quantum-dots composite sphere with different emission spectrum characteristics and marking the sphere with Y; integrating probes marked with X and quantum-dots composite sphere marked with Y through specificity of X and Y and carrying out coupling on the probes and sphere; applied to multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, the probeof the invention can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, increase detection speed and prevent false positive result from occurring.

The invention discloses a frankliniella occidentalis arrhenotoky inbreeding population establishing method. According to the method, frankliniella occidentalis artificially bred is transferred into a centrifugal tube for single parthenogenetic breeding to establish an inbreeding population by controlling temperature, moisture and illumination manually. The method is characterized in that the frankliniella occidentalis arrhenotoky inbreeding population can be established, and obtained male offspring and multiple continuous generations of male and female offspring can be applied to karyotype analysis, microsatellite analysis and other experiments. The method has great significance for further learning the frankliniella occidentalis arrhenotoky features and establishing the inbreeding population and can further promote the analysis of genetic structure and sex determination mode.

The invention discloses a method for quickly establishing a metaphase chromosome karyotype of a cucumber through a genomic in-situ hybridization technology. The method includes the steps that genome DNA of the cucumber is extracted and marked as a probe; enzymolysis is conducted on an extracted root tip of the cucumber, a sheet is prepared through a flame drying method, and then a metaphase chromosome sheet with the good dispersity is acquired; the genome DNA serving as the probe is degenerated and then hybridized to the degenerated metaphase chromosome sheet, under the hybridization condition with the high preciseness, clear and differentiable fluorescence signals are produced through a chromosome tandem repeated sequences, and then the metaphase chromosome karyotype of the cucumber is established. The genome DNA of the cucumber serves as the probe, the metaphase chromosome karyotype of the cucumber is quickly established through one-time GISH, and then testing efficiency is improved. Through the method, based on the different signal distribution modes of the genome DNA probe of the cucumber on chromosomes, the metaphase chromosome karyotype of the cucumber can be quickly established, and a novel method and reference are provided for analytical investigation of karyotypes of species.

A spontaneous transferring cell strain SPC-A-1BM is taken as human-lung adenocarcinoma SPC-A-1 cell strain. It has high spontaneous transferring rate and missed rate, lung transferring rate reaches to 80%, nodus lymphoideus transferring rate reaches to 85% and bone transferring rate reaches to 25%. It has excellent DNA cell stability and can be used to make research for human-lung adenocarcinoma transferring mechanism.

The invention discloses a chromosome preparation method, as well as a required culture medium and a preparation method thereof, and is used for solving karyotype analysis problem of chromosome. The culture medium consists of RPMI (Roswell Park Memorial Institute) 1640, heparin sodium, HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid), L-glutamine, NaHCO3, benzylpenicillin potassium, streptomycin sulphate, bovine serum and phytohemagglutinin (PHA). The detection method comprises the following steps: implanting 0.3 to 0.4ml of human peripheral blood into the culture medium; adding colchicinamide in 2-4 hours before culture is terminated to realize that the cell is terminated in anaphase; culturing the cell after 68 to 72 hours to harvest the cell; performing hypotonicity for 40 minutes, three times of fixation, banding, dyeing and other treatments; and performing chromosome analysis under a microscope to determine whether the peripheral blood supplier has a phenomenon of chromosome abnormality. The culture medium disclosed by the invention has convenience for use, simpleness in operation, low cost and low patient detection fee, and is suitable for genetic diagnosis, infertility and prenatal diagnosis in each level of hospitals.

The separation and cultivation method for fish embryonic cell comprises: collecting cell; in CM, separating the cell of blastula-archenteron late time with laceration method, and culturing the initial and passage cells; identifying the cell on morphology, proliferation feature and chromosome karyotype. Wherein, using 70% alcohol to dip and sterilize for embryo; culturing cell in common incubator with 24Deg to passage 2-3 times every week. The objective cell is litle, circle or polygon, and growing stably, and has high rate of diploid cell. This invention is simple and convenient, and can be applied into separation and cultivation for almost all fish embryonic cell.

The invention discloses a detection method of aneuploid hybrid progeny plants. The method comprises the following steps: firstly performing preliminary assessment of ploidy levels of hybrid progeny plants; by using progeny plants and parental DNAs as a template, performing PCR amplification respectively; respectively performing fluorescence capillary electrophoresis on amplified products; finally, by taking parental genes as reference and combining the preliminarily assessed ploidy levels of the plants, identifying the number and height of obtained allele peaks, comparing the composition conditions of the alleles of the progeny plants, and if the number of marker sites positioned on a certain chromosome is more than or less than the expected number, determining to be aneuploid plants. The method provided by the invention can be used for high-throughput, rapid and accurate detection of aneuploid plants, and has an important significance on the study on aneuploidy hybrid genetic improvement and chromosomal operation. Compared with the traditional karyotype analysis method, the method has the advantages of low cost, easiness in operation, rapid detection speed, strong reliability and the like, and is suitable for analysis of large-scale mass materials.

This invention opens one establishing method of one fetal pancreas stem cell lines. epithelioid stem cell of fetal pancreas comes from fetal pancreas organization in normal abortion, in vitro culture can be clonned infinitely, and possesses the potential of multi-directional differentiation; after testing and analysis of morphological character, growth characteristics, immunohistochemistry characteristics, RT-PCR character, directional induced differectiation characters, chromosome karyotypes, DNA array, it is proved that this fetal pancreas stem cell is human originated fetal pancreas stem cell; this invention can provide affulent seed cells for researches on mammalian pancreas development and diabetes origination and dibetes treatment by reproducing Langerhans' islands' micro organs. It also provides an efficient and reliable method for establishing pancreas stem cell lines of creodonts and human beings.

The invention relates to a method for detecting chromosome abnormality before embryo implantation. Specifically, the detection accuracy is improved by adopting the steps of improving a single cell proliferation method and optimizing traditional library construction, and the false positive rate of a karyotype and a CNV is reduced.

The invention relates to a method for cultivating autologous umbilical cord mesenchymal stem cells by adopting human umbilical cord blood rich platelet lysate. The conventional cell bank preparing processes adopt blood serum of calves or fetal calves to cultivate, digest, cryopreserve and the like, so that the risk of heterogeneous protein residue certainly exists in the application of clinic umbilical cord stem cells in the future. The method comprises the following steps of umbilical cord blood rich platelet serum preparation, platelet lysate preparation, preparation of serous without platelet, the confirmation of the content of cell factors, namely PDGF-AB, FGF2, TGF-Beta and VFGF in the platelet lysate, the separation and the primary culture of umbilical cord stem cells, the culture and passage of umbilical cord stem cells, the cryopreservation of umbilical cord stem cells, and the karyotype analysis. The method is used for cultivating stem cells.

The invention discloses a human peripheral blood chromosome synchronization preparation kit which comprises thymidine, 2'-deoxycytidine, colchicine, a 1640 culture medium and a fixing solution. According to the kit, growth cycles of peripheral blood lymphocytes are synchronized, enough 500-550 chromosome karyotypes with stripes can be obtained, so that chromosomal aberration and small abnormal structures are accurately identified. The synchronization kit adopting excessive thymidine single occlusion, deoxycytidine releasing and ethanol acetic acid harvesting is capable of increasing resolution ratio, simple in operation steps, capable of reducing the contact frequency of toxic reagents, and suitable for wide and conventional application.

The invention discloses noninvasive prenatal gene testing and analyzing software. The software comprises a data quality control module, a data comparison module, a noninvasive prenatal analysis module, a data statistics module and a report generating module which are connected in sequence, and finally an analysis can be obtained, wherein the noninvasive prenatal analysis module comprises a GC correction module. The software is installed and used simply, is higher in accuracy compared with a conventional testing method and can analyze 23 pairs of karyotypes.

The invention discloses a nuclear acid probe marked with quantum-dots composite sphere and a preparation method and application thereof. Nuclear acid probes with different sequences are marked with quantum-dots composite sphere with different emission spectrum characteristics; the quantum-dots composite sphere comprises a sphere and a quantum-dots composite contained in the sphere, the quantum-dots composite is composed of randomly selected m types of quantum-dots with different emission spectrum characteristics from n types thereof, n and m are both positive whole numbers and m is less than or equal to n; the preparation method of the nuclear acid probe comprises the following steps: preparing numerous probes with different sequences and marking the probes with X; preparing numerous quantum-dots composite sphere with different emission spectrum characteristics and marking the sphere with Y; integrating probes marked with X and quantum-dots composite sphere marked with Y through specificity of X and Y and carrying out coupling on the probes and sphere; applied to multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, the probeof the invention can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, increase detection speed and prevent false positive result from occurring.

The invention relates to a dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis. The dedicated dispersion liquid for preparing high-resolution chromosome for karyotyping analysis is characterized by comprising the following components: 0.05-1mmol / L of Tris-HCL in pH of 7.5 to 10.0 and 2-8g / L of KCl, wherein the solvent is tri-distilled water. The dedicateddispersion liquid for preparing high-resolution chromosome for karyotyping analysis disclosed by the invention has good spread dispersion effect on the lymphocyte chromosome, the intersection and overlapping number between the chromosomes are reduced for the high-resolution and ultra-high-resolution karyotype analysis, and the quality for analyzing the karyotype is greatly improved.

The invention discloses an analysis method of sharp leaf CinnamomumKanehirae Hay karyotype based on root tips. The method comprises the following steps: collecting a robust, white and tender root tipof a tissue culture seedling of CinnamomumKanehirae Hay in a sunny morning to serve as a test material, pretreating, fixing and storing the test material, performing fixation, anterior hyposmosis, enzymolysis, post hyposmosis and smearing by using an enzymolysis wall removal hyposmosis method to obtain a better chromosome specimen, wherein the enzymolysis is performed by using a mixture of 4% cellulase and 5% pectinase, and performing Giemsa staining and microscopic examination. The method is simple and easy to operate, is suitable for the analysis method of sharp leaf CinnamomumKanehirae Haykaryotype with in-situ PCR, FISH operations, and provides a technical basis for the cytological study of the CinnamomumKanehirae Hay. The karyotype analysis method and the karyotype of the sharp leafCinnamomumKanehirae Hay are reported in the invention for the first time, which indicates that the CinnamomumKanehirae Hay has 24 chromosomes, the karyotype formula is 2n=2x=24=22m+2sm, the asymmetrycoefficient of the karyotype is 55.81%, and the karyotype belongs to a type 2B and belongs to relatively primitive species. A certain theoretical basis is provided for the origin, evolution and genetic breeding of the sharp leaf CinnamomumKanehirae Hay.